Physico-Chemical Approaches to Investigate Surface Hydroxyls as Determinants of Molecular Initiating Events in Oxide Particle Toxicity
The study of molecular recognition patterns is crucial for understanding the interactions between inorganic (nano)particles and biomolecules. In this review we focus on hydroxyls (OH) exposed at the surface of oxide particles (OxPs) which can play a key role in molecular initiating events leading to...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/14/11482 |
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| author | Cristina Pavan Rosangela Santalucia Guillermo Escolano-Casado Piero Ugliengo Lorenzo Mino Francesco Turci |
| author_facet | Cristina Pavan Rosangela Santalucia Guillermo Escolano-Casado Piero Ugliengo Lorenzo Mino Francesco Turci |
| author_sort | Cristina Pavan |
| collection | DOAJ |
| description | The study of molecular recognition patterns is crucial for understanding the interactions between inorganic (nano)particles and biomolecules. In this review we focus on hydroxyls (OH) exposed at the surface of oxide particles (OxPs) which can play a key role in molecular initiating events leading to OxPs toxicity. We discuss here the main analytical methods available to characterize surface OH from a quantitative and qualitative point of view, covering thermogravimetry, titration, ζ potential measurements, and spectroscopic approaches (NMR, XPS). The importance of modelling techniques (MD, DFT) for an atomistic description of the interactions between membranes/proteins and OxPs surfaces is also discussed. From this background, we distilled a new approach methodology (NAM) based on the combination of IR spectroscopy and bioanalytical assays to investigate the molecular interactions of OxPs with biomolecules and membranes. This NAM has been already successfully applied to SiO<sub>2</sub> particles to identify the OH patterns responsible for the OxPs’ toxicity and can be conceivably extended to other surface-hydroxylated oxides. |
| first_indexed | 2024-03-11T01:00:37Z |
| format | Article |
| id | doaj.art-5f7cc7d8eb9c421bae294cd7b1a18223 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T01:00:37Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5f7cc7d8eb9c421bae294cd7b1a182232023-11-18T19:40:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124141148210.3390/ijms241411482Physico-Chemical Approaches to Investigate Surface Hydroxyls as Determinants of Molecular Initiating Events in Oxide Particle ToxicityCristina Pavan0Rosangela Santalucia1Guillermo Escolano-Casado2Piero Ugliengo3Lorenzo Mino4Francesco Turci5Department of Chemistry, University of Torino, Via Giuria 7, 10125 Torino, ItalyDepartment of Chemistry, University of Torino, Via Giuria 7, 10125 Torino, ItalyDepartment of Chemistry, University of Torino, Via Giuria 7, 10125 Torino, ItalyDepartment of Chemistry, University of Torino, Via Giuria 7, 10125 Torino, ItalyDepartment of Chemistry, University of Torino, Via Giuria 7, 10125 Torino, ItalyDepartment of Chemistry, University of Torino, Via Giuria 7, 10125 Torino, ItalyThe study of molecular recognition patterns is crucial for understanding the interactions between inorganic (nano)particles and biomolecules. In this review we focus on hydroxyls (OH) exposed at the surface of oxide particles (OxPs) which can play a key role in molecular initiating events leading to OxPs toxicity. We discuss here the main analytical methods available to characterize surface OH from a quantitative and qualitative point of view, covering thermogravimetry, titration, ζ potential measurements, and spectroscopic approaches (NMR, XPS). The importance of modelling techniques (MD, DFT) for an atomistic description of the interactions between membranes/proteins and OxPs surfaces is also discussed. From this background, we distilled a new approach methodology (NAM) based on the combination of IR spectroscopy and bioanalytical assays to investigate the molecular interactions of OxPs with biomolecules and membranes. This NAM has been already successfully applied to SiO<sub>2</sub> particles to identify the OH patterns responsible for the OxPs’ toxicity and can be conceivably extended to other surface-hydroxylated oxides.https://www.mdpi.com/1422-0067/24/14/11482silicametal oxide nanoparticlesmolecular interactionsurface chemistrymolecular recognition patternadsorption |
| spellingShingle | Cristina Pavan Rosangela Santalucia Guillermo Escolano-Casado Piero Ugliengo Lorenzo Mino Francesco Turci Physico-Chemical Approaches to Investigate Surface Hydroxyls as Determinants of Molecular Initiating Events in Oxide Particle Toxicity International Journal of Molecular Sciences silica metal oxide nanoparticles molecular interaction surface chemistry molecular recognition pattern adsorption |
| title | Physico-Chemical Approaches to Investigate Surface Hydroxyls as Determinants of Molecular Initiating Events in Oxide Particle Toxicity |
| title_full | Physico-Chemical Approaches to Investigate Surface Hydroxyls as Determinants of Molecular Initiating Events in Oxide Particle Toxicity |
| title_fullStr | Physico-Chemical Approaches to Investigate Surface Hydroxyls as Determinants of Molecular Initiating Events in Oxide Particle Toxicity |
| title_full_unstemmed | Physico-Chemical Approaches to Investigate Surface Hydroxyls as Determinants of Molecular Initiating Events in Oxide Particle Toxicity |
| title_short | Physico-Chemical Approaches to Investigate Surface Hydroxyls as Determinants of Molecular Initiating Events in Oxide Particle Toxicity |
| title_sort | physico chemical approaches to investigate surface hydroxyls as determinants of molecular initiating events in oxide particle toxicity |
| topic | silica metal oxide nanoparticles molecular interaction surface chemistry molecular recognition pattern adsorption |
| url | https://www.mdpi.com/1422-0067/24/14/11482 |
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