Vancomycin resistant Streptococcus equi subsp. equi isolated from equines suffering from respiratory manifestation in Egypt
Background and Aim: Upper respiratory tract infections are common in horses and can be caused by a variety of pathogens, mainly Streptococcus equi subsp. equi, which are a significant equine pathogen causing major health issues as well as financial losses to the equine industry. This study aimed to...
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Veterinary World
2021-07-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.14/July-2021/12.pdf |
| _version_ | 1818648553546842112 |
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| author | Amany A. Arafa Riham H. Hedia Nagwa S. Ata Eman S. Ibrahim |
| author_facet | Amany A. Arafa Riham H. Hedia Nagwa S. Ata Eman S. Ibrahim |
| author_sort | Amany A. Arafa |
| collection | DOAJ |
| description | Background and Aim: Upper respiratory tract infections are common in horses and can be caused by a variety of pathogens, mainly Streptococcus equi subsp. equi, which are a significant equine pathogen causing major health issues as well as financial losses to the equine industry. This study aimed to determine the prevalence of Streptococcal bacteria in equines in Egypt, and characterize vancomycin-resistant S. equi subsp. equi phenotypically and genotypically.
Materials and Methods: S. equi subsp. equi was isolated from internal nares of horses. All strains were confirmed by polymerase chain reaction-based detection of Streptococcus genus-specific 16S rRNA, sodA and seeI genes. Antibiotic susceptibility was determined phenotypically using the disk diffusion method. Genotypic detection of antibiotic resistance genes was performed by analyzing as β-lactamase resistance (blaZ), tetracycline resistance (tetK), vancomycin resistance (vanA), and chloramphenicol resistance (fexA).
Results: Eight streptococcal isolates were confirmed as S. equi subsp. equi. The genotypic characterization of antibiotic resistance showed resistance to vanA and tetK, with a frequency of 87.5% and 12.5%, respectively, while the frequency of sensitivity was 100% for blaz gene and fexA gene.
Conclusion: In this study, we assessed vancomycin-resistant S. equi subsp. equi from equines suffering from respiratory manifestation in Egypt. |
| first_indexed | 2024-12-17T01:20:16Z |
| format | Article |
| id | doaj.art-5f76108e28af40bc80ca32deca145a6f |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-17T01:20:16Z |
| publishDate | 2021-07-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-5f76108e28af40bc80ca32deca145a6f2022-12-21T22:08:51ZengVeterinary WorldVeterinary World0972-89882231-09162021-07-011471808181410.14202/vetworld.2021.1808-1814Vancomycin resistant Streptococcus equi subsp. equi isolated from equines suffering from respiratory manifestation in EgyptAmany A. Arafa0https://orcid.org/0000-0003-4156-8068Riham H. Hedia1https://orcid.org/0000-0001-5868-0473Nagwa S. Ata2https://orcid.org/0000-0002-3477-5002Eman S. Ibrahim3https://orcid.org/0000-0002-6315-4512Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.Background and Aim: Upper respiratory tract infections are common in horses and can be caused by a variety of pathogens, mainly Streptococcus equi subsp. equi, which are a significant equine pathogen causing major health issues as well as financial losses to the equine industry. This study aimed to determine the prevalence of Streptococcal bacteria in equines in Egypt, and characterize vancomycin-resistant S. equi subsp. equi phenotypically and genotypically. Materials and Methods: S. equi subsp. equi was isolated from internal nares of horses. All strains were confirmed by polymerase chain reaction-based detection of Streptococcus genus-specific 16S rRNA, sodA and seeI genes. Antibiotic susceptibility was determined phenotypically using the disk diffusion method. Genotypic detection of antibiotic resistance genes was performed by analyzing as β-lactamase resistance (blaZ), tetracycline resistance (tetK), vancomycin resistance (vanA), and chloramphenicol resistance (fexA). Results: Eight streptococcal isolates were confirmed as S. equi subsp. equi. The genotypic characterization of antibiotic resistance showed resistance to vanA and tetK, with a frequency of 87.5% and 12.5%, respectively, while the frequency of sensitivity was 100% for blaz gene and fexA gene. Conclusion: In this study, we assessed vancomycin-resistant S. equi subsp. equi from equines suffering from respiratory manifestation in Egypt.http://www.veterinaryworld.org/Vol.14/July-2021/12.pdfantibiotic resistanceequinespolymerase chain reactionstreptococcus equi subsp. equivancomycin |
| spellingShingle | Amany A. Arafa Riham H. Hedia Nagwa S. Ata Eman S. Ibrahim Vancomycin resistant Streptococcus equi subsp. equi isolated from equines suffering from respiratory manifestation in Egypt Veterinary World antibiotic resistance equines polymerase chain reaction streptococcus equi subsp. equi vancomycin |
| title | Vancomycin resistant Streptococcus equi subsp. equi isolated from equines suffering from respiratory manifestation in Egypt |
| title_full | Vancomycin resistant Streptococcus equi subsp. equi isolated from equines suffering from respiratory manifestation in Egypt |
| title_fullStr | Vancomycin resistant Streptococcus equi subsp. equi isolated from equines suffering from respiratory manifestation in Egypt |
| title_full_unstemmed | Vancomycin resistant Streptococcus equi subsp. equi isolated from equines suffering from respiratory manifestation in Egypt |
| title_short | Vancomycin resistant Streptococcus equi subsp. equi isolated from equines suffering from respiratory manifestation in Egypt |
| title_sort | vancomycin resistant streptococcus equi subsp equi isolated from equines suffering from respiratory manifestation in egypt |
| topic | antibiotic resistance equines polymerase chain reaction streptococcus equi subsp. equi vancomycin |
| url | http://www.veterinaryworld.org/Vol.14/July-2021/12.pdf |
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