Overexpression of <i>ApHIPP26</i> from the Hyperaccumulator <i>Arabis paniculata</i> Confers Enhanced Cadmium Tolerance and Accumulation to <i>Arabidopsis thaliana</i>
Cadmium (Cd) is a toxic heavy metal that seriously affects metabolism after accumulation in plants, and it also causes adverse effects on humans through the food chain. The HIPP gene family has been shown to be highly tolerant to Cd stress due to its special domain and molecular structure. This stud...
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| author | Lizhou Zhou Lvlan Ye Biao Pang Yunyan Hou Junxing Yu Xuye Du Lei Gu Hongcheng Wang Bin Zhu |
| author_facet | Lizhou Zhou Lvlan Ye Biao Pang Yunyan Hou Junxing Yu Xuye Du Lei Gu Hongcheng Wang Bin Zhu |
| author_sort | Lizhou Zhou |
| collection | DOAJ |
| description | Cadmium (Cd) is a toxic heavy metal that seriously affects metabolism after accumulation in plants, and it also causes adverse effects on humans through the food chain. The HIPP gene family has been shown to be highly tolerant to Cd stress due to its special domain and molecular structure. This study described the Cd-induced gene <i>ApHIPP26</i> from the hyperaccumulator <i>Arabis paniculata</i>. Its subcellular localization showed that ApHIPP26 was located in the nucleus. Transgenic <i>Arabidopsis</i> overexpressing <i>ApHIPP26</i> exhibited a significant increase in main root length and fresh weight under Cd stress. Compared with wild-type lines, Cd accumulated much more in transgenic <i>Arabidopsis</i> both aboveground and underground. Under Cd stress, the expression of genes related to the absorption and transport of heavy metals underwent different changes in parallel, which were involved in the accumulation and distribution of Cd in plants, such as <i>AtNRAMP6</i> and <i>AtNRAMP3</i>. Under Cd stress, the activities of antioxidant enzymes (superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase) in the transgenic lines were higher than those in the wild type. The physiological and biochemical indices showed that the proline and chlorophyll contents in the transgenic lines increased significantly after Cd treatment, while the malondialdehyde (MDA) content decreased. In addition, the gene expression profile analysis showed that <i>ApHIPP26</i> improved the tolerance of <i>Arabidopsis</i> to Cd by regulating the changes of related genes in plant hormone signal transduction pathway. In conclusion, <i>ApHIPP26</i> plays an important role in cadmium tolerance by alleviating oxidative stress and regulating plant hormones, which provides a basis for understanding the molecular mechanism of cadmium tolerance in plants and provides new insights for phytoremediation in Cd-contaminated areas. |
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| language | English |
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| spelling | doaj.art-5f857d47cc61499695a9fb1d133bf0f82023-11-19T16:40:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124201505210.3390/ijms242015052Overexpression of <i>ApHIPP26</i> from the Hyperaccumulator <i>Arabis paniculata</i> Confers Enhanced Cadmium Tolerance and Accumulation to <i>Arabidopsis thaliana</i>Lizhou Zhou0Lvlan Ye1Biao Pang2Yunyan Hou3Junxing Yu4Xuye Du5Lei Gu6Hongcheng Wang7Bin Zhu8School of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaSchool of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaSchool of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaSchool of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaSchool of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaSchool of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaSchool of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaSchool of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaSchool of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaCadmium (Cd) is a toxic heavy metal that seriously affects metabolism after accumulation in plants, and it also causes adverse effects on humans through the food chain. The HIPP gene family has been shown to be highly tolerant to Cd stress due to its special domain and molecular structure. This study described the Cd-induced gene <i>ApHIPP26</i> from the hyperaccumulator <i>Arabis paniculata</i>. Its subcellular localization showed that ApHIPP26 was located in the nucleus. Transgenic <i>Arabidopsis</i> overexpressing <i>ApHIPP26</i> exhibited a significant increase in main root length and fresh weight under Cd stress. Compared with wild-type lines, Cd accumulated much more in transgenic <i>Arabidopsis</i> both aboveground and underground. Under Cd stress, the expression of genes related to the absorption and transport of heavy metals underwent different changes in parallel, which were involved in the accumulation and distribution of Cd in plants, such as <i>AtNRAMP6</i> and <i>AtNRAMP3</i>. Under Cd stress, the activities of antioxidant enzymes (superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase) in the transgenic lines were higher than those in the wild type. The physiological and biochemical indices showed that the proline and chlorophyll contents in the transgenic lines increased significantly after Cd treatment, while the malondialdehyde (MDA) content decreased. In addition, the gene expression profile analysis showed that <i>ApHIPP26</i> improved the tolerance of <i>Arabidopsis</i> to Cd by regulating the changes of related genes in plant hormone signal transduction pathway. In conclusion, <i>ApHIPP26</i> plays an important role in cadmium tolerance by alleviating oxidative stress and regulating plant hormones, which provides a basis for understanding the molecular mechanism of cadmium tolerance in plants and provides new insights for phytoremediation in Cd-contaminated areas.https://www.mdpi.com/1422-0067/24/20/15052Cadmium stress<i>Arabis paniculata</i><i>ApHIPP26</i>transcriptomephytohormone pathway |
| spellingShingle | Lizhou Zhou Lvlan Ye Biao Pang Yunyan Hou Junxing Yu Xuye Du Lei Gu Hongcheng Wang Bin Zhu Overexpression of <i>ApHIPP26</i> from the Hyperaccumulator <i>Arabis paniculata</i> Confers Enhanced Cadmium Tolerance and Accumulation to <i>Arabidopsis thaliana</i> International Journal of Molecular Sciences Cadmium stress <i>Arabis paniculata</i> <i>ApHIPP26</i> transcriptome phytohormone pathway |
| title | Overexpression of <i>ApHIPP26</i> from the Hyperaccumulator <i>Arabis paniculata</i> Confers Enhanced Cadmium Tolerance and Accumulation to <i>Arabidopsis thaliana</i> |
| title_full | Overexpression of <i>ApHIPP26</i> from the Hyperaccumulator <i>Arabis paniculata</i> Confers Enhanced Cadmium Tolerance and Accumulation to <i>Arabidopsis thaliana</i> |
| title_fullStr | Overexpression of <i>ApHIPP26</i> from the Hyperaccumulator <i>Arabis paniculata</i> Confers Enhanced Cadmium Tolerance and Accumulation to <i>Arabidopsis thaliana</i> |
| title_full_unstemmed | Overexpression of <i>ApHIPP26</i> from the Hyperaccumulator <i>Arabis paniculata</i> Confers Enhanced Cadmium Tolerance and Accumulation to <i>Arabidopsis thaliana</i> |
| title_short | Overexpression of <i>ApHIPP26</i> from the Hyperaccumulator <i>Arabis paniculata</i> Confers Enhanced Cadmium Tolerance and Accumulation to <i>Arabidopsis thaliana</i> |
| title_sort | overexpression of i aphipp26 i from the hyperaccumulator i arabis paniculata i confers enhanced cadmium tolerance and accumulation to i arabidopsis thaliana i |
| topic | Cadmium stress <i>Arabis paniculata</i> <i>ApHIPP26</i> transcriptome phytohormone pathway |
| url | https://www.mdpi.com/1422-0067/24/20/15052 |
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