Some hematological parameters and the prognostic values of CD4, CD8 and total lymphocyte counts and CD4/CD8 cell count ratio in healthy HIV sero-negative, healthy HIV sero-positive and AIDS subjects in Port Harcourt, Nigeria
OBJECTIVE: The present study attempts to determine normal values of CD4, CD8, CD4/CD8 ratio, total WBC and differential counts, hematocrit and total lymphocyte count (TLC) in healthy HIV sero-negative and sero-positive subjects, and to assess the prognostic significance of these parameters in these...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2008-12-01
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| Series: | Turkish Journal of Hematology |
| Subjects: | |
| Online Access: | http://www.tjh.com.tr/jvi.asp?pdir=tjh&plng=eng&un=TJH-29290&look4= |
| _version_ | 1797704729899302912 |
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| author | Blessing Didia Pedro Emem-Chioma Victor Dapper |
| author_facet | Blessing Didia Pedro Emem-Chioma Victor Dapper |
| author_sort | Blessing Didia |
| collection | DOAJ |
| description | OBJECTIVE: The present study attempts to determine normal values of CD4, CD8, CD4/CD8 ratio, total WBC and differential counts, hematocrit and total lymphocyte count (TLC) in healthy HIV sero-negative and sero-positive subjects, and to assess the prognostic significance of these parameters in these subjects as compared to AIDS subjects.METHODS: A total of 300 subjects (147 M, 153 F) aged between 17 and 71 years were recruited into the study. Subjects were separated according to sex and divided into three groups: Group A: healthy HIV sero-negative subjects; Group B: healthy HIV sero-positive newly diagnosed ART-naïve subjects; and Group C: AIDS subjects. CD4 and CD8 counts were determined by flow cytometry; hematocrit was determined using Hawksley micro-capillary tubes; total WBC and differential counts were determined manually with the improved Neubauer counting chamber; and TLC was obtained by multiplying the percentage of lymphocytes by the total WBC count.RESULTS: For male subjects, significant differences were found in CD4 count, CD4/CD8 count ratio, hematocrit, total WBC and TLC, whereas for female subjects, significant differences were found only in CD4 and CD4/CD8 count ratio in the three groups of subjects. In both sexes, however, these parameters were found to be highest in healthy HIV sero-negative subjects and lowest in AIDS subjects, with HIV sero-positive subjects having intermediate values. CONCLUSION: The results confirm previous reports that the CD4 count and CD4/CD8 count ratio are fairly reliable indicators of the progression of HIV infection. In addition, the results also apparently suggest that the prognostic value of CD8 count is limited and that of TLC possibly sex-dependent. The results could be of importance in our environment since previous reports have been relatively scarce. |
| first_indexed | 2024-03-12T05:24:43Z |
| format | Article |
| id | doaj.art-5f8d4c66f3864a7bbde7c708058abead |
| institution | Directory Open Access Journal |
| issn | 1300-7777 1308-5263 |
| language | English |
| last_indexed | 2024-03-12T05:24:43Z |
| publishDate | 2008-12-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Hematology |
| spelling | doaj.art-5f8d4c66f3864a7bbde7c708058abead2023-09-03T07:24:52ZengGalenos Publishing HouseTurkish Journal of Hematology1300-77771308-52632008-12-012504181186Some hematological parameters and the prognostic values of CD4, CD8 and total lymphocyte counts and CD4/CD8 cell count ratio in healthy HIV sero-negative, healthy HIV sero-positive and AIDS subjects in Port Harcourt, NigeriaBlessing DidiaPedro Emem-ChiomaVictor DapperOBJECTIVE: The present study attempts to determine normal values of CD4, CD8, CD4/CD8 ratio, total WBC and differential counts, hematocrit and total lymphocyte count (TLC) in healthy HIV sero-negative and sero-positive subjects, and to assess the prognostic significance of these parameters in these subjects as compared to AIDS subjects.METHODS: A total of 300 subjects (147 M, 153 F) aged between 17 and 71 years were recruited into the study. Subjects were separated according to sex and divided into three groups: Group A: healthy HIV sero-negative subjects; Group B: healthy HIV sero-positive newly diagnosed ART-naïve subjects; and Group C: AIDS subjects. CD4 and CD8 counts were determined by flow cytometry; hematocrit was determined using Hawksley micro-capillary tubes; total WBC and differential counts were determined manually with the improved Neubauer counting chamber; and TLC was obtained by multiplying the percentage of lymphocytes by the total WBC count.RESULTS: For male subjects, significant differences were found in CD4 count, CD4/CD8 count ratio, hematocrit, total WBC and TLC, whereas for female subjects, significant differences were found only in CD4 and CD4/CD8 count ratio in the three groups of subjects. In both sexes, however, these parameters were found to be highest in healthy HIV sero-negative subjects and lowest in AIDS subjects, with HIV sero-positive subjects having intermediate values. CONCLUSION: The results confirm previous reports that the CD4 count and CD4/CD8 count ratio are fairly reliable indicators of the progression of HIV infection. In addition, the results also apparently suggest that the prognostic value of CD8 count is limited and that of TLC possibly sex-dependent. The results could be of importance in our environment since previous reports have been relatively scarce.http://www.tjh.com.tr/jvi.asp?pdir=tjh&plng=eng&un=TJH-29290&look4=CD4 countsCD8 countsCD4/CD8 ratiototal lymphocyte counthuman immunodeficiency virus (HIV)acquired immunodeficiency syndrome (AIDS). |
| spellingShingle | Blessing Didia Pedro Emem-Chioma Victor Dapper Some hematological parameters and the prognostic values of CD4, CD8 and total lymphocyte counts and CD4/CD8 cell count ratio in healthy HIV sero-negative, healthy HIV sero-positive and AIDS subjects in Port Harcourt, Nigeria Turkish Journal of Hematology CD4 counts CD8 counts CD4/CD8 ratio total lymphocyte count human immunodeficiency virus (HIV) acquired immunodeficiency syndrome (AIDS). |
| title | Some hematological parameters and the prognostic values of CD4, CD8 and total lymphocyte counts and CD4/CD8 cell count ratio in healthy HIV sero-negative, healthy HIV sero-positive and AIDS subjects in Port Harcourt, Nigeria |
| title_full | Some hematological parameters and the prognostic values of CD4, CD8 and total lymphocyte counts and CD4/CD8 cell count ratio in healthy HIV sero-negative, healthy HIV sero-positive and AIDS subjects in Port Harcourt, Nigeria |
| title_fullStr | Some hematological parameters and the prognostic values of CD4, CD8 and total lymphocyte counts and CD4/CD8 cell count ratio in healthy HIV sero-negative, healthy HIV sero-positive and AIDS subjects in Port Harcourt, Nigeria |
| title_full_unstemmed | Some hematological parameters and the prognostic values of CD4, CD8 and total lymphocyte counts and CD4/CD8 cell count ratio in healthy HIV sero-negative, healthy HIV sero-positive and AIDS subjects in Port Harcourt, Nigeria |
| title_short | Some hematological parameters and the prognostic values of CD4, CD8 and total lymphocyte counts and CD4/CD8 cell count ratio in healthy HIV sero-negative, healthy HIV sero-positive and AIDS subjects in Port Harcourt, Nigeria |
| title_sort | some hematological parameters and the prognostic values of cd4 cd8 and total lymphocyte counts and cd4 cd8 cell count ratio in healthy hiv sero negative healthy hiv sero positive and aids subjects in port harcourt nigeria |
| topic | CD4 counts CD8 counts CD4/CD8 ratio total lymphocyte count human immunodeficiency virus (HIV) acquired immunodeficiency syndrome (AIDS). |
| url | http://www.tjh.com.tr/jvi.asp?pdir=tjh&plng=eng&un=TJH-29290&look4= |
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