Chlorogenic Acid Inhibits <i>Rahnella aquatilis</i> KM25 Growth and Proteolytic Activity in Fish-Based Products
This work verified the antiproliferative and antiproteolytic activities of chlorogenic acid against <i>Rahnella aquatilis</i> KM25, a spoilage organism of raw salmon stored at 4 °C. Chlorogenic acid limited the growth of <i>R. aqatilis</i> KM25 in vitro at a concentration of...
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MDPI AG
2023-05-01
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| Online Access: | https://www.mdpi.com/2076-2607/11/6/1367 |
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| author | Kamila Myszka Natalia Tomaś Wojciech Juzwa Łukasz Wolko |
| author_facet | Kamila Myszka Natalia Tomaś Wojciech Juzwa Łukasz Wolko |
| author_sort | Kamila Myszka |
| collection | DOAJ |
| description | This work verified the antiproliferative and antiproteolytic activities of chlorogenic acid against <i>Rahnella aquatilis</i> KM25, a spoilage organism of raw salmon stored at 4 °C. Chlorogenic acid limited the growth of <i>R. aqatilis</i> KM25 in vitro at a concentration of 2.0 mg/mL. The dead (46%), viable (25%), and injured (20%) cell subpopulations were identified by flow cytometry following treatment of <i>R. aquatilis</i> KM25 with the examined agent. The exposure of <i>R. aquatilis</i> KM25 to chlorogenic acid altered its morphology. Changes in cell dimensions, mostly in length parameters from 0.778 µm to 1.09 µm, were found. The length of untreated cells ranged from 0.958 µm to 1.53 µm. The RT–qPCR experiments revealed changes in the expression of genes responsible for the proliferation and proteolytic activity of cells. Chlorogenic acid caused a significant reduction in the mRNA levels of the <i>ftsZ</i>, <i>ftsA</i>, <i>ftsN</i>, <i>tolB,</i> and <i>M4</i> genes (−2.5, −1.5, −2.0, −1.5, and −1.5, respectively). In situ experiments confirmed the potential of chlorogenic acid to limit bacterial growth. A similar effect was noted in samples treated with benzoic acid, where the growth inhibition of <i>R. aquatilis</i> KM25 was 85–95%. Reduction of microbial <i>R. aquatilis</i> KM25 proliferation significantly limited total volatile base nitrogen (TVB-N) and trimethylamine (TMA-N) formation during storage, extending the shelf life of model products. The TVB-N and TMA-N parameters did not exceed the upper levels of the maximum permissible limit of acceptability. In this work, the TVB-N and TMA-N parameters were 10–25 mg/100 g and 2.5–20.5 mg/100 g, respectively; for samples with benzoic acid-supplemented marinades, the parameters TVB-N and TMA-N were 7.5–25.0 mg/100 g and 2.0–20.0 mg/100 g, respectively. Based on the results of this work, it can be concluded that chlorogenic acid can increase the safety, shelf life, and quality of fishery products. |
| first_indexed | 2024-03-11T02:08:14Z |
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| language | English |
| last_indexed | 2024-03-11T02:08:14Z |
| publishDate | 2023-05-01 |
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| spelling | doaj.art-5fa07f5693ae426fa0c56333145910bf2023-11-18T11:40:56ZengMDPI AGMicroorganisms2076-26072023-05-01116136710.3390/microorganisms11061367Chlorogenic Acid Inhibits <i>Rahnella aquatilis</i> KM25 Growth and Proteolytic Activity in Fish-Based ProductsKamila Myszka0Natalia Tomaś1Wojciech Juzwa2Łukasz Wolko3Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627 Poznan, PolandDepartment of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627 Poznan, PolandDepartment of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627 Poznan, PolandDepartment of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, PolandThis work verified the antiproliferative and antiproteolytic activities of chlorogenic acid against <i>Rahnella aquatilis</i> KM25, a spoilage organism of raw salmon stored at 4 °C. Chlorogenic acid limited the growth of <i>R. aqatilis</i> KM25 in vitro at a concentration of 2.0 mg/mL. The dead (46%), viable (25%), and injured (20%) cell subpopulations were identified by flow cytometry following treatment of <i>R. aquatilis</i> KM25 with the examined agent. The exposure of <i>R. aquatilis</i> KM25 to chlorogenic acid altered its morphology. Changes in cell dimensions, mostly in length parameters from 0.778 µm to 1.09 µm, were found. The length of untreated cells ranged from 0.958 µm to 1.53 µm. The RT–qPCR experiments revealed changes in the expression of genes responsible for the proliferation and proteolytic activity of cells. Chlorogenic acid caused a significant reduction in the mRNA levels of the <i>ftsZ</i>, <i>ftsA</i>, <i>ftsN</i>, <i>tolB,</i> and <i>M4</i> genes (−2.5, −1.5, −2.0, −1.5, and −1.5, respectively). In situ experiments confirmed the potential of chlorogenic acid to limit bacterial growth. A similar effect was noted in samples treated with benzoic acid, where the growth inhibition of <i>R. aquatilis</i> KM25 was 85–95%. Reduction of microbial <i>R. aquatilis</i> KM25 proliferation significantly limited total volatile base nitrogen (TVB-N) and trimethylamine (TMA-N) formation during storage, extending the shelf life of model products. The TVB-N and TMA-N parameters did not exceed the upper levels of the maximum permissible limit of acceptability. In this work, the TVB-N and TMA-N parameters were 10–25 mg/100 g and 2.5–20.5 mg/100 g, respectively; for samples with benzoic acid-supplemented marinades, the parameters TVB-N and TMA-N were 7.5–25.0 mg/100 g and 2.0–20.0 mg/100 g, respectively. Based on the results of this work, it can be concluded that chlorogenic acid can increase the safety, shelf life, and quality of fishery products.https://www.mdpi.com/2076-2607/11/6/1367spoilage of fish-based products<i>Rahnella aquatilis</i>chlorogenic acidproliferation of cellsproteolytic activity |
| spellingShingle | Kamila Myszka Natalia Tomaś Wojciech Juzwa Łukasz Wolko Chlorogenic Acid Inhibits <i>Rahnella aquatilis</i> KM25 Growth and Proteolytic Activity in Fish-Based Products Microorganisms spoilage of fish-based products <i>Rahnella aquatilis</i> chlorogenic acid proliferation of cells proteolytic activity |
| title | Chlorogenic Acid Inhibits <i>Rahnella aquatilis</i> KM25 Growth and Proteolytic Activity in Fish-Based Products |
| title_full | Chlorogenic Acid Inhibits <i>Rahnella aquatilis</i> KM25 Growth and Proteolytic Activity in Fish-Based Products |
| title_fullStr | Chlorogenic Acid Inhibits <i>Rahnella aquatilis</i> KM25 Growth and Proteolytic Activity in Fish-Based Products |
| title_full_unstemmed | Chlorogenic Acid Inhibits <i>Rahnella aquatilis</i> KM25 Growth and Proteolytic Activity in Fish-Based Products |
| title_short | Chlorogenic Acid Inhibits <i>Rahnella aquatilis</i> KM25 Growth and Proteolytic Activity in Fish-Based Products |
| title_sort | chlorogenic acid inhibits i rahnella aquatilis i km25 growth and proteolytic activity in fish based products |
| topic | spoilage of fish-based products <i>Rahnella aquatilis</i> chlorogenic acid proliferation of cells proteolytic activity |
| url | https://www.mdpi.com/2076-2607/11/6/1367 |
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