Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefinery
Abstract Formic acid (FA) has emerged as a promising one-carbon feedstock for biorefinery. However, developing efficient microbial hosts for economically competitive FA utilization remains a grand challenge. Here, we discover that the bacterium Vibrio natriegens has exceptional FA tolerance and meta...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-43631-2 |
| _version_ | 1797414586960314368 |
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| author | Jinzhong Tian Wangshuying Deng Ziwen Zhang Jiaqi Xu Guiling Yang Guoping Zhao Sheng Yang Weihong Jiang Yang Gu |
| author_facet | Jinzhong Tian Wangshuying Deng Ziwen Zhang Jiaqi Xu Guiling Yang Guoping Zhao Sheng Yang Weihong Jiang Yang Gu |
| author_sort | Jinzhong Tian |
| collection | DOAJ |
| description | Abstract Formic acid (FA) has emerged as a promising one-carbon feedstock for biorefinery. However, developing efficient microbial hosts for economically competitive FA utilization remains a grand challenge. Here, we discover that the bacterium Vibrio natriegens has exceptional FA tolerance and metabolic capacity natively. This bacterium is remodeled by rewiring the serine cycle and the TCA cycle, resulting in a non-native closed loop (S-TCA) which as a powerful metabolic sink, in combination with laboratory evolution, enables rapid emergence of synthetic strains with significantly improved FA-utilizing ability. Further introduction of a foreign indigoidine-forming pathway into the synthetic V. natriegens strain leads to the production of 29.0 g · L−1 indigoidine and consumption of 165.3 g · L−1 formate within 72 h, achieving a formate consumption rate of 2.3 g · L−1 · h−1. This work provides an important microbial chassis as well as design rules to develop industrially viable microorganisms for FA biorefinery. |
| first_indexed | 2024-03-09T05:35:32Z |
| format | Article |
| id | doaj.art-5f9b219f2d07476c81750933c4445a6d |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-09T05:35:32Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-5f9b219f2d07476c81750933c4445a6d2023-12-03T12:28:55ZengNature PortfolioNature Communications2041-17232023-11-0114111210.1038/s41467-023-43631-2Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefineryJinzhong Tian0Wangshuying Deng1Ziwen Zhang2Jiaqi Xu3Guiling Yang4Guoping Zhao5Sheng Yang6Weihong Jiang7Yang Gu8CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of SciencesCAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of SciencesCAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of SciencesZJU-Hangzhou Global Scientific and Technological Innovation CenterXianghu LaboratoryCAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of SciencesCAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of SciencesCAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of SciencesCAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of SciencesAbstract Formic acid (FA) has emerged as a promising one-carbon feedstock for biorefinery. However, developing efficient microbial hosts for economically competitive FA utilization remains a grand challenge. Here, we discover that the bacterium Vibrio natriegens has exceptional FA tolerance and metabolic capacity natively. This bacterium is remodeled by rewiring the serine cycle and the TCA cycle, resulting in a non-native closed loop (S-TCA) which as a powerful metabolic sink, in combination with laboratory evolution, enables rapid emergence of synthetic strains with significantly improved FA-utilizing ability. Further introduction of a foreign indigoidine-forming pathway into the synthetic V. natriegens strain leads to the production of 29.0 g · L−1 indigoidine and consumption of 165.3 g · L−1 formate within 72 h, achieving a formate consumption rate of 2.3 g · L−1 · h−1. This work provides an important microbial chassis as well as design rules to develop industrially viable microorganisms for FA biorefinery.https://doi.org/10.1038/s41467-023-43631-2 |
| spellingShingle | Jinzhong Tian Wangshuying Deng Ziwen Zhang Jiaqi Xu Guiling Yang Guoping Zhao Sheng Yang Weihong Jiang Yang Gu Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefinery Nature Communications |
| title | Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefinery |
| title_full | Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefinery |
| title_fullStr | Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefinery |
| title_full_unstemmed | Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefinery |
| title_short | Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefinery |
| title_sort | discovery and remodeling of vibrio natriegens as a microbial platform for efficient formic acid biorefinery |
| url | https://doi.org/10.1038/s41467-023-43631-2 |
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