MEIS1 promotes expression of stem cell markers in esophageal squamous cell carcinoma
Abstract Background MEIS1 (Myeloid ecotropic viral integration site 1) as a homeobox (HOX) transcription factor plays regulatory roles in a variety of cellular processes including development, differentiation, survival, apoptosis and hematopoiesis, as well as stem cell regulation. Few studies have e...
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| Format: | Article |
| Language: | English |
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BMC
2020-08-01
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| Series: | BMC Cancer |
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| Online Access: | http://link.springer.com/article/10.1186/s12885-020-07307-0 |
| _version_ | 1818148688420143104 |
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| author | Selma Zargari Shabnam Negahban Khameneh Abolfazl Rad Mohammad Mahdi Forghanifard |
| author_facet | Selma Zargari Shabnam Negahban Khameneh Abolfazl Rad Mohammad Mahdi Forghanifard |
| author_sort | Selma Zargari |
| collection | DOAJ |
| description | Abstract Background MEIS1 (Myeloid ecotropic viral integration site 1) as a homeobox (HOX) transcription factor plays regulatory roles in a variety of cellular processes including development, differentiation, survival, apoptosis and hematopoiesis, as well as stem cell regulation. Few studies have established pluripotency and self-renewal regulatory roles for MEIS1 in human esophageal squamous cell carcinoma (ESCC), and our aim in this study was to evaluate the functional correlation between MEIS1 and the stemness markers in ESCC patients and cell line KYSE-30. Methods Expression pattern of MEIS1 and SALL4 gene expression was analyzed in different pathological features of ESCC patients. shRNA in retroviral vector was used for constantly silencing of MEIS1 mRNA in ESCC line (KYSE-30). Knockdown of MEIS1 gene and the expression pattern of selected stemness markers including SALL4, OCT4, BMI-1, HIWI, NANOG, PLK1, and KLF4 were evaluated using real-time PCR. Results Significant correlations were observed between MEIS1 and stemness marker SALL4 in different early pathological features of ESCC including non-invaded tumors, and the tumors with primary stages of progression. Retroviral knockdown of MEIS1 in KYSE-30 cells caused a noteworthy underexpression of both MEIS1 and major involved markers in stemness state of the cells including SALL4, OCT4, BMI-1, HIWI and KLF4. Conclusions The results highlight the important potential role of MEIS1 in modulating stemness properties of ESCCs and cells KYSE-30. These findings may confirm the linkage between MEIS1 and self-renewal capacity in ESCC and support probable oncogenic role for MEIS1 in the disease. |
| first_indexed | 2024-12-11T12:55:07Z |
| format | Article |
| id | doaj.art-5f880262a4e64090be5c0f524483da28 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-11T12:55:07Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-5f880262a4e64090be5c0f524483da282022-12-22T01:06:36ZengBMCBMC Cancer1471-24072020-08-012011810.1186/s12885-020-07307-0MEIS1 promotes expression of stem cell markers in esophageal squamous cell carcinomaSelma Zargari0Shabnam Negahban Khameneh1Abolfazl Rad2Mohammad Mahdi Forghanifard3Medical Genetics Research Center, Mashhad University of Medical SciencesDepartment of Biology, Damghan branch, Islamic Azad UniversityCellular and Molecular Research center, Sabzevar University of Medical SciencesDepartment of Biology, Damghan branch, Islamic Azad UniversityAbstract Background MEIS1 (Myeloid ecotropic viral integration site 1) as a homeobox (HOX) transcription factor plays regulatory roles in a variety of cellular processes including development, differentiation, survival, apoptosis and hematopoiesis, as well as stem cell regulation. Few studies have established pluripotency and self-renewal regulatory roles for MEIS1 in human esophageal squamous cell carcinoma (ESCC), and our aim in this study was to evaluate the functional correlation between MEIS1 and the stemness markers in ESCC patients and cell line KYSE-30. Methods Expression pattern of MEIS1 and SALL4 gene expression was analyzed in different pathological features of ESCC patients. shRNA in retroviral vector was used for constantly silencing of MEIS1 mRNA in ESCC line (KYSE-30). Knockdown of MEIS1 gene and the expression pattern of selected stemness markers including SALL4, OCT4, BMI-1, HIWI, NANOG, PLK1, and KLF4 were evaluated using real-time PCR. Results Significant correlations were observed between MEIS1 and stemness marker SALL4 in different early pathological features of ESCC including non-invaded tumors, and the tumors with primary stages of progression. Retroviral knockdown of MEIS1 in KYSE-30 cells caused a noteworthy underexpression of both MEIS1 and major involved markers in stemness state of the cells including SALL4, OCT4, BMI-1, HIWI and KLF4. Conclusions The results highlight the important potential role of MEIS1 in modulating stemness properties of ESCCs and cells KYSE-30. These findings may confirm the linkage between MEIS1 and self-renewal capacity in ESCC and support probable oncogenic role for MEIS1 in the disease.http://link.springer.com/article/10.1186/s12885-020-07307-0Self-renewalESCCKYSE-30MEIS1Stemness markers |
| spellingShingle | Selma Zargari Shabnam Negahban Khameneh Abolfazl Rad Mohammad Mahdi Forghanifard MEIS1 promotes expression of stem cell markers in esophageal squamous cell carcinoma BMC Cancer Self-renewal ESCC KYSE-30 MEIS1 Stemness markers |
| title | MEIS1 promotes expression of stem cell markers in esophageal squamous cell carcinoma |
| title_full | MEIS1 promotes expression of stem cell markers in esophageal squamous cell carcinoma |
| title_fullStr | MEIS1 promotes expression of stem cell markers in esophageal squamous cell carcinoma |
| title_full_unstemmed | MEIS1 promotes expression of stem cell markers in esophageal squamous cell carcinoma |
| title_short | MEIS1 promotes expression of stem cell markers in esophageal squamous cell carcinoma |
| title_sort | meis1 promotes expression of stem cell markers in esophageal squamous cell carcinoma |
| topic | Self-renewal ESCC KYSE-30 MEIS1 Stemness markers |
| url | http://link.springer.com/article/10.1186/s12885-020-07307-0 |
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