Protective Effects of Novel <em>Lactobacillaceae</em> Strains Isolated from Chicken Caeca against Necrotic Enteritis Infection: <em>In Vitro</em> and <em>In Vivo</em> Evidences
The present study aimed to show the benefits of novel lactic acid bacteria (LAB) strains isolated from the caeca of healthy chickens. These novel strains, identified as <i>Limosilactobacillus reuteri</i> and <i>Ligilactobacillus salivarius</i>, displayed high levels of lactic...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/1/152 |
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| author | Nuria Vieco-Saiz Yanath Belguesmia Ruth Raspoet Eric Auclair Connor Padgett Christopher Bailey Frédérique Gancel Djamel Drider |
| author_facet | Nuria Vieco-Saiz Yanath Belguesmia Ruth Raspoet Eric Auclair Connor Padgett Christopher Bailey Frédérique Gancel Djamel Drider |
| author_sort | Nuria Vieco-Saiz |
| collection | DOAJ |
| description | The present study aimed to show the benefits of novel lactic acid bacteria (LAB) strains isolated from the caeca of healthy chickens. These novel strains, identified as <i>Limosilactobacillus reuteri</i> and <i>Ligilactobacillus salivarius</i>, displayed high levels of lactic acid production, capability of biofilm formation, high aggregation and adhesion scores, and significant survival rates under conditions mimicking the chicken gastrointestinal tract (GIT). In addition, these novel <i>Lactobacillaceae</i> isolates were neither hemolytic nor cytotoxic. <i>In vivo</i> trials were able to establish their ability to reduce necrotic enteritis. Notably, a significant weight gain was registered, on day 10 of treatment, in the group of chickens fed with a mixture of <i>L.</i> <i>reuteri</i> ICVB416 and <i>L.</i> <i>salivarius</i> ICVB430 strains, as compared with the control group. This group has also shown a reduced number of lesions in the gut compared with other infected chicken groups. This study provides <i>in vitro</i> and <i>in vivo</i> evidence supporting the benefits of these novel <i>Lactobacillaceae</i> isolates for their use in poultry livestock as protective cultures to control the bacterial necrotic enteritis (NE) <i>Clostridium perfringens</i>. |
| first_indexed | 2024-03-10T00:52:51Z |
| format | Article |
| id | doaj.art-5f9d82da9bad4690a2d4d092ac234bd3 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T00:52:51Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-5f9d82da9bad4690a2d4d092ac234bd32023-11-23T14:48:06ZengMDPI AGMicroorganisms2076-26072022-01-0110115210.3390/microorganisms10010152Protective Effects of Novel <em>Lactobacillaceae</em> Strains Isolated from Chicken Caeca against Necrotic Enteritis Infection: <em>In Vitro</em> and <em>In Vivo</em> EvidencesNuria Vieco-Saiz0Yanath Belguesmia1Ruth Raspoet2Eric Auclair3Connor Padgett4Christopher Bailey5Frédérique Gancel6Djamel Drider7UMR Transfrontalière BioEcoAgro1158, Univ. Lille, INRAE, Univ. Liège, UPJV, YNCREA, Univ. Artois, Univ. Littoral Côte d’Opale, ICV—Institut Charles Viollette, F-59000 Lille, FranceUMR Transfrontalière BioEcoAgro1158, Univ. Lille, INRAE, Univ. Liège, UPJV, YNCREA, Univ. Artois, Univ. Littoral Côte d’Opale, ICV—Institut Charles Viollette, F-59000 Lille, FrancePhileo Lesaffre Animal Care, 137 Rue Gabriel Péri, F-59700 Marcq-en-Barœul, FrancePhileo Lesaffre Animal Care, 137 Rue Gabriel Péri, F-59700 Marcq-en-Barœul, FrancePhileo by Lesaffre, 7475 W Main St., Milwaukee, WI 53214, USADepartment of Poultry Science, Texas A&M University, 101 Kleberg Center, 2472 TAMU, College Station, TX 77845, USAUMR Transfrontalière BioEcoAgro1158, Univ. Lille, INRAE, Univ. Liège, UPJV, YNCREA, Univ. Artois, Univ. Littoral Côte d’Opale, ICV—Institut Charles Viollette, F-59000 Lille, FranceUMR Transfrontalière BioEcoAgro1158, Univ. Lille, INRAE, Univ. Liège, UPJV, YNCREA, Univ. Artois, Univ. Littoral Côte d’Opale, ICV—Institut Charles Viollette, F-59000 Lille, FranceThe present study aimed to show the benefits of novel lactic acid bacteria (LAB) strains isolated from the caeca of healthy chickens. These novel strains, identified as <i>Limosilactobacillus reuteri</i> and <i>Ligilactobacillus salivarius</i>, displayed high levels of lactic acid production, capability of biofilm formation, high aggregation and adhesion scores, and significant survival rates under conditions mimicking the chicken gastrointestinal tract (GIT). In addition, these novel <i>Lactobacillaceae</i> isolates were neither hemolytic nor cytotoxic. <i>In vivo</i> trials were able to establish their ability to reduce necrotic enteritis. Notably, a significant weight gain was registered, on day 10 of treatment, in the group of chickens fed with a mixture of <i>L.</i> <i>reuteri</i> ICVB416 and <i>L.</i> <i>salivarius</i> ICVB430 strains, as compared with the control group. This group has also shown a reduced number of lesions in the gut compared with other infected chicken groups. This study provides <i>in vitro</i> and <i>in vivo</i> evidence supporting the benefits of these novel <i>Lactobacillaceae</i> isolates for their use in poultry livestock as protective cultures to control the bacterial necrotic enteritis (NE) <i>Clostridium perfringens</i>.https://www.mdpi.com/2076-2607/10/1/152<i>Limosilactobacillus reuteri</i><i>Ligilactobacillus salivarius</i>probioticscytotoxicitybiofilm<i>Clostridium perfringens</i> |
| spellingShingle | Nuria Vieco-Saiz Yanath Belguesmia Ruth Raspoet Eric Auclair Connor Padgett Christopher Bailey Frédérique Gancel Djamel Drider Protective Effects of Novel <em>Lactobacillaceae</em> Strains Isolated from Chicken Caeca against Necrotic Enteritis Infection: <em>In Vitro</em> and <em>In Vivo</em> Evidences Microorganisms <i>Limosilactobacillus reuteri</i> <i>Ligilactobacillus salivarius</i> probiotics cytotoxicity biofilm <i>Clostridium perfringens</i> |
| title | Protective Effects of Novel <em>Lactobacillaceae</em> Strains Isolated from Chicken Caeca against Necrotic Enteritis Infection: <em>In Vitro</em> and <em>In Vivo</em> Evidences |
| title_full | Protective Effects of Novel <em>Lactobacillaceae</em> Strains Isolated from Chicken Caeca against Necrotic Enteritis Infection: <em>In Vitro</em> and <em>In Vivo</em> Evidences |
| title_fullStr | Protective Effects of Novel <em>Lactobacillaceae</em> Strains Isolated from Chicken Caeca against Necrotic Enteritis Infection: <em>In Vitro</em> and <em>In Vivo</em> Evidences |
| title_full_unstemmed | Protective Effects of Novel <em>Lactobacillaceae</em> Strains Isolated from Chicken Caeca against Necrotic Enteritis Infection: <em>In Vitro</em> and <em>In Vivo</em> Evidences |
| title_short | Protective Effects of Novel <em>Lactobacillaceae</em> Strains Isolated from Chicken Caeca against Necrotic Enteritis Infection: <em>In Vitro</em> and <em>In Vivo</em> Evidences |
| title_sort | protective effects of novel em lactobacillaceae em strains isolated from chicken caeca against necrotic enteritis infection em in vitro em and em in vivo em evidences |
| topic | <i>Limosilactobacillus reuteri</i> <i>Ligilactobacillus salivarius</i> probiotics cytotoxicity biofilm <i>Clostridium perfringens</i> |
| url | https://www.mdpi.com/2076-2607/10/1/152 |
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