The discovery of Candidatus Nanopusillus phoceensis sheds light on the diversity of the microbiota nanoarchaea
Summary: To further assess the spectrum of nanoarchaea in human microbiota, we prospectively searched for nanoarchaea in 110 leftover stool specimens, using the complementary approaches of PCR-sequencing screening, fluorescent in situ hybridization, scanning electron microscopy and metagenomics. The...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224007090 |
| _version_ | 1827292116214087680 |
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| author | Yasmine Hassani Gerard Aboudharam Michel Drancourt Ghiles Grine |
| author_facet | Yasmine Hassani Gerard Aboudharam Michel Drancourt Ghiles Grine |
| author_sort | Yasmine Hassani |
| collection | DOAJ |
| description | Summary: To further assess the spectrum of nanoarchaea in human microbiota, we prospectively searched for nanoarchaea in 110 leftover stool specimens, using the complementary approaches of PCR-sequencing screening, fluorescent in situ hybridization, scanning electron microscopy and metagenomics. These investigations yielded a nanoarchaea, Candidatus Nanopusillus phoceensis sp. nov., detected in stool samples by specific PCR-based assays. Microscopic observations indicated its close contact with the archaea Methanobrevibacter smithii. Genomic sequencing revealed 607,775-bp contig with 24.5% G + C content encoding 30 tRNAs, 3 rRNA genes, and 1,403 coding DNA sequences, of which 719 were assigned to clusters of orthologous groups.Ca. Nanopusillus phoceensis is only the second nanoarchaea to be detected in humans, expanding our knowledge of the repertoire of nanoarchaea associated with the human microbiota and encouraging further research to explore the repertoire of this emerging group of nanomicrobes in clinical samples. |
| first_indexed | 2024-04-24T12:51:04Z |
| format | Article |
| id | doaj.art-5f76aa6b9201424789e06594c7b63500 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-24T12:51:04Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-5f76aa6b9201424789e06594c7b635002024-04-06T04:40:18ZengElsevieriScience2589-00422024-04-01274109488The discovery of Candidatus Nanopusillus phoceensis sheds light on the diversity of the microbiota nanoarchaeaYasmine Hassani0Gerard Aboudharam1Michel Drancourt2Ghiles Grine3Aix-Marseille Université, IRD, MEPHI, IHU Méditerranée Infection, 13005 Marseille, France; IHU Méditerranée Infection, 13005 Marseille, FranceAix-Marseille Université, IRD, MEPHI, IHU Méditerranée Infection, 13005 Marseille, France; Ecole de Médecine Dentaire, Aix-Marseille Université, 13005 Marseille, FranceAix-Marseille Université, IRD, MEPHI, IHU Méditerranée Infection, 13005 Marseille, France; IHU Méditerranée Infection, 13005 Marseille, FranceAix-Marseille Université, IRD, MEPHI, IHU Méditerranée Infection, 13005 Marseille, France; Ecole de Médecine Dentaire, Aix-Marseille Université, 13005 Marseille, France; Corresponding authorSummary: To further assess the spectrum of nanoarchaea in human microbiota, we prospectively searched for nanoarchaea in 110 leftover stool specimens, using the complementary approaches of PCR-sequencing screening, fluorescent in situ hybridization, scanning electron microscopy and metagenomics. These investigations yielded a nanoarchaea, Candidatus Nanopusillus phoceensis sp. nov., detected in stool samples by specific PCR-based assays. Microscopic observations indicated its close contact with the archaea Methanobrevibacter smithii. Genomic sequencing revealed 607,775-bp contig with 24.5% G + C content encoding 30 tRNAs, 3 rRNA genes, and 1,403 coding DNA sequences, of which 719 were assigned to clusters of orthologous groups.Ca. Nanopusillus phoceensis is only the second nanoarchaea to be detected in humans, expanding our knowledge of the repertoire of nanoarchaea associated with the human microbiota and encouraging further research to explore the repertoire of this emerging group of nanomicrobes in clinical samples.http://www.sciencedirect.com/science/article/pii/S2589004224007090Biological sciencesMicrobiologyBacteriologyMicrobiome |
| spellingShingle | Yasmine Hassani Gerard Aboudharam Michel Drancourt Ghiles Grine The discovery of Candidatus Nanopusillus phoceensis sheds light on the diversity of the microbiota nanoarchaea iScience Biological sciences Microbiology Bacteriology Microbiome |
| title | The discovery of Candidatus Nanopusillus phoceensis sheds light on the diversity of the microbiota nanoarchaea |
| title_full | The discovery of Candidatus Nanopusillus phoceensis sheds light on the diversity of the microbiota nanoarchaea |
| title_fullStr | The discovery of Candidatus Nanopusillus phoceensis sheds light on the diversity of the microbiota nanoarchaea |
| title_full_unstemmed | The discovery of Candidatus Nanopusillus phoceensis sheds light on the diversity of the microbiota nanoarchaea |
| title_short | The discovery of Candidatus Nanopusillus phoceensis sheds light on the diversity of the microbiota nanoarchaea |
| title_sort | discovery of candidatus nanopusillus phoceensis sheds light on the diversity of the microbiota nanoarchaea |
| topic | Biological sciences Microbiology Bacteriology Microbiome |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224007090 |
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