Effect of Culture Medium Incorporated with Ocimum sanctum Extract in Enhancing Anti-MRSA activity of Endophytic Fungus, Lasiodiplodia pseudotheobromae
The effect of incorporating plant extract in the culture medium on anti-MRSA activity of Lasiodiplodia pseudotheobromae IBRL OS-64, was investigated in the present study. On disk diffusion assay, the ethyl acetate fungal extract from culture medium supplemented with host plant extract (HPE) of Ocimu...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2021-09-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/effect-of-culture-medium-incorporated-with-ocimum-sanctum-extract-in-enhancing-anti-mrsa-activity-of-endophytic-fungus-lasiodiplodia-pseudotheobromae/ |
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| author | Mohd Taufiq Mat Jalil Nurul Aili Zakaria Darah Ibrahim |
| author_facet | Mohd Taufiq Mat Jalil Nurul Aili Zakaria Darah Ibrahim |
| author_sort | Mohd Taufiq Mat Jalil |
| collection | DOAJ |
| description | The effect of incorporating plant extract in the culture medium on anti-MRSA activity of Lasiodiplodia pseudotheobromae IBRL OS-64, was investigated in the present study. On disk diffusion assay, the ethyl acetate fungal extract from culture medium supplemented with host plant extract (HPE) of Ocimum sanctum leaves demonstrated good anti-MRSA activity with a diameter inhibition zone of 22.6±0.6 mm. Meanwhile, the minimal inhibition concentration (MIC) values of the extract from YES broth and YES broth incorporated with HPE were 1000 µg/mL and 250 µg/mL, respectively. The MBC values were 8000 µg/mL and 500 µg/mL, respectively. The YES + HPE extracts exerted bactericidal effect against the test bacteria since the MBC/MIC ratio was less than or equal to 4. The time-kill study revealed a 90% of growth reduction of MRSA ATCC 33591 after 16 h exposure to the fungal extract cultured in YES + HPE. Ironically, for fungal extract grown in YES broth, time-kill curve showed a regrowth pattern of bacterial cells after 24 h exposed to the extract. Therefore, the present study suggested that the addition of HPE in the culture medium could enhance the anti-MRSA activity of endophytic fungus, L. pseudotheobromae IBRL OS-64 against MRSA ATCC 33591. |
| first_indexed | 2024-12-17T22:40:37Z |
| format | Article |
| id | doaj.art-5f75bdd594dd4889ac9bf6955a9b946f |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-17T22:40:37Z |
| publishDate | 2021-09-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-5f75bdd594dd4889ac9bf6955a9b946f2022-12-21T21:29:56ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2021-09-011531398140810.22207/JPAM.15.3.32Effect of Culture Medium Incorporated with Ocimum sanctum Extract in Enhancing Anti-MRSA activity of Endophytic Fungus, Lasiodiplodia pseudotheobromaeMohd Taufiq Mat Jalilhttps://orcid.org/0000-0002-3651-9424Nurul Aili Zakariahttps://orcid.org/0000-0002-0364-3891Darah Ibrahimhttps://orcid.org/0000-0003-3855-3666The effect of incorporating plant extract in the culture medium on anti-MRSA activity of Lasiodiplodia pseudotheobromae IBRL OS-64, was investigated in the present study. On disk diffusion assay, the ethyl acetate fungal extract from culture medium supplemented with host plant extract (HPE) of Ocimum sanctum leaves demonstrated good anti-MRSA activity with a diameter inhibition zone of 22.6±0.6 mm. Meanwhile, the minimal inhibition concentration (MIC) values of the extract from YES broth and YES broth incorporated with HPE were 1000 µg/mL and 250 µg/mL, respectively. The MBC values were 8000 µg/mL and 500 µg/mL, respectively. The YES + HPE extracts exerted bactericidal effect against the test bacteria since the MBC/MIC ratio was less than or equal to 4. The time-kill study revealed a 90% of growth reduction of MRSA ATCC 33591 after 16 h exposure to the fungal extract cultured in YES + HPE. Ironically, for fungal extract grown in YES broth, time-kill curve showed a regrowth pattern of bacterial cells after 24 h exposed to the extract. Therefore, the present study suggested that the addition of HPE in the culture medium could enhance the anti-MRSA activity of endophytic fungus, L. pseudotheobromae IBRL OS-64 against MRSA ATCC 33591.https://microbiologyjournal.org/effect-of-culture-medium-incorporated-with-ocimum-sanctum-extract-in-enhancing-anti-mrsa-activity-of-endophytic-fungus-lasiodiplodia-pseudotheobromae/anti-mrsa activitylasiodiplodia pseudotheobromaeocimum sanctumminimal inhibition concentration (mic)minimal bactericidal concentration (mbc)time-kill study |
| spellingShingle | Mohd Taufiq Mat Jalil Nurul Aili Zakaria Darah Ibrahim Effect of Culture Medium Incorporated with Ocimum sanctum Extract in Enhancing Anti-MRSA activity of Endophytic Fungus, Lasiodiplodia pseudotheobromae Journal of Pure and Applied Microbiology anti-mrsa activity lasiodiplodia pseudotheobromae ocimum sanctum minimal inhibition concentration (mic) minimal bactericidal concentration (mbc) time-kill study |
| title | Effect of Culture Medium Incorporated with Ocimum sanctum Extract in Enhancing Anti-MRSA activity of Endophytic Fungus, Lasiodiplodia pseudotheobromae |
| title_full | Effect of Culture Medium Incorporated with Ocimum sanctum Extract in Enhancing Anti-MRSA activity of Endophytic Fungus, Lasiodiplodia pseudotheobromae |
| title_fullStr | Effect of Culture Medium Incorporated with Ocimum sanctum Extract in Enhancing Anti-MRSA activity of Endophytic Fungus, Lasiodiplodia pseudotheobromae |
| title_full_unstemmed | Effect of Culture Medium Incorporated with Ocimum sanctum Extract in Enhancing Anti-MRSA activity of Endophytic Fungus, Lasiodiplodia pseudotheobromae |
| title_short | Effect of Culture Medium Incorporated with Ocimum sanctum Extract in Enhancing Anti-MRSA activity of Endophytic Fungus, Lasiodiplodia pseudotheobromae |
| title_sort | effect of culture medium incorporated with ocimum sanctum extract in enhancing anti mrsa activity of endophytic fungus lasiodiplodia pseudotheobromae |
| topic | anti-mrsa activity lasiodiplodia pseudotheobromae ocimum sanctum minimal inhibition concentration (mic) minimal bactericidal concentration (mbc) time-kill study |
| url | https://microbiologyjournal.org/effect-of-culture-medium-incorporated-with-ocimum-sanctum-extract-in-enhancing-anti-mrsa-activity-of-endophytic-fungus-lasiodiplodia-pseudotheobromae/ |
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