Methods, development and applications of small-angle X-ray scattering to characterize biological macromolecules in solution
Applications of small-angle X-ray scattering (SAXS) in structural biology are reviewed. A brief introduction of the SAXS basics is followed by the presentation of the structural features of biological macromolecules in solution that can be assessed by SAXS. The approaches are considered allowing one...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-01-01
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| Series: | Current Research in Structural Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665928X20300180 |
| _version_ | 1818448353480933376 |
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| author | Stefano Da Vela Dmitri I. Svergun |
| author_facet | Stefano Da Vela Dmitri I. Svergun |
| author_sort | Stefano Da Vela |
| collection | DOAJ |
| description | Applications of small-angle X-ray scattering (SAXS) in structural biology are reviewed. A brief introduction of the SAXS basics is followed by the presentation of the structural features of biological macromolecules in solution that can be assessed by SAXS. The approaches are considered allowing one to obtain low resolution three-dimensional (3D) structural models and to describe assembly states and conformations. Metrics and descriptors required for the assessment of model quality are presented and recent biological applications of SAXS are shown. |
| first_indexed | 2024-12-14T20:18:10Z |
| format | Article |
| id | doaj.art-5f84322a22da48ae956f174846aaee46 |
| institution | Directory Open Access Journal |
| issn | 2665-928X |
| language | English |
| last_indexed | 2024-12-14T20:18:10Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Structural Biology |
| spelling | doaj.art-5f84322a22da48ae956f174846aaee462022-12-21T22:48:48ZengElsevierCurrent Research in Structural Biology2665-928X2020-01-012164170Methods, development and applications of small-angle X-ray scattering to characterize biological macromolecules in solutionStefano Da Vela0Dmitri I. Svergun1Corresponding author.; EMBL Hamburg Unit, c/o DESY, Notkestr. 85, 22607, Hamburg, GermanyEMBL Hamburg Unit, c/o DESY, Notkestr. 85, 22607, Hamburg, GermanyApplications of small-angle X-ray scattering (SAXS) in structural biology are reviewed. A brief introduction of the SAXS basics is followed by the presentation of the structural features of biological macromolecules in solution that can be assessed by SAXS. The approaches are considered allowing one to obtain low resolution three-dimensional (3D) structural models and to describe assembly states and conformations. Metrics and descriptors required for the assessment of model quality are presented and recent biological applications of SAXS are shown.http://www.sciencedirect.com/science/article/pii/S2665928X20300180SAXSStructural biologyProteinsComplexesBiophysics |
| spellingShingle | Stefano Da Vela Dmitri I. Svergun Methods, development and applications of small-angle X-ray scattering to characterize biological macromolecules in solution Current Research in Structural Biology SAXS Structural biology Proteins Complexes Biophysics |
| title | Methods, development and applications of small-angle X-ray scattering to characterize biological macromolecules in solution |
| title_full | Methods, development and applications of small-angle X-ray scattering to characterize biological macromolecules in solution |
| title_fullStr | Methods, development and applications of small-angle X-ray scattering to characterize biological macromolecules in solution |
| title_full_unstemmed | Methods, development and applications of small-angle X-ray scattering to characterize biological macromolecules in solution |
| title_short | Methods, development and applications of small-angle X-ray scattering to characterize biological macromolecules in solution |
| title_sort | methods development and applications of small angle x ray scattering to characterize biological macromolecules in solution |
| topic | SAXS Structural biology Proteins Complexes Biophysics |
| url | http://www.sciencedirect.com/science/article/pii/S2665928X20300180 |
| work_keys_str_mv | AT stefanodavela methodsdevelopmentandapplicationsofsmallanglexrayscatteringtocharacterizebiologicalmacromoleculesinsolution AT dmitriisvergun methodsdevelopmentandapplicationsofsmallanglexrayscatteringtocharacterizebiologicalmacromoleculesinsolution |