Secretome-based identification of ULBP2 as a novel serum marker for pancreatic cancer detection.
<h4>Background</h4>To discover novel markers for improving the efficacy of pancreatic cancer (PC) diagnosis, the secretome of two PC cell lines (BxPC-3 and MIA PaCa-2) was profiled. UL16 binding protein 2 (ULBP2), one of the proteins identified in the PC cell secretome, was selected for...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625447/pdf/?tool=EBI |
| _version_ | 1818838013706240000 |
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| author | Ya-Ting Chang Chih-Ching Wu Yi-Ming Shyr Tse-Ching Chen Tsann-Long Hwang Ta-Sen Yeh Kai-Ping Chang Hao-Ping Liu Yu-Ling Liu Ming-Hung Tsai Yu-Sun Chang Jau-Song Yu |
| author_facet | Ya-Ting Chang Chih-Ching Wu Yi-Ming Shyr Tse-Ching Chen Tsann-Long Hwang Ta-Sen Yeh Kai-Ping Chang Hao-Ping Liu Yu-Ling Liu Ming-Hung Tsai Yu-Sun Chang Jau-Song Yu |
| author_sort | Ya-Ting Chang |
| collection | DOAJ |
| description | <h4>Background</h4>To discover novel markers for improving the efficacy of pancreatic cancer (PC) diagnosis, the secretome of two PC cell lines (BxPC-3 and MIA PaCa-2) was profiled. UL16 binding protein 2 (ULBP2), one of the proteins identified in the PC cell secretome, was selected for evaluation as a biomarker for PC detection because its mRNA level was also found to be significantly elevated in PC tissues.<h4>Methods</h4>ULBP2 expression in PC tissues from 67 patients was studied by immunohistochemistry. ULBP2 serum levels in 154 PC patients and 142 healthy controls were measured by bead-based immunoassay, and the efficacy of serum ULBP2 for PC detection was compared with the widely used serological PC marker carbohydrate antigen 19-9 (CA 19-9).<h4>Results</h4>Immunohistochemical analyses revealed an elevated expression of ULPB2 in PC tissues compared with adjacent non-cancerous tissues. Meanwhile, the serum levels of ULBP2 among all PC patients (n = 154) and in early-stage cancer patients were significantly higher than those in healthy controls (p<0.0001). The combination of ULBP2 and CA 19-9 outperformed each marker alone in distinguishing PC patients from healthy individuals. Importantly, an analysis of the area under receiver operating characteristic curves showed that ULBP2 was superior to CA 19-9 in discriminating patients with early-stage PC from healthy controls.<h4>Conclusions</h4>Collectively, our results indicate that ULBP2 may represent a novel and useful serum biomarker for pancreatic cancer primary screening. |
| first_indexed | 2024-12-19T03:31:39Z |
| format | Article |
| id | doaj.art-5f7f7fc9ee8142caaec05b9700b0dd8b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T03:31:39Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5f7f7fc9ee8142caaec05b9700b0dd8b2022-12-21T20:37:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0165e2002910.1371/journal.pone.0020029Secretome-based identification of ULBP2 as a novel serum marker for pancreatic cancer detection.Ya-Ting ChangChih-Ching WuYi-Ming ShyrTse-Ching ChenTsann-Long HwangTa-Sen YehKai-Ping ChangHao-Ping LiuYu-Ling LiuMing-Hung TsaiYu-Sun ChangJau-Song Yu<h4>Background</h4>To discover novel markers for improving the efficacy of pancreatic cancer (PC) diagnosis, the secretome of two PC cell lines (BxPC-3 and MIA PaCa-2) was profiled. UL16 binding protein 2 (ULBP2), one of the proteins identified in the PC cell secretome, was selected for evaluation as a biomarker for PC detection because its mRNA level was also found to be significantly elevated in PC tissues.<h4>Methods</h4>ULBP2 expression in PC tissues from 67 patients was studied by immunohistochemistry. ULBP2 serum levels in 154 PC patients and 142 healthy controls were measured by bead-based immunoassay, and the efficacy of serum ULBP2 for PC detection was compared with the widely used serological PC marker carbohydrate antigen 19-9 (CA 19-9).<h4>Results</h4>Immunohistochemical analyses revealed an elevated expression of ULPB2 in PC tissues compared with adjacent non-cancerous tissues. Meanwhile, the serum levels of ULBP2 among all PC patients (n = 154) and in early-stage cancer patients were significantly higher than those in healthy controls (p<0.0001). The combination of ULBP2 and CA 19-9 outperformed each marker alone in distinguishing PC patients from healthy individuals. Importantly, an analysis of the area under receiver operating characteristic curves showed that ULBP2 was superior to CA 19-9 in discriminating patients with early-stage PC from healthy controls.<h4>Conclusions</h4>Collectively, our results indicate that ULBP2 may represent a novel and useful serum biomarker for pancreatic cancer primary screening.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625447/pdf/?tool=EBI |
| spellingShingle | Ya-Ting Chang Chih-Ching Wu Yi-Ming Shyr Tse-Ching Chen Tsann-Long Hwang Ta-Sen Yeh Kai-Ping Chang Hao-Ping Liu Yu-Ling Liu Ming-Hung Tsai Yu-Sun Chang Jau-Song Yu Secretome-based identification of ULBP2 as a novel serum marker for pancreatic cancer detection. PLoS ONE |
| title | Secretome-based identification of ULBP2 as a novel serum marker for pancreatic cancer detection. |
| title_full | Secretome-based identification of ULBP2 as a novel serum marker for pancreatic cancer detection. |
| title_fullStr | Secretome-based identification of ULBP2 as a novel serum marker for pancreatic cancer detection. |
| title_full_unstemmed | Secretome-based identification of ULBP2 as a novel serum marker for pancreatic cancer detection. |
| title_short | Secretome-based identification of ULBP2 as a novel serum marker for pancreatic cancer detection. |
| title_sort | secretome based identification of ulbp2 as a novel serum marker for pancreatic cancer detection |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625447/pdf/?tool=EBI |
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