Optimization of Extraction Parameters of Phenolic Compounds from Sarcopoterium spinosum Leaves by Response Surface Methodology
The shrublands are very common in Urla-Çeşme-Karaburun peninsula located in the western point of Turkey. Prickly shrubby burnet (Sarcopoterium spinosum L.) is one of the common weed which has intensive thorns making its consumption for the local domestic animals. However, Sarcopoterium spinosum is a...
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| Format: | Article |
| Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2017-07-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/1195 |
| _version_ | 1797905604518346752 |
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| author | Ceren Sunguc Oguz Bayraktar İpek Erdogan Mehmet Emin Uslu |
| author_facet | Ceren Sunguc Oguz Bayraktar İpek Erdogan Mehmet Emin Uslu |
| author_sort | Ceren Sunguc |
| collection | DOAJ |
| description | The shrublands are very common in Urla-Çeşme-Karaburun peninsula located in the western point of Turkey. Prickly shrubby burnet (Sarcopoterium spinosum L.) is one of the common weed which has intensive thorns making its consumption for the local domestic animals. However, Sarcopoterium spinosum is a valuable and common medicinal plant in the Mediterranean region. Crude extract of S. spinosum leaves exhibited higher antioxidant activity, as 3143.5± 238.5 µM TEAC (Trolox Equivalent Antioxidant Capacity)/g dry weight (DW), when compared to other medicinal plants found in the literature. The aim of this study was to determine the effect of extraction parameters on the content and biological activity of the extract by response surface methodology (RSM) as well as to identify its major compounds. High Performance Liquid Chromatography (HPLC) was employed to investigate the phenolic content of S. spinosum extract. The composition of the phenolic contents including hyperoside and isoquercetin, the latter being the major component, in S. spinosum extract has been shown for the first time by HPLC. Antimicrobial activity of S. spinosum extract, identified by minimum inhibition concentration (MIC) assay, indicated that the crude extract had antifungal activity against Candida albicans. |
| first_indexed | 2024-04-10T10:07:54Z |
| format | Article |
| id | doaj.art-5f7d92d9cc844e3abdccff40407d2fe9 |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-04-10T10:07:54Z |
| publishDate | 2017-07-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-5f7d92d9cc844e3abdccff40407d2fe92023-02-15T16:22:23ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2017-07-015779279910.24925/turjaf.v5i7.792-799.1195569Optimization of Extraction Parameters of Phenolic Compounds from Sarcopoterium spinosum Leaves by Response Surface MethodologyCeren Sunguc0Oguz Bayraktar1İpek Erdogan2Mehmet Emin Uslu3Department of Biotechnology and Bioengineering, Faculty of Engineering, Izmir Institute of Technology, 35430 IzmirDepartment of Chemical Engineering, Faculty of Engineering, Ege University, 35040 IzmirDepartment of Biotechnology and Bioengineering, Faculty of Engineering, Izmir Institute of Technology, 35430 IzmirDepartment of Biomedical Equipment Technology, Soma Vocational School, Celal Bayar University, 45500 ManisaThe shrublands are very common in Urla-Çeşme-Karaburun peninsula located in the western point of Turkey. Prickly shrubby burnet (Sarcopoterium spinosum L.) is one of the common weed which has intensive thorns making its consumption for the local domestic animals. However, Sarcopoterium spinosum is a valuable and common medicinal plant in the Mediterranean region. Crude extract of S. spinosum leaves exhibited higher antioxidant activity, as 3143.5± 238.5 µM TEAC (Trolox Equivalent Antioxidant Capacity)/g dry weight (DW), when compared to other medicinal plants found in the literature. The aim of this study was to determine the effect of extraction parameters on the content and biological activity of the extract by response surface methodology (RSM) as well as to identify its major compounds. High Performance Liquid Chromatography (HPLC) was employed to investigate the phenolic content of S. spinosum extract. The composition of the phenolic contents including hyperoside and isoquercetin, the latter being the major component, in S. spinosum extract has been shown for the first time by HPLC. Antimicrobial activity of S. spinosum extract, identified by minimum inhibition concentration (MIC) assay, indicated that the crude extract had antifungal activity against Candida albicans.http://www.agrifoodscience.com/index.php/TURJAF/article/view/1195Extraction parametershyperosideisoquercetinphenolic contentprickly shrubby burnetSarcopoterium spinosum |
| spellingShingle | Ceren Sunguc Oguz Bayraktar İpek Erdogan Mehmet Emin Uslu Optimization of Extraction Parameters of Phenolic Compounds from Sarcopoterium spinosum Leaves by Response Surface Methodology Turkish Journal of Agriculture: Food Science and Technology Extraction parameters hyperoside isoquercetin phenolic content prickly shrubby burnet Sarcopoterium spinosum |
| title | Optimization of Extraction Parameters of Phenolic Compounds from Sarcopoterium spinosum Leaves by Response Surface Methodology |
| title_full | Optimization of Extraction Parameters of Phenolic Compounds from Sarcopoterium spinosum Leaves by Response Surface Methodology |
| title_fullStr | Optimization of Extraction Parameters of Phenolic Compounds from Sarcopoterium spinosum Leaves by Response Surface Methodology |
| title_full_unstemmed | Optimization of Extraction Parameters of Phenolic Compounds from Sarcopoterium spinosum Leaves by Response Surface Methodology |
| title_short | Optimization of Extraction Parameters of Phenolic Compounds from Sarcopoterium spinosum Leaves by Response Surface Methodology |
| title_sort | optimization of extraction parameters of phenolic compounds from sarcopoterium spinosum leaves by response surface methodology |
| topic | Extraction parameters hyperoside isoquercetin phenolic content prickly shrubby burnet Sarcopoterium spinosum |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/1195 |
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