Investigation of Volatile Compounds in Combination with Multivariate Analysis for the Characterization of Monofloral Honeys

Lately there has been a growing demand for monofloral honeys with distinctive properties. Considering the limitations of pollen analysis, the volatile profile of honey has been proposed as a helpful supplementary tool for the confirmation of monoflorality; however, research remains regarding the vol...

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Main Authors: Chrysoula Tananaki, Vasilios Liolios, Dimitrios Kanelis, Maria Anna Rodopoulou
Format: Article
Language:English
Published: MDPI AG 2021-12-01
Series:Applied Sciences
Subjects:
Online Access:https://www.mdpi.com/2076-3417/12/1/264
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author Chrysoula Tananaki
Vasilios Liolios
Dimitrios Kanelis
Maria Anna Rodopoulou
author_facet Chrysoula Tananaki
Vasilios Liolios
Dimitrios Kanelis
Maria Anna Rodopoulou
author_sort Chrysoula Tananaki
collection DOAJ
description Lately there has been a growing demand for monofloral honeys with distinctive properties. Considering the limitations of pollen analysis, the volatile profile of honey has been proposed as a helpful supplementary tool for the confirmation of monoflorality; however, research remains regarding the volatile markers that may characterize the monofloral honey types. Therefore, in this study, we tried to expand the research by investigating the aroma profiles of five monofloral honey types (fir, pine, erica, thyme, cotton) and discriminate them through chemometric approach. A purge and trap–gas chromatograph–mass spectrometer system was used for the extraction, separation, and identification of volatile and semi-volatile compounds. Thyme honey had the richest quantitatively aroma profile, with 97 volatile compounds, whereas fir and cotton honeys had 65 and 60 volatile compounds, respectively. From a total of 124 compounds, the 38 were detected in all the studied honey types. Thyme honey was distinguished by the presence (or percentage participation) of benzeneacetaldehyde, benzealdehyde, and benzyl nitrile; erica honey of isophorone and furfural; cotton honey of 1-butanol, 2-methyl, 1-pentanol, and 4-methyl-; and honeydew honeys of α-pinene, octane, and nonanal. The discriminant analysis confirmed that the percentage participation of volatile compounds may lead to the discrimination of the studied monofloral honey types.
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spelling doaj.art-5f756890169646a1ad3bc45f98b750132023-11-23T11:10:17ZengMDPI AGApplied Sciences2076-34172021-12-0112126410.3390/app12010264Investigation of Volatile Compounds in Combination with Multivariate Analysis for the Characterization of Monofloral HoneysChrysoula Tananaki0Vasilios Liolios1Dimitrios Kanelis2Maria Anna Rodopoulou3Laboratory of Apiculture-Sericulture, Aristotle University of Thessaloniki, 57001 Thessalonik, GreeceLaboratory of Apiculture-Sericulture, Aristotle University of Thessaloniki, 57001 Thessalonik, GreeceLaboratory of Apiculture-Sericulture, Aristotle University of Thessaloniki, 57001 Thessalonik, GreeceLaboratory of Apiculture-Sericulture, Aristotle University of Thessaloniki, 57001 Thessalonik, GreeceLately there has been a growing demand for monofloral honeys with distinctive properties. Considering the limitations of pollen analysis, the volatile profile of honey has been proposed as a helpful supplementary tool for the confirmation of monoflorality; however, research remains regarding the volatile markers that may characterize the monofloral honey types. Therefore, in this study, we tried to expand the research by investigating the aroma profiles of five monofloral honey types (fir, pine, erica, thyme, cotton) and discriminate them through chemometric approach. A purge and trap–gas chromatograph–mass spectrometer system was used for the extraction, separation, and identification of volatile and semi-volatile compounds. Thyme honey had the richest quantitatively aroma profile, with 97 volatile compounds, whereas fir and cotton honeys had 65 and 60 volatile compounds, respectively. From a total of 124 compounds, the 38 were detected in all the studied honey types. Thyme honey was distinguished by the presence (or percentage participation) of benzeneacetaldehyde, benzealdehyde, and benzyl nitrile; erica honey of isophorone and furfural; cotton honey of 1-butanol, 2-methyl, 1-pentanol, and 4-methyl-; and honeydew honeys of α-pinene, octane, and nonanal. The discriminant analysis confirmed that the percentage participation of volatile compounds may lead to the discrimination of the studied monofloral honey types.https://www.mdpi.com/2076-3417/12/1/264volatile compoundsvolatile profilemonofloral honeysdiscriminant analysis
spellingShingle Chrysoula Tananaki
Vasilios Liolios
Dimitrios Kanelis
Maria Anna Rodopoulou
Investigation of Volatile Compounds in Combination with Multivariate Analysis for the Characterization of Monofloral Honeys
Applied Sciences
volatile compounds
volatile profile
monofloral honeys
discriminant analysis
title Investigation of Volatile Compounds in Combination with Multivariate Analysis for the Characterization of Monofloral Honeys
title_full Investigation of Volatile Compounds in Combination with Multivariate Analysis for the Characterization of Monofloral Honeys
title_fullStr Investigation of Volatile Compounds in Combination with Multivariate Analysis for the Characterization of Monofloral Honeys
title_full_unstemmed Investigation of Volatile Compounds in Combination with Multivariate Analysis for the Characterization of Monofloral Honeys
title_short Investigation of Volatile Compounds in Combination with Multivariate Analysis for the Characterization of Monofloral Honeys
title_sort investigation of volatile compounds in combination with multivariate analysis for the characterization of monofloral honeys
topic volatile compounds
volatile profile
monofloral honeys
discriminant analysis
url https://www.mdpi.com/2076-3417/12/1/264
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AT dimitrioskanelis investigationofvolatilecompoundsincombinationwithmultivariateanalysisforthecharacterizationofmonofloralhoneys
AT mariaannarodopoulou investigationofvolatilecompoundsincombinationwithmultivariateanalysisforthecharacterizationofmonofloralhoneys