Loss of Gene Information: Discrepancies between RNA Sequencing, cDNA Microarray, and qRT-PCR

Loss of Gene Information: Discrepancies between RNA Sequencing, cDNA Microarray, and qRT-PCR

Molecular analyses of normal and diseased cells give insight into changes in gene expression and help in understanding the background of pathophysiological processes. Years after cDNA microarrays were established in research, RNA sequencing (RNA-seq) became a key method of quantitatively measuring t...

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Bibliographic Details
Main Authors: Nicole Rachinger, Stefan Fischer, Ines Böhme, Lisa Linck-Paulus, Silke Kuphal, Melanie Kappelmann-Fenzl, Anja K. Bosserhoff
Format: Article
Language:English
Published: MDPI AG 2021-08-01
Series:International Journal of Molecular Sciences
Subjects:
transcriptome analysis
RNA sequencing
cDNA microarray
qRT-PCR
secondary structure
free energy of the RNA secondary structure
Online Access:https://www.mdpi.com/1422-0067/22/17/9349

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