Clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Paraná, Brazil
The aim of the present work was to study the clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Parana, Brazil. Twenty one suspected cases of mucocutaneous leishmaniasis (MCL) in patients from the endemic areas of leishmaniasis were assessed. Differ...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2011-06-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300008 |
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| author | Andréa Thomaz-Soccol Marcos Mocellin Fabiane Mulinari Edilene Alcântara de Castro Flávio de Queiroz-Telles Fernanda de Souza Alcântara Marcello Tortelli Bavaresco Luciane Hennig Anielo Andraus Ennio Luz Vanete Thomaz-Soccol |
| author_facet | Andréa Thomaz-Soccol Marcos Mocellin Fabiane Mulinari Edilene Alcântara de Castro Flávio de Queiroz-Telles Fernanda de Souza Alcântara Marcello Tortelli Bavaresco Luciane Hennig Anielo Andraus Ennio Luz Vanete Thomaz-Soccol |
| author_sort | Andréa Thomaz-Soccol |
| collection | DOAJ |
| description | The aim of the present work was to study the clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Parana, Brazil. Twenty one suspected cases of mucocutaneous leishmaniasis (MCL) in patients from the endemic areas of leishmaniasis were assessed. Different methods used in diagnosing the disease and the polymerase chain reaction (PCR) technique were compared in order to establish the sensitivity of each method. Out of the 21 patients analyzed, 14.3% presented other etiologies such as vasculitis, syphilis, and paracoccidioidomycosis, with all tests negative for leishmaniasis. Out of the remaining 15 patients, 6.7% cases were confirmed for leishmaniasis by direct examination; 46.67% were positive for culture, which allowed isolating and identifying the parasite and - with the PCR technique - it was possible to diagnose 100% MCL patients for all the three repetitions of exams. The PCR optimized for the present work proved to be an auxiliary method for diagnosing leishmaniasis applicable in the patients carrying MCL due to Leishmania (Viannia) braziliensis and did not need culture to be performed, resulting in a faster diagnosis. |
| first_indexed | 2024-12-12T03:40:40Z |
| format | Article |
| id | doaj.art-5f85bea19af346fcbb792e6e5acd9609 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-12T03:40:40Z |
| publishDate | 2011-06-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-5f85bea19af346fcbb792e6e5acd96092022-12-22T00:39:41ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242011-06-0154348749410.1590/S1516-89132011000300008Clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Paraná, BrazilAndréa Thomaz-SoccolMarcos MocellinFabiane MulinariEdilene Alcântara de CastroFlávio de Queiroz-TellesFernanda de Souza AlcântaraMarcello Tortelli BavarescoLuciane HennigAnielo AndrausEnnio LuzVanete Thomaz-SoccolThe aim of the present work was to study the clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Parana, Brazil. Twenty one suspected cases of mucocutaneous leishmaniasis (MCL) in patients from the endemic areas of leishmaniasis were assessed. Different methods used in diagnosing the disease and the polymerase chain reaction (PCR) technique were compared in order to establish the sensitivity of each method. Out of the 21 patients analyzed, 14.3% presented other etiologies such as vasculitis, syphilis, and paracoccidioidomycosis, with all tests negative for leishmaniasis. Out of the remaining 15 patients, 6.7% cases were confirmed for leishmaniasis by direct examination; 46.67% were positive for culture, which allowed isolating and identifying the parasite and - with the PCR technique - it was possible to diagnose 100% MCL patients for all the three repetitions of exams. The PCR optimized for the present work proved to be an auxiliary method for diagnosing leishmaniasis applicable in the patients carrying MCL due to Leishmania (Viannia) braziliensis and did not need culture to be performed, resulting in a faster diagnosis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300008Mucocutaneous leishmaniasismolecular diagnosisLeishmania (Viannia) braziliensis |
| spellingShingle | Andréa Thomaz-Soccol Marcos Mocellin Fabiane Mulinari Edilene Alcântara de Castro Flávio de Queiroz-Telles Fernanda de Souza Alcântara Marcello Tortelli Bavaresco Luciane Hennig Anielo Andraus Ennio Luz Vanete Thomaz-Soccol Clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Paraná, Brazil Brazilian Archives of Biology and Technology Mucocutaneous leishmaniasis molecular diagnosis Leishmania (Viannia) braziliensis |
| title | Clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Paraná, Brazil |
| title_full | Clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Paraná, Brazil |
| title_fullStr | Clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Paraná, Brazil |
| title_full_unstemmed | Clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Paraná, Brazil |
| title_short | Clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Paraná, Brazil |
| title_sort | clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in parana brazil |
| topic | Mucocutaneous leishmaniasis molecular diagnosis Leishmania (Viannia) braziliensis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300008 |
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