The Examination of mecA gene in Methicillin-Resistant Staphylococcus aureus (MRSA) and inappropriate antibiotic uses of healthcare workers and communities in Banyumas
Background: Methicillin-Resistant Staphylococcus aureus (MRSA) has become a major pathogene and its incidence continues to increase in various countries. The resistance to methicillin is caused by the mecA gene that encodes the expression of Penicillin Binding Protein 2a (PBP2a) so it has a low affi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Medicine
2020-12-01
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| Series: | JKKI (Jurnal Kedokteran dan Kesehatan Indonesia) |
| Subjects: | |
| Online Access: | https://journal.uii.ac.id/JKKI/article/view/13736 |
| _version_ | 1827906197765750784 |
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| author | Metta Ayu Susanti Gembong Satria Mahardhika Lantip Rujito Anton Budhi Darmawan Dwi Utami Anjarwati |
| author_facet | Metta Ayu Susanti Gembong Satria Mahardhika Lantip Rujito Anton Budhi Darmawan Dwi Utami Anjarwati |
| author_sort | Metta Ayu Susanti |
| collection | DOAJ |
| description | Background: Methicillin-Resistant Staphylococcus aureus (MRSA) has become a major pathogene and its incidence continues to increase in various countries. The resistance to methicillin is caused by the mecA gene that encodes the expression of Penicillin Binding Protein 2a (PBP2a) so it has a low affinity for beta-lactam antibiotics. One of the factors that can increase the incidence of MRSA is inappropriate antibiotics use.
Objective: This study is to identify presence of the mecA gene in MRSA and to compare histories of inappropriate antibiotic uses of healthcare workers and communities in Banyumas.
Methods: This was a cross-sectional study conducting 120 samples from nasal swabs. Isolates of MRSA tested by bacteriological examinations and PCR of the mecA genes. Histories of antibiotic uses were assessed by using questionnaires and then analysed descriptively by using Fisher Exact test (SPPS, version 20).
Result: One sample of S. aureus from the microbiology examination (0.83%) was an MRSA although there was no a mecA gene identified by using the PCR method. 66.1% of the healthcare workers and 88,3% of the communities used antibiotics inappropriately. The results showed that there was a statistically significant difference in the histories of irrational uses of antibiotics (p?0.05).
Conclusion: A number of the communities who used inappropriate antibiotics was higher than the health workers. Furthermore, one MRSA strain with a negative mecA gene was found in the health worker group. |
| first_indexed | 2024-03-13T00:54:08Z |
| format | Article |
| id | doaj.art-5f84d454af8043918d48c3762e9e6346 |
| institution | Directory Open Access Journal |
| issn | 2085-4145 2527-2950 |
| language | English |
| last_indexed | 2024-03-13T00:54:08Z |
| publishDate | 2020-12-01 |
| publisher | Faculty of Medicine |
| record_format | Article |
| series | JKKI (Jurnal Kedokteran dan Kesehatan Indonesia) |
| spelling | doaj.art-5f84d454af8043918d48c3762e9e63462023-07-07T07:41:36ZengFaculty of MedicineJKKI (Jurnal Kedokteran dan Kesehatan Indonesia)2085-41452527-29502020-12-0125726510.20885/JKKI.Vol11.Iss3.art711217The Examination of mecA gene in Methicillin-Resistant Staphylococcus aureus (MRSA) and inappropriate antibiotic uses of healthcare workers and communities in BanyumasMetta Ayu Susanti0https://orcid.org/0000-0003-0720-4375Gembong Satria Mahardhika1https://orcid.org/0000-0003-3996-2517Lantip Rujito2Anton Budhi Darmawan3https://orcid.org/0000-0002-3298-0272Dwi Utami Anjarwati4https://orcid.org/0000-0001-8394-2543Biomedical Master’s Program, Faculty of Medicine, Universitas Jenderal SoedirmanBiomedical Master’s Program, Faculty of Medicine, Universitas Jenderal SoedirmanClinical Genetic of Anatomy Department, Faculty of Medicine, Universitas Jenderal Soedirman, PurwokertoDepartment of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, Universitas Jenderal Soedirman-Margono Soekarjo Hospital, PurwokertoMicrobiology Department, Faculty of Medicine, Universitas Jenderal SoedirmanBackground: Methicillin-Resistant Staphylococcus aureus (MRSA) has become a major pathogene and its incidence continues to increase in various countries. The resistance to methicillin is caused by the mecA gene that encodes the expression of Penicillin Binding Protein 2a (PBP2a) so it has a low affinity for beta-lactam antibiotics. One of the factors that can increase the incidence of MRSA is inappropriate antibiotics use. Objective: This study is to identify presence of the mecA gene in MRSA and to compare histories of inappropriate antibiotic uses of healthcare workers and communities in Banyumas. Methods: This was a cross-sectional study conducting 120 samples from nasal swabs. Isolates of MRSA tested by bacteriological examinations and PCR of the mecA genes. Histories of antibiotic uses were assessed by using questionnaires and then analysed descriptively by using Fisher Exact test (SPPS, version 20). Result: One sample of S. aureus from the microbiology examination (0.83%) was an MRSA although there was no a mecA gene identified by using the PCR method. 66.1% of the healthcare workers and 88,3% of the communities used antibiotics inappropriately. The results showed that there was a statistically significant difference in the histories of irrational uses of antibiotics (p?0.05). Conclusion: A number of the communities who used inappropriate antibiotics was higher than the health workers. Furthermore, one MRSA strain with a negative mecA gene was found in the health worker group.https://journal.uii.ac.id/JKKI/article/view/13736antibioticsmrsahealthcare workerscommunity |
| spellingShingle | Metta Ayu Susanti Gembong Satria Mahardhika Lantip Rujito Anton Budhi Darmawan Dwi Utami Anjarwati The Examination of mecA gene in Methicillin-Resistant Staphylococcus aureus (MRSA) and inappropriate antibiotic uses of healthcare workers and communities in Banyumas JKKI (Jurnal Kedokteran dan Kesehatan Indonesia) antibiotics mrsa healthcare workers community |
| title | The Examination of mecA gene in Methicillin-Resistant Staphylococcus aureus (MRSA) and inappropriate antibiotic uses of healthcare workers and communities in Banyumas |
| title_full | The Examination of mecA gene in Methicillin-Resistant Staphylococcus aureus (MRSA) and inappropriate antibiotic uses of healthcare workers and communities in Banyumas |
| title_fullStr | The Examination of mecA gene in Methicillin-Resistant Staphylococcus aureus (MRSA) and inappropriate antibiotic uses of healthcare workers and communities in Banyumas |
| title_full_unstemmed | The Examination of mecA gene in Methicillin-Resistant Staphylococcus aureus (MRSA) and inappropriate antibiotic uses of healthcare workers and communities in Banyumas |
| title_short | The Examination of mecA gene in Methicillin-Resistant Staphylococcus aureus (MRSA) and inappropriate antibiotic uses of healthcare workers and communities in Banyumas |
| title_sort | examination of meca gene in methicillin resistant staphylococcus aureus mrsa and inappropriate antibiotic uses of healthcare workers and communities in banyumas |
| topic | antibiotics mrsa healthcare workers community |
| url | https://journal.uii.ac.id/JKKI/article/view/13736 |
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