High Genetic Diversity and Low Differentiation in <i>Michelia shiluensis</i>, an Endangered Magnolia Species in South China
<i>Research Highlights</i>: This study is the first to examine the genetic diversity of <i>Michelia shiluensis</i> (Magnoliaceae). High genetic diversity and low differentiation were detected in this species. Based on these results, we discuss feasible protection measures to...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/11/4/469 |
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| author | Yanwen Deng Tingting Liu Yuqing Xie Yaqing Wei Zicai Xie Youhai Shi Xiaomei Deng |
| author_facet | Yanwen Deng Tingting Liu Yuqing Xie Yaqing Wei Zicai Xie Youhai Shi Xiaomei Deng |
| author_sort | Yanwen Deng |
| collection | DOAJ |
| description | <i>Research Highlights</i>: This study is the first to examine the genetic diversity of <i>Michelia shiluensis</i> (Magnoliaceae). High genetic diversity and low differentiation were detected in this species. Based on these results, we discuss feasible protection measures to provide a basis for the conservation and utilization of <i>M. shiluensis</i>. <i>Background and Objectives</i>: <i>Michelia shiluensis</i> is distributed in Hainan and Guangdong province, China. Due to human disturbance, the population has decreased sharply, and there is thus an urgent need to evaluate genetic variation within this species in order to identify an optimal conservation strategy. <i>Materials and Methods</i>: In this study, we used eight nuclear single sequence repeat (nSSR) markers and two chloroplast DNA (cpDNA) markers to assess the genetic diversity, population structure, and dynamics of 78 samples collected from six populations. <i>Results</i>: The results showed that the average observed heterozygosity (Ho), expected heterozygosity (He), and percentage of polymorphic loci (PPL) from nSSR markers in each population of <i>M. shiluensis</i> were 0.686, 0.718, and 97.92%, respectively. For cpDNA markers, the overall haplotype diversity (Hd) was 0.674, and the nucleotide diversity was 0.220. Analysis of markers showed that the genetic variation between populations was much lower based on nSSR than on cpDNA (10.18% and 77.56%, respectively, based on an analysis of molecular variance (AMOVA)). Analysis of the population structure based on the two markers shows that one of the populations (DL) is very different from the other five. <i>Conclusions</i>: High genetic diversity and low population differentiation of <i>M. shiluensis</i> might be the result of rich ancestral genetic variation. The current decline in population may therefore be due to human disturbance rather than to inbreeding or genetic drift. Management and conservation strategies should focus on maintaining the genetic diversity in situ, and on the cultivation of seedlings ex-situ for transplanting back to their original habitat. |
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| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-10T20:19:15Z |
| publishDate | 2020-04-01 |
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| spelling | doaj.art-5f81f57467e14bad96299fca27780e952023-11-19T22:17:18ZengMDPI AGForests1999-49072020-04-0111446910.3390/f11040469High Genetic Diversity and Low Differentiation in <i>Michelia shiluensis</i>, an Endangered Magnolia Species in South ChinaYanwen Deng0Tingting Liu1Yuqing Xie2Yaqing Wei3Zicai Xie4Youhai Shi5Xiaomei Deng6Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou 510642, ChinaGuangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou 510642, ChinaGuangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou 510642, ChinaKey Laboratory of Germplasm Resources Biology of Tropical Special Ornamental Plants of Hainan Province, Haikou 570228, ChinaCollege of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Germplasm Resources Biology of Tropical Special Ornamental Plants of Hainan Province, Haikou 570228, ChinaGuangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou 510642, China<i>Research Highlights</i>: This study is the first to examine the genetic diversity of <i>Michelia shiluensis</i> (Magnoliaceae). High genetic diversity and low differentiation were detected in this species. Based on these results, we discuss feasible protection measures to provide a basis for the conservation and utilization of <i>M. shiluensis</i>. <i>Background and Objectives</i>: <i>Michelia shiluensis</i> is distributed in Hainan and Guangdong province, China. Due to human disturbance, the population has decreased sharply, and there is thus an urgent need to evaluate genetic variation within this species in order to identify an optimal conservation strategy. <i>Materials and Methods</i>: In this study, we used eight nuclear single sequence repeat (nSSR) markers and two chloroplast DNA (cpDNA) markers to assess the genetic diversity, population structure, and dynamics of 78 samples collected from six populations. <i>Results</i>: The results showed that the average observed heterozygosity (Ho), expected heterozygosity (He), and percentage of polymorphic loci (PPL) from nSSR markers in each population of <i>M. shiluensis</i> were 0.686, 0.718, and 97.92%, respectively. For cpDNA markers, the overall haplotype diversity (Hd) was 0.674, and the nucleotide diversity was 0.220. Analysis of markers showed that the genetic variation between populations was much lower based on nSSR than on cpDNA (10.18% and 77.56%, respectively, based on an analysis of molecular variance (AMOVA)). Analysis of the population structure based on the two markers shows that one of the populations (DL) is very different from the other five. <i>Conclusions</i>: High genetic diversity and low population differentiation of <i>M. shiluensis</i> might be the result of rich ancestral genetic variation. The current decline in population may therefore be due to human disturbance rather than to inbreeding or genetic drift. Management and conservation strategies should focus on maintaining the genetic diversity in situ, and on the cultivation of seedlings ex-situ for transplanting back to their original habitat.https://www.mdpi.com/1999-4907/11/4/469nSSRcpDNAMagnoliaceaeconservation geneticsfragmentation |
| spellingShingle | Yanwen Deng Tingting Liu Yuqing Xie Yaqing Wei Zicai Xie Youhai Shi Xiaomei Deng High Genetic Diversity and Low Differentiation in <i>Michelia shiluensis</i>, an Endangered Magnolia Species in South China Forests nSSR cpDNA Magnoliaceae conservation genetics fragmentation |
| title | High Genetic Diversity and Low Differentiation in <i>Michelia shiluensis</i>, an Endangered Magnolia Species in South China |
| title_full | High Genetic Diversity and Low Differentiation in <i>Michelia shiluensis</i>, an Endangered Magnolia Species in South China |
| title_fullStr | High Genetic Diversity and Low Differentiation in <i>Michelia shiluensis</i>, an Endangered Magnolia Species in South China |
| title_full_unstemmed | High Genetic Diversity and Low Differentiation in <i>Michelia shiluensis</i>, an Endangered Magnolia Species in South China |
| title_short | High Genetic Diversity and Low Differentiation in <i>Michelia shiluensis</i>, an Endangered Magnolia Species in South China |
| title_sort | high genetic diversity and low differentiation in i michelia shiluensis i an endangered magnolia species in south china |
| topic | nSSR cpDNA Magnoliaceae conservation genetics fragmentation |
| url | https://www.mdpi.com/1999-4907/11/4/469 |
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