The detection of mixed tuberculosis infections using culture filtrate and resuscitation promoting factor deficient filtrate
Tuberculosis (TB) infected individuals harbor a heterogenous population of differentially culturable tubercle bacilli (DCTB). Herein, we describe how DCTB assays using culture filtrate either containing or deficient in resuscitation promoting factors can uncover mixed infections. We demonstrate that...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1072073/full |
| _version_ | 1828184604557705216 |
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| author | Melissa D. Chengalroyen Germar M. Beukes Kennedy Otwombe Bhavna G. Gordhan Neil Martinson Neil Martinson Neil Martinson Bavesh Kana |
| author_facet | Melissa D. Chengalroyen Germar M. Beukes Kennedy Otwombe Bhavna G. Gordhan Neil Martinson Neil Martinson Neil Martinson Bavesh Kana |
| author_sort | Melissa D. Chengalroyen |
| collection | DOAJ |
| description | Tuberculosis (TB) infected individuals harbor a heterogenous population of differentially culturable tubercle bacilli (DCTB). Herein, we describe how DCTB assays using culture filtrate either containing or deficient in resuscitation promoting factors can uncover mixed infections. We demonstrate that Mycobacterium tuberculosis (Mtb) strain genotypes can be separated in DCTB assays based on their selective requirement for growth stimulatory factors. Beijing mixed infections appear to be associated with a higher bacterial load and reduced reliance on growth stimulatory factors. These data have important implications for identifying mixed infections and hetero-resistance, which in turn can affect selection of treatment regimen and establishment of transmission links. |
| first_indexed | 2024-04-12T06:52:42Z |
| format | Article |
| id | doaj.art-5f74d09d5ad24715a72df06cb508fc34 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-12T06:52:42Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-5f74d09d5ad24715a72df06cb508fc342022-12-22T03:43:17ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-11-011210.3389/fcimb.2022.10720731072073The detection of mixed tuberculosis infections using culture filtrate and resuscitation promoting factor deficient filtrateMelissa D. Chengalroyen0Germar M. Beukes1Kennedy Otwombe2Bhavna G. Gordhan3Neil Martinson4Neil Martinson5Neil Martinson6Bavesh Kana7National Health Laboratory Service, DST/NRF Centre of Excellence for Biomedical TB Research, University of the Witwatersrand, Johannesburg, South AfricaNational Health Laboratory Service, DST/NRF Centre of Excellence for Biomedical TB Research, University of the Witwatersrand, Johannesburg, South AfricaPerinatal HIV Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South AfricaNational Health Laboratory Service, DST/NRF Centre of Excellence for Biomedical TB Research, University of the Witwatersrand, Johannesburg, South AfricaNational Health Laboratory Service, DST/NRF Centre of Excellence for Biomedical TB Research, University of the Witwatersrand, Johannesburg, South AfricaPerinatal HIV Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South AfricaCenter for TB Research, Johns Hopkins University, Baltimore, MD, United StatesNational Health Laboratory Service, DST/NRF Centre of Excellence for Biomedical TB Research, University of the Witwatersrand, Johannesburg, South AfricaTuberculosis (TB) infected individuals harbor a heterogenous population of differentially culturable tubercle bacilli (DCTB). Herein, we describe how DCTB assays using culture filtrate either containing or deficient in resuscitation promoting factors can uncover mixed infections. We demonstrate that Mycobacterium tuberculosis (Mtb) strain genotypes can be separated in DCTB assays based on their selective requirement for growth stimulatory factors. Beijing mixed infections appear to be associated with a higher bacterial load and reduced reliance on growth stimulatory factors. These data have important implications for identifying mixed infections and hetero-resistance, which in turn can affect selection of treatment regimen and establishment of transmission links.https://www.frontiersin.org/articles/10.3389/fcimb.2022.1072073/fullresuscitation promoting factorMycobacterium tuberculosismixed infectiondifferentially culturable tubercle bacteriamost probable number assay |
| spellingShingle | Melissa D. Chengalroyen Germar M. Beukes Kennedy Otwombe Bhavna G. Gordhan Neil Martinson Neil Martinson Neil Martinson Bavesh Kana The detection of mixed tuberculosis infections using culture filtrate and resuscitation promoting factor deficient filtrate Frontiers in Cellular and Infection Microbiology resuscitation promoting factor Mycobacterium tuberculosis mixed infection differentially culturable tubercle bacteria most probable number assay |
| title | The detection of mixed tuberculosis infections using culture filtrate and resuscitation promoting factor deficient filtrate |
| title_full | The detection of mixed tuberculosis infections using culture filtrate and resuscitation promoting factor deficient filtrate |
| title_fullStr | The detection of mixed tuberculosis infections using culture filtrate and resuscitation promoting factor deficient filtrate |
| title_full_unstemmed | The detection of mixed tuberculosis infections using culture filtrate and resuscitation promoting factor deficient filtrate |
| title_short | The detection of mixed tuberculosis infections using culture filtrate and resuscitation promoting factor deficient filtrate |
| title_sort | detection of mixed tuberculosis infections using culture filtrate and resuscitation promoting factor deficient filtrate |
| topic | resuscitation promoting factor Mycobacterium tuberculosis mixed infection differentially culturable tubercle bacteria most probable number assay |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1072073/full |
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