Analysis of Relative Expression of Key Enzyme Genes and Enzyme Activity in Nitrogen Metabolic Pathway of Two Genotypes of Potato (<i>Solanum tuberosum</i> L.) under Different Nitrogen Supply Levels
Nitrogen (N) plays an important role in the growth cycle of the potato, and is an important guarantee of yield and quality. Rational N application is one of the key ways to improve a crop’s high and stable yield and economic and environmental benefits. The N nutrition level of potato regulates the g...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/9/769 |
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| author | Zhijun Han Yue Lu Yanfei Zhao Yaping Wang Zhongcai Han Yuzhu Han Jingying Zhang |
| author_facet | Zhijun Han Yue Lu Yanfei Zhao Yaping Wang Zhongcai Han Yuzhu Han Jingying Zhang |
| author_sort | Zhijun Han |
| collection | DOAJ |
| description | Nitrogen (N) plays an important role in the growth cycle of the potato, and is an important guarantee of yield and quality. Rational N application is one of the key ways to improve a crop’s high and stable yield and economic and environmental benefits. The N nutrition level of potato regulates the gene expression of enzymes related to the N metabolism pathway, which shows the change of the activity of key enzymes in N metabolism, and finally realizes the regulation of N absorption and utilization. In this study, the key enzyme genes and enzyme activity of different genotypes of potato under different N supply treatments were identified, which laid a foundation for further exploration of the functions of each gene in the potato N metabolism pathway and provided theoretical basis for rational N application. The results showed that the relative expression levels of <i>StNRT1.5</i>, <i>StNR</i>, <i>StNiR</i> in leaves, <i>StNRT2.5</i>, <i>StNRT2.4</i>, <i>StGS1-2</i> in roots and <i>StNRT2.7</i>, <i>StGS1-3</i>, <i>StGS2</i>, <i>StGS1-4</i>, <i>StFd-GOGAT</i> in leaves and roots showed that the levels of N-inefficient potato Atlantic (A) were higher than the N-efficient potato Yanshu 4 (Y), while the relative expression levels of <i>StNRT2.5</i>, <i>StGS1-2</i> in leaves, <i>StNRT1.5</i><i>StNR</i>, <i>StNiR</i> in roots and <i>StGDH</i>, <i>StNADH-GOGAT</i> in leaves and roots showed that levels in Yanshu 4 (Y) were higher than in Atlantic (A). At the same time, we especially found that the GDH activity in the leaves of the two genotypes of potato were higher at low N levels. Additionally, the activity of NR and NiR, and the activity of GS and GOGAT were correlated. In addition, the changes of key enzymes in different N metabolism showed a certain continuity with the advancement of growth and development, and some gene expression rules and enzyme activity changes also showed a certain consistency. |
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| spelling | doaj.art-5f902c1de97647fd856d30f122fe80ee2023-11-23T16:33:20ZengMDPI AGHorticulturae2311-75242022-08-018976910.3390/horticulturae8090769Analysis of Relative Expression of Key Enzyme Genes and Enzyme Activity in Nitrogen Metabolic Pathway of Two Genotypes of Potato (<i>Solanum tuberosum</i> L.) under Different Nitrogen Supply LevelsZhijun Han0Yue Lu1Yanfei Zhao2Yaping Wang3Zhongcai Han4Yuzhu Han5Jingying Zhang6College of Horticulture, Jilin Agricultural University, Changchun 130118, ChinaCollege of Horticulture, Jilin Agricultural University, Changchun 130118, ChinaJilin Provincial Research Institute of Vegetables and Flowers, Changchun 130119, ChinaCollege of Horticulture, Jilin Agricultural University, Changchun 130118, ChinaJilin Provincial Research Institute of Vegetables and Flowers, Changchun 130119, ChinaCollege of Horticulture, Jilin Agricultural University, Changchun 130118, ChinaCollege of Horticulture, Jilin Agricultural University, Changchun 130118, ChinaNitrogen (N) plays an important role in the growth cycle of the potato, and is an important guarantee of yield and quality. Rational N application is one of the key ways to improve a crop’s high and stable yield and economic and environmental benefits. The N nutrition level of potato regulates the gene expression of enzymes related to the N metabolism pathway, which shows the change of the activity of key enzymes in N metabolism, and finally realizes the regulation of N absorption and utilization. In this study, the key enzyme genes and enzyme activity of different genotypes of potato under different N supply treatments were identified, which laid a foundation for further exploration of the functions of each gene in the potato N metabolism pathway and provided theoretical basis for rational N application. The results showed that the relative expression levels of <i>StNRT1.5</i>, <i>StNR</i>, <i>StNiR</i> in leaves, <i>StNRT2.5</i>, <i>StNRT2.4</i>, <i>StGS1-2</i> in roots and <i>StNRT2.7</i>, <i>StGS1-3</i>, <i>StGS2</i>, <i>StGS1-4</i>, <i>StFd-GOGAT</i> in leaves and roots showed that the levels of N-inefficient potato Atlantic (A) were higher than the N-efficient potato Yanshu 4 (Y), while the relative expression levels of <i>StNRT2.5</i>, <i>StGS1-2</i> in leaves, <i>StNRT1.5</i><i>StNR</i>, <i>StNiR</i> in roots and <i>StGDH</i>, <i>StNADH-GOGAT</i> in leaves and roots showed that levels in Yanshu 4 (Y) were higher than in Atlantic (A). At the same time, we especially found that the GDH activity in the leaves of the two genotypes of potato were higher at low N levels. Additionally, the activity of NR and NiR, and the activity of GS and GOGAT were correlated. In addition, the changes of key enzymes in different N metabolism showed a certain continuity with the advancement of growth and development, and some gene expression rules and enzyme activity changes also showed a certain consistency.https://www.mdpi.com/2311-7524/8/9/769nitrogen efficiencynitrogen metabolism pathwaykey enzyme genekey enzyme activity |
| spellingShingle | Zhijun Han Yue Lu Yanfei Zhao Yaping Wang Zhongcai Han Yuzhu Han Jingying Zhang Analysis of Relative Expression of Key Enzyme Genes and Enzyme Activity in Nitrogen Metabolic Pathway of Two Genotypes of Potato (<i>Solanum tuberosum</i> L.) under Different Nitrogen Supply Levels Horticulturae nitrogen efficiency nitrogen metabolism pathway key enzyme gene key enzyme activity |
| title | Analysis of Relative Expression of Key Enzyme Genes and Enzyme Activity in Nitrogen Metabolic Pathway of Two Genotypes of Potato (<i>Solanum tuberosum</i> L.) under Different Nitrogen Supply Levels |
| title_full | Analysis of Relative Expression of Key Enzyme Genes and Enzyme Activity in Nitrogen Metabolic Pathway of Two Genotypes of Potato (<i>Solanum tuberosum</i> L.) under Different Nitrogen Supply Levels |
| title_fullStr | Analysis of Relative Expression of Key Enzyme Genes and Enzyme Activity in Nitrogen Metabolic Pathway of Two Genotypes of Potato (<i>Solanum tuberosum</i> L.) under Different Nitrogen Supply Levels |
| title_full_unstemmed | Analysis of Relative Expression of Key Enzyme Genes and Enzyme Activity in Nitrogen Metabolic Pathway of Two Genotypes of Potato (<i>Solanum tuberosum</i> L.) under Different Nitrogen Supply Levels |
| title_short | Analysis of Relative Expression of Key Enzyme Genes and Enzyme Activity in Nitrogen Metabolic Pathway of Two Genotypes of Potato (<i>Solanum tuberosum</i> L.) under Different Nitrogen Supply Levels |
| title_sort | analysis of relative expression of key enzyme genes and enzyme activity in nitrogen metabolic pathway of two genotypes of potato i solanum tuberosum i l under different nitrogen supply levels |
| topic | nitrogen efficiency nitrogen metabolism pathway key enzyme gene key enzyme activity |
| url | https://www.mdpi.com/2311-7524/8/9/769 |
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