Possible hepatotoxic consequence of nevirapine use in juvenile albino rats
Context: Nevirapine (NVP) is used in human immunodeficiency virus exposed neonates. This could present safety concern due to decreased liver metabolizing enzymes activity and renal clearance in neonates. Aims: To determine the hepatotoxic effect of NVP in juvenile albino rats. Methods: Juvenil...
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| Format: | Article |
| Language: | English |
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GarVal Editorial Ltda.
2017-08-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | http://jppres.com/jppres/pdf/vol5/jppres16.196_5.4.217.pdf |
| _version_ | 1830201792754876416 |
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| author | Elias Adikwu Bonsome Bokolo |
| author_facet | Elias Adikwu Bonsome Bokolo |
| author_sort | Elias Adikwu |
| collection | DOAJ |
| description | Context: Nevirapine (NVP) is used in human immunodeficiency virus exposed neonates. This could present safety concern due to decreased liver metabolizing enzymes activity and renal clearance in neonates.
Aims: To determine the hepatotoxic effect of NVP in juvenile albino rats.
Methods: Juvenile albino rats were weighed, divided into groups and treated orally with 4-32 mg/kg/day of NVP for 14 days including a recovery group. The control groups were treated with water (placebo) and normal saline (solvent). At the end of NVP treatment, rats were weighed and sacrificed, blood was collected and serum extracted. Serum was analyzed for alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (TB) and conjugated bilirubin (CB). The liver was harvested via dissection, weighed and evaluated for AST, ALT, ALP, superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), malondialdehyde (MDA) levels and histological damage.
Results: The body, absolute and relative liver weights of rats in NVP treated groups were not significantly different (p>0.05) when compared to placebo. However, serum levels of AST, ALT, ALP, TB and CB were significantly increased (p<0.05) in a dose-dependent manner in NVP-treated groups. Furthermore, liver levels of ALT, ALP, AST and MDA were significantly increased (p<0.05) while SOD, CAT, and GSH were decreased in a dose dependent manner in NVP-treated groups. NVP-treated rats were characterized by varying degrees of hepatic morphological alterations. However, in the recovery group, the effects of NVP were reversed.
Conclusions: This study observed dose-dependent and reversible hepatotoxicity in nevirapine- treated juvenile albino rats. |
| first_indexed | 2024-12-18T02:58:55Z |
| format | Article |
| id | doaj.art-5f825666107a4e5e905e7d9bc56dae8e |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-12-18T02:58:55Z |
| publishDate | 2017-08-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-5f825666107a4e5e905e7d9bc56dae8e2022-12-21T21:23:18ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502017-08-0154217226Possible hepatotoxic consequence of nevirapine use in juvenile albino ratsElias Adikwu0Bonsome Bokolo1Department of Pharmacology, Faculty of Basic Medical Sciences, University of Port Harcourt, Choba, Rivers State, NigeriaDepartment of Pharmacology, Faculty of Basic Medical Sciences, Niger Delta University Wilberforce Island, Bayelsa State, NigeriaContext: Nevirapine (NVP) is used in human immunodeficiency virus exposed neonates. This could present safety concern due to decreased liver metabolizing enzymes activity and renal clearance in neonates. Aims: To determine the hepatotoxic effect of NVP in juvenile albino rats. Methods: Juvenile albino rats were weighed, divided into groups and treated orally with 4-32 mg/kg/day of NVP for 14 days including a recovery group. The control groups were treated with water (placebo) and normal saline (solvent). At the end of NVP treatment, rats were weighed and sacrificed, blood was collected and serum extracted. Serum was analyzed for alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (TB) and conjugated bilirubin (CB). The liver was harvested via dissection, weighed and evaluated for AST, ALT, ALP, superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), malondialdehyde (MDA) levels and histological damage. Results: The body, absolute and relative liver weights of rats in NVP treated groups were not significantly different (p>0.05) when compared to placebo. However, serum levels of AST, ALT, ALP, TB and CB were significantly increased (p<0.05) in a dose-dependent manner in NVP-treated groups. Furthermore, liver levels of ALT, ALP, AST and MDA were significantly increased (p<0.05) while SOD, CAT, and GSH were decreased in a dose dependent manner in NVP-treated groups. NVP-treated rats were characterized by varying degrees of hepatic morphological alterations. However, in the recovery group, the effects of NVP were reversed. Conclusions: This study observed dose-dependent and reversible hepatotoxicity in nevirapine- treated juvenile albino rats.http://jppres.com/jppres/pdf/vol5/jppres16.196_5.4.217.pdfjuvenile ratslivernevirapineoxidative stresstoxicity |
| spellingShingle | Elias Adikwu Bonsome Bokolo Possible hepatotoxic consequence of nevirapine use in juvenile albino rats Journal of Pharmacy & Pharmacognosy Research juvenile rats liver nevirapine oxidative stress toxicity |
| title | Possible hepatotoxic consequence of nevirapine use in juvenile albino rats |
| title_full | Possible hepatotoxic consequence of nevirapine use in juvenile albino rats |
| title_fullStr | Possible hepatotoxic consequence of nevirapine use in juvenile albino rats |
| title_full_unstemmed | Possible hepatotoxic consequence of nevirapine use in juvenile albino rats |
| title_short | Possible hepatotoxic consequence of nevirapine use in juvenile albino rats |
| title_sort | possible hepatotoxic consequence of nevirapine use in juvenile albino rats |
| topic | juvenile rats liver nevirapine oxidative stress toxicity |
| url | http://jppres.com/jppres/pdf/vol5/jppres16.196_5.4.217.pdf |
| work_keys_str_mv | AT eliasadikwu possiblehepatotoxicconsequenceofnevirapineuseinjuvenilealbinorats AT bonsomebokolo possiblehepatotoxicconsequenceofnevirapineuseinjuvenilealbinorats |