Subtyping on Live Lymphoma Cell Lines by Raman Spectroscopy
Raman spectroscopy is a well-defined spectroscopic technique sensitive to the molecular vibrations of materials, since it provides fingerprint-like information regarding the molecular structure of the analyzed samples. It has been extensively used for non-destructive and label-free cell characteriza...
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MDPI AG
2022-01-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/15/2/546 |
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| author | Klytaimnistra Katsara Konstantina Psatha George Kenanakis Michalis Aivaliotis Vassilis M. Papadakis |
| author_facet | Klytaimnistra Katsara Konstantina Psatha George Kenanakis Michalis Aivaliotis Vassilis M. Papadakis |
| author_sort | Klytaimnistra Katsara |
| collection | DOAJ |
| description | Raman spectroscopy is a well-defined spectroscopic technique sensitive to the molecular vibrations of materials, since it provides fingerprint-like information regarding the molecular structure of the analyzed samples. It has been extensively used for non-destructive and label-free cell characterization, particularly in the qualitative and quantitative estimation of amino acids, lipids, nucleic acids, and carbohydrates. Lymphoma cell classification is a crucial task for accurate and prompt lymphoma diagnosis, prognosis, and treatment. Currently, it is mostly based on limited information and requires costly and time-consuming approaches. In this work, we are proposing a fast characterization and differentiation methodology of lymphoma cell subtypes based on Raman spectroscopy. The study was performed in the temperature range of 15–37 °C to identify the best cell measurement conditions. The proposed methodology is fast, accurate, and requires minimal sample preparation, resulting in a potentially promising, non-invasive strategy for early and accurate cell lymphoma characterization. |
| first_indexed | 2024-03-10T01:03:32Z |
| format | Article |
| id | doaj.art-5f83fe7829184589a70e3d865847c270 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T01:03:32Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-5f83fe7829184589a70e3d865847c2702023-11-23T14:30:59ZengMDPI AGMaterials1996-19442022-01-0115254610.3390/ma15020546Subtyping on Live Lymphoma Cell Lines by Raman SpectroscopyKlytaimnistra Katsara0Konstantina Psatha1George Kenanakis2Michalis Aivaliotis3Vassilis M. Papadakis4Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, N. Plastira 100, GR-70013 Heraklion, GreeceInstitute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, N. Plastira 100, GR-70013 Heraklion, GreeceInstitute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas, N. Plastira 100, GR-70013 Heraklion, GreeceInstitute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, N. Plastira 100, GR-70013 Heraklion, GreeceInstitute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, N. Plastira 100, GR-70013 Heraklion, GreeceRaman spectroscopy is a well-defined spectroscopic technique sensitive to the molecular vibrations of materials, since it provides fingerprint-like information regarding the molecular structure of the analyzed samples. It has been extensively used for non-destructive and label-free cell characterization, particularly in the qualitative and quantitative estimation of amino acids, lipids, nucleic acids, and carbohydrates. Lymphoma cell classification is a crucial task for accurate and prompt lymphoma diagnosis, prognosis, and treatment. Currently, it is mostly based on limited information and requires costly and time-consuming approaches. In this work, we are proposing a fast characterization and differentiation methodology of lymphoma cell subtypes based on Raman spectroscopy. The study was performed in the temperature range of 15–37 °C to identify the best cell measurement conditions. The proposed methodology is fast, accurate, and requires minimal sample preparation, resulting in a potentially promising, non-invasive strategy for early and accurate cell lymphoma characterization.https://www.mdpi.com/1996-1944/15/2/546Raman spectroscopyPCAsubtypingcell linesnon-Hodgkin lymphomaHodgkin lymphoma |
| spellingShingle | Klytaimnistra Katsara Konstantina Psatha George Kenanakis Michalis Aivaliotis Vassilis M. Papadakis Subtyping on Live Lymphoma Cell Lines by Raman Spectroscopy Materials Raman spectroscopy PCA subtyping cell lines non-Hodgkin lymphoma Hodgkin lymphoma |
| title | Subtyping on Live Lymphoma Cell Lines by Raman Spectroscopy |
| title_full | Subtyping on Live Lymphoma Cell Lines by Raman Spectroscopy |
| title_fullStr | Subtyping on Live Lymphoma Cell Lines by Raman Spectroscopy |
| title_full_unstemmed | Subtyping on Live Lymphoma Cell Lines by Raman Spectroscopy |
| title_short | Subtyping on Live Lymphoma Cell Lines by Raman Spectroscopy |
| title_sort | subtyping on live lymphoma cell lines by raman spectroscopy |
| topic | Raman spectroscopy PCA subtyping cell lines non-Hodgkin lymphoma Hodgkin lymphoma |
| url | https://www.mdpi.com/1996-1944/15/2/546 |
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