Transposable Element Expression and Regulation Profile in Gonads of Interspecific Hybrids of <i>Drosophila</i> <i>arizonae</i> and <i>Drosophila mojavensis wrigleyi</i>
Interspecific hybridization may lead to sterility and/or inviability through differential expression of genes and transposable elements (TEs). In <i>Drosophila</i>, studies have reported massive TE mobilization in hybrids from interspecific crosses of species presenting high divergence t...
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MDPI AG
2021-12-01
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| author | Cecília Artico Banho Daniel Siqueira Oliveira Annabelle Haudry Marie Fablet Cristina Vieira Claudia Marcia Aparecida Carareto |
| author_facet | Cecília Artico Banho Daniel Siqueira Oliveira Annabelle Haudry Marie Fablet Cristina Vieira Claudia Marcia Aparecida Carareto |
| author_sort | Cecília Artico Banho |
| collection | DOAJ |
| description | Interspecific hybridization may lead to sterility and/or inviability through differential expression of genes and transposable elements (TEs). In <i>Drosophila</i>, studies have reported massive TE mobilization in hybrids from interspecific crosses of species presenting high divergence times. However, few studies have examined the consequences of TE mobilization upon hybridization in recently diverged species, such as <i>Drosophila arizonae</i> and <i>D. mojavensis.</i> We have sequenced transcriptomes of <i>D. arizonae</i> and the subspecies <i>D. m. wrigleyi</i> and their reciprocal hybrids, as well as piRNAs, to analyze the impact of genomic stress on TE regulation. Our results revealed that the differential expression in both gonadal tissues of parental species was similar. Globally, ovaries and testes showed few deregulated TEs compared with both parental lines. Analyses of small RNA data showed that in ovaries, the TE upregulation is likely due to divergence of copies inherited from parental genomes and lack of piRNAs mapping to them. Nevertheless, in testes, the divergent expression of genes associated with chromatin state and piRNA pathway potentially indicates that TE differential expression is related to the divergence of regulatory genes that play a role in modulating transcriptional and post-transcriptional mechanisms. |
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| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T04:24:45Z |
| publishDate | 2021-12-01 |
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| spelling | doaj.art-5f9c1bee954d478d8bcc9d8e1886943b2023-11-23T07:39:43ZengMDPI AGCells2073-44092021-12-011012357410.3390/cells10123574Transposable Element Expression and Regulation Profile in Gonads of Interspecific Hybrids of <i>Drosophila</i> <i>arizonae</i> and <i>Drosophila mojavensis wrigleyi</i>Cecília Artico Banho0Daniel Siqueira Oliveira1Annabelle Haudry2Marie Fablet3Cristina Vieira4Claudia Marcia Aparecida Carareto5Institute of Biosciences, Humanities and Exact Sciences, São Paulo State University (Unesp), São José do Rio Preto 15054-000, SP, BrazilInstitute of Biosciences, Humanities and Exact Sciences, São Paulo State University (Unesp), São José do Rio Preto 15054-000, SP, BrazilLaboratoire de Biométrie et Biologie Evolutive, Université de Lyon, Université Lyon 1, CNRS, UMR 5558, F-69622 Villeurbanne, FranceLaboratoire de Biométrie et Biologie Evolutive, Université de Lyon, Université Lyon 1, CNRS, UMR 5558, F-69622 Villeurbanne, FranceLaboratoire de Biométrie et Biologie Evolutive, Université de Lyon, Université Lyon 1, CNRS, UMR 5558, F-69622 Villeurbanne, FranceInstitute of Biosciences, Humanities and Exact Sciences, São Paulo State University (Unesp), São José do Rio Preto 15054-000, SP, BrazilInterspecific hybridization may lead to sterility and/or inviability through differential expression of genes and transposable elements (TEs). In <i>Drosophila</i>, studies have reported massive TE mobilization in hybrids from interspecific crosses of species presenting high divergence times. However, few studies have examined the consequences of TE mobilization upon hybridization in recently diverged species, such as <i>Drosophila arizonae</i> and <i>D. mojavensis.</i> We have sequenced transcriptomes of <i>D. arizonae</i> and the subspecies <i>D. m. wrigleyi</i> and their reciprocal hybrids, as well as piRNAs, to analyze the impact of genomic stress on TE regulation. Our results revealed that the differential expression in both gonadal tissues of parental species was similar. Globally, ovaries and testes showed few deregulated TEs compared with both parental lines. Analyses of small RNA data showed that in ovaries, the TE upregulation is likely due to divergence of copies inherited from parental genomes and lack of piRNAs mapping to them. Nevertheless, in testes, the divergent expression of genes associated with chromatin state and piRNA pathway potentially indicates that TE differential expression is related to the divergence of regulatory genes that play a role in modulating transcriptional and post-transcriptional mechanisms.https://www.mdpi.com/2073-4409/10/12/3574repleta grouphybridstransposable elementsexpression |
| spellingShingle | Cecília Artico Banho Daniel Siqueira Oliveira Annabelle Haudry Marie Fablet Cristina Vieira Claudia Marcia Aparecida Carareto Transposable Element Expression and Regulation Profile in Gonads of Interspecific Hybrids of <i>Drosophila</i> <i>arizonae</i> and <i>Drosophila mojavensis wrigleyi</i> Cells repleta group hybrids transposable elements expression |
| title | Transposable Element Expression and Regulation Profile in Gonads of Interspecific Hybrids of <i>Drosophila</i> <i>arizonae</i> and <i>Drosophila mojavensis wrigleyi</i> |
| title_full | Transposable Element Expression and Regulation Profile in Gonads of Interspecific Hybrids of <i>Drosophila</i> <i>arizonae</i> and <i>Drosophila mojavensis wrigleyi</i> |
| title_fullStr | Transposable Element Expression and Regulation Profile in Gonads of Interspecific Hybrids of <i>Drosophila</i> <i>arizonae</i> and <i>Drosophila mojavensis wrigleyi</i> |
| title_full_unstemmed | Transposable Element Expression and Regulation Profile in Gonads of Interspecific Hybrids of <i>Drosophila</i> <i>arizonae</i> and <i>Drosophila mojavensis wrigleyi</i> |
| title_short | Transposable Element Expression and Regulation Profile in Gonads of Interspecific Hybrids of <i>Drosophila</i> <i>arizonae</i> and <i>Drosophila mojavensis wrigleyi</i> |
| title_sort | transposable element expression and regulation profile in gonads of interspecific hybrids of i drosophila i i arizonae i and i drosophila mojavensis wrigleyi i |
| topic | repleta group hybrids transposable elements expression |
| url | https://www.mdpi.com/2073-4409/10/12/3574 |
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