Expression, Purification and Bioactivities Analysis of Recombinant Active Peptide from Shark Liver
The Active Peptide from Shark Liver (APSL) was expressed in E. coli BL21 cells. The cDNA encoding APSL protein was obtained from shark regenerated hepatic tissue by RT-PCR, then it was cloned in the pET-28a expression vector. The expressed fusion protein was purified by Ni-IDA affinity chromatograph...
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| Format: | Article |
| Language: | English |
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MDPI AG
2009-06-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/7/2/258/ |
| _version_ | 1818012254163959808 |
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| author | Boping Ye Wutong Wu Qian Li Wenping Zhang Zhengbing Lv Yu Ou |
| author_facet | Boping Ye Wutong Wu Qian Li Wenping Zhang Zhengbing Lv Yu Ou |
| author_sort | Boping Ye |
| collection | DOAJ |
| description | The Active Peptide from Shark Liver (APSL) was expressed in E. coli BL21 cells. The cDNA encoding APSL protein was obtained from shark regenerated hepatic tissue by RT-PCR, then it was cloned in the pET-28a expression vector. The expressed fusion protein was purified by Ni-IDA affinity chromatography. SDS-PAGE and HPLC analysis showed the purity of the purified fusion protein was more than 98%. The recombinant APSL (rAPSL) was tested for its biological activity both in vitro, by its ability to improve the proliferation of SMMC7721 cells, and in vivo, by its significant protective effects against acute hepatic injury induced by CCl4 and AAP (acetaminophen) in mice. In addition, the rAPSL could decrease the blood glucose concentration of mice with diabetes mellitus induced by alloxan. Paraffin sections of mouse pancreas tissues showed that rAPSL (3 mg/kg) could effectively protect mouse islets from lesions induced by alloxan, which indicated its potential application in theoretical research and industry. |
| first_indexed | 2024-04-14T06:17:43Z |
| format | Article |
| id | doaj.art-5f8ca8054e6f482b9c58cbc7494c02ab |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-14T06:17:43Z |
| publishDate | 2009-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-5f8ca8054e6f482b9c58cbc7494c02ab2022-12-22T02:08:07ZengMDPI AGMarine Drugs1660-33972009-06-017225826710.3390/md7020258Expression, Purification and Bioactivities Analysis of Recombinant Active Peptide from Shark LiverBoping YeWutong WuQian LiWenping ZhangZhengbing LvYu OuThe Active Peptide from Shark Liver (APSL) was expressed in E. coli BL21 cells. The cDNA encoding APSL protein was obtained from shark regenerated hepatic tissue by RT-PCR, then it was cloned in the pET-28a expression vector. The expressed fusion protein was purified by Ni-IDA affinity chromatography. SDS-PAGE and HPLC analysis showed the purity of the purified fusion protein was more than 98%. The recombinant APSL (rAPSL) was tested for its biological activity both in vitro, by its ability to improve the proliferation of SMMC7721 cells, and in vivo, by its significant protective effects against acute hepatic injury induced by CCl4 and AAP (acetaminophen) in mice. In addition, the rAPSL could decrease the blood glucose concentration of mice with diabetes mellitus induced by alloxan. Paraffin sections of mouse pancreas tissues showed that rAPSL (3 mg/kg) could effectively protect mouse islets from lesions induced by alloxan, which indicated its potential application in theoretical research and industry.http://www.mdpi.com/1660-3397/7/2/258/APSLprokaryotic expressionbioactivity analysis |
| spellingShingle | Boping Ye Wutong Wu Qian Li Wenping Zhang Zhengbing Lv Yu Ou Expression, Purification and Bioactivities Analysis of Recombinant Active Peptide from Shark Liver Marine Drugs APSL prokaryotic expression bioactivity analysis |
| title | Expression, Purification and Bioactivities Analysis of Recombinant Active Peptide from Shark Liver |
| title_full | Expression, Purification and Bioactivities Analysis of Recombinant Active Peptide from Shark Liver |
| title_fullStr | Expression, Purification and Bioactivities Analysis of Recombinant Active Peptide from Shark Liver |
| title_full_unstemmed | Expression, Purification and Bioactivities Analysis of Recombinant Active Peptide from Shark Liver |
| title_short | Expression, Purification and Bioactivities Analysis of Recombinant Active Peptide from Shark Liver |
| title_sort | expression purification and bioactivities analysis of recombinant active peptide from shark liver |
| topic | APSL prokaryotic expression bioactivity analysis |
| url | http://www.mdpi.com/1660-3397/7/2/258/ |
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