Isolation and Cultivation of Carotenoid-Producing Strains from Tidal Flat Sediment and Proposal of <i>Croceibacterium aestuarii</i> sp. nov., a Novel Carotenoid-Producing Species in the Family <i>Erythrobacteraceae</i>
Carotenoids are extensively used in drugs, cosmetics, nutrients, and foods, owing to their antioxidant and anti-inflammatory characteristics. Diverse marine heterotrophic prokaryotes can accumulate carotenoids and become promising alternatives for the advancement of carotenoids production. In this r...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Journal of Marine Science and Engineering |
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| Online Access: | https://www.mdpi.com/2077-1312/12/1/99 |
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| author | Xiao-Yan Sun Han Dong Yu Zhang Jia-Wei Gao Peng Zhou Cong Sun Lin Xu |
| author_facet | Xiao-Yan Sun Han Dong Yu Zhang Jia-Wei Gao Peng Zhou Cong Sun Lin Xu |
| author_sort | Xiao-Yan Sun |
| collection | DOAJ |
| description | Carotenoids are extensively used in drugs, cosmetics, nutrients, and foods, owing to their antioxidant and anti-inflammatory characteristics. Diverse marine heterotrophic prokaryotes can accumulate carotenoids and become promising alternatives for the advancement of carotenoids production. In this research, 55 strains were isolated and cultivated from tidal flat sediment in Zhoushan and classified into the phyla <i>Pseudomonadota</i> (<i>n</i> = 24), <i>Bacillota</i> (<i>n</i> = 18), <i>Bacteroidota</i> (<i>n</i> = 9), and <i>Actinomycetota</i> (<i>n</i> = 4). Nine of them accumulated carotenoids, and most of them belonged to the families <i>Flavobacteriaceae</i> (<i>n</i> = 4) and <i>Erythrobacteraceae</i> (<i>n</i> = 4). Among those carotenoid-producing strains, one strain, designated as D39<sup>T</sup>, was proposed as one novel species belonging to the genus <i>Croceibacterium</i> through polyphasic taxonomy approaches. Genomic annotations and carotenoid compound determinations revealed that strain D39<sup>T</sup> encoded <i>crtEBIYZG</i> genes and mainly accumulated zeaxanthin as major carotenoids. Furthermore, carotenoid biosynthesis pathway in the majority of <i>Croceibacterium</i> strains were identical with that in the strain D39<sup>T</sup>, implying that <i>Croceibacterium</i> members can be sources of producing zeaxanthin. This study enhances knowledge of microbial biodiversity in tidal flats, proposes a novel carotenoid-producing <i>Croceibacterium</i> species, and elucidates carotenoid biosynthesis pathway in the genus <i>Croceibacterium</i>, which contribute to enriching marine carotenoid-producing strains and promoting a comprehensive insight into genomic contents of them. |
| first_indexed | 2024-03-08T10:46:13Z |
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| institution | Directory Open Access Journal |
| issn | 2077-1312 |
| language | English |
| last_indexed | 2024-03-08T10:46:13Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
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| series | Journal of Marine Science and Engineering |
| spelling | doaj.art-5f77d4e49c0a48c0a734f1228bb8f92f2024-01-26T17:15:50ZengMDPI AGJournal of Marine Science and Engineering2077-13122024-01-011219910.3390/jmse12010099Isolation and Cultivation of Carotenoid-Producing Strains from Tidal Flat Sediment and Proposal of <i>Croceibacterium aestuarii</i> sp. nov., a Novel Carotenoid-Producing Species in the Family <i>Erythrobacteraceae</i>Xiao-Yan Sun0Han Dong1Yu Zhang2Jia-Wei Gao3Peng Zhou4Cong Sun5Lin Xu6College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaKey Laboratory of Marine Ecosystem Dynamics, Ministry of Natural Resources & Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou 310012, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCarotenoids are extensively used in drugs, cosmetics, nutrients, and foods, owing to their antioxidant and anti-inflammatory characteristics. Diverse marine heterotrophic prokaryotes can accumulate carotenoids and become promising alternatives for the advancement of carotenoids production. In this research, 55 strains were isolated and cultivated from tidal flat sediment in Zhoushan and classified into the phyla <i>Pseudomonadota</i> (<i>n</i> = 24), <i>Bacillota</i> (<i>n</i> = 18), <i>Bacteroidota</i> (<i>n</i> = 9), and <i>Actinomycetota</i> (<i>n</i> = 4). Nine of them accumulated carotenoids, and most of them belonged to the families <i>Flavobacteriaceae</i> (<i>n</i> = 4) and <i>Erythrobacteraceae</i> (<i>n</i> = 4). Among those carotenoid-producing strains, one strain, designated as D39<sup>T</sup>, was proposed as one novel species belonging to the genus <i>Croceibacterium</i> through polyphasic taxonomy approaches. Genomic annotations and carotenoid compound determinations revealed that strain D39<sup>T</sup> encoded <i>crtEBIYZG</i> genes and mainly accumulated zeaxanthin as major carotenoids. Furthermore, carotenoid biosynthesis pathway in the majority of <i>Croceibacterium</i> strains were identical with that in the strain D39<sup>T</sup>, implying that <i>Croceibacterium</i> members can be sources of producing zeaxanthin. This study enhances knowledge of microbial biodiversity in tidal flats, proposes a novel carotenoid-producing <i>Croceibacterium</i> species, and elucidates carotenoid biosynthesis pathway in the genus <i>Croceibacterium</i>, which contribute to enriching marine carotenoid-producing strains and promoting a comprehensive insight into genomic contents of them.https://www.mdpi.com/2077-1312/12/1/99tidal flatmarine bacteria<i>Croceibacterium</i>polyphasic taxonomycarotenoidszeaxanthin |
| spellingShingle | Xiao-Yan Sun Han Dong Yu Zhang Jia-Wei Gao Peng Zhou Cong Sun Lin Xu Isolation and Cultivation of Carotenoid-Producing Strains from Tidal Flat Sediment and Proposal of <i>Croceibacterium aestuarii</i> sp. nov., a Novel Carotenoid-Producing Species in the Family <i>Erythrobacteraceae</i> Journal of Marine Science and Engineering tidal flat marine bacteria <i>Croceibacterium</i> polyphasic taxonomy carotenoids zeaxanthin |
| title | Isolation and Cultivation of Carotenoid-Producing Strains from Tidal Flat Sediment and Proposal of <i>Croceibacterium aestuarii</i> sp. nov., a Novel Carotenoid-Producing Species in the Family <i>Erythrobacteraceae</i> |
| title_full | Isolation and Cultivation of Carotenoid-Producing Strains from Tidal Flat Sediment and Proposal of <i>Croceibacterium aestuarii</i> sp. nov., a Novel Carotenoid-Producing Species in the Family <i>Erythrobacteraceae</i> |
| title_fullStr | Isolation and Cultivation of Carotenoid-Producing Strains from Tidal Flat Sediment and Proposal of <i>Croceibacterium aestuarii</i> sp. nov., a Novel Carotenoid-Producing Species in the Family <i>Erythrobacteraceae</i> |
| title_full_unstemmed | Isolation and Cultivation of Carotenoid-Producing Strains from Tidal Flat Sediment and Proposal of <i>Croceibacterium aestuarii</i> sp. nov., a Novel Carotenoid-Producing Species in the Family <i>Erythrobacteraceae</i> |
| title_short | Isolation and Cultivation of Carotenoid-Producing Strains from Tidal Flat Sediment and Proposal of <i>Croceibacterium aestuarii</i> sp. nov., a Novel Carotenoid-Producing Species in the Family <i>Erythrobacteraceae</i> |
| title_sort | isolation and cultivation of carotenoid producing strains from tidal flat sediment and proposal of i croceibacterium aestuarii i sp nov a novel carotenoid producing species in the family i erythrobacteraceae i |
| topic | tidal flat marine bacteria <i>Croceibacterium</i> polyphasic taxonomy carotenoids zeaxanthin |
| url | https://www.mdpi.com/2077-1312/12/1/99 |
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