miR-10a-5p Regulates the Proliferation and Differentiation of Porcine Preadipocytes Targeting the KLF11 Gene
In the swine industry, meat quality, color, and texture are influenced by the excessive differentiation of fat cells. miRNAs have emerged as integral regulators of adipose development. This study delves into the influence of miR-10a-5b on the proliferation and differentiation of pig preadipocytes. O...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/14/2/337 |
| _version_ | 1797344747521572864 |
|---|---|
| author | Wanfeng Zhang Tianzhi Zhao Xinyu Gao Shuangji Ma Tianye Gong Yang Yang Meng Li Guoqing Cao Xiaohong Guo Bugao Li |
| author_facet | Wanfeng Zhang Tianzhi Zhao Xinyu Gao Shuangji Ma Tianye Gong Yang Yang Meng Li Guoqing Cao Xiaohong Guo Bugao Li |
| author_sort | Wanfeng Zhang |
| collection | DOAJ |
| description | In the swine industry, meat quality, color, and texture are influenced by the excessive differentiation of fat cells. miRNAs have emerged as integral regulators of adipose development. This study delves into the influence of miR-10a-5b on the proliferation and differentiation of pig preadipocytes. Our findings reveal that miR-10a-5b is prevalent across various tissues. It hinders preadipocyte proliferation, amplifies the expression of adipogenic genes, promotes lipid accumulation, and, as a result, advances preadipocyte differentiation. We predict that KLF11 is the target gene of miRNA. A dual-fluorescence reporter assay was conducted to validate the binding sites of miR-10a-5b on the 3′UTR of the KLF11 mRNA. Results showed that miR-10a-5b targeted KLF11 3′UTR and reduced the fluorescence activity of the dual-fluorescent reporter vector. Our research also indicates that miR-10a-5b targets and downregulates the expression of both mRNA and the protein levels of KLF11. During the differentiation of the preadipocytes, KLF11 inhibited adipose differentiation and was able to suppress the promotion of adipose differentiation by miR-10a-5b. This underscores miR-10a-5b’s potential as a significant regulator of preadipocyte behavior by modulating KLF11 expression, offering insights into the role of functional miRNAs in fat deposition. |
| first_indexed | 2024-03-08T11:07:13Z |
| format | Article |
| id | doaj.art-5fa12b9b4e984172ac489c9669f8f292 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-08T11:07:13Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-5fa12b9b4e984172ac489c9669f8f2922024-01-26T14:35:16ZengMDPI AGAnimals2076-26152024-01-0114233710.3390/ani14020337miR-10a-5p Regulates the Proliferation and Differentiation of Porcine Preadipocytes Targeting the KLF11 GeneWanfeng Zhang0Tianzhi Zhao1Xinyu Gao2Shuangji Ma3Tianye Gong4Yang Yang5Meng Li6Guoqing Cao7Xiaohong Guo8Bugao Li9College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Jinzhong 030801, ChinaIn the swine industry, meat quality, color, and texture are influenced by the excessive differentiation of fat cells. miRNAs have emerged as integral regulators of adipose development. This study delves into the influence of miR-10a-5b on the proliferation and differentiation of pig preadipocytes. Our findings reveal that miR-10a-5b is prevalent across various tissues. It hinders preadipocyte proliferation, amplifies the expression of adipogenic genes, promotes lipid accumulation, and, as a result, advances preadipocyte differentiation. We predict that KLF11 is the target gene of miRNA. A dual-fluorescence reporter assay was conducted to validate the binding sites of miR-10a-5b on the 3′UTR of the KLF11 mRNA. Results showed that miR-10a-5b targeted KLF11 3′UTR and reduced the fluorescence activity of the dual-fluorescent reporter vector. Our research also indicates that miR-10a-5b targets and downregulates the expression of both mRNA and the protein levels of KLF11. During the differentiation of the preadipocytes, KLF11 inhibited adipose differentiation and was able to suppress the promotion of adipose differentiation by miR-10a-5b. This underscores miR-10a-5b’s potential as a significant regulator of preadipocyte behavior by modulating KLF11 expression, offering insights into the role of functional miRNAs in fat deposition.https://www.mdpi.com/2076-2615/14/2/337miR-10a-5ppigpreadipocyteproliferationdifferentiationKLF11 |
| spellingShingle | Wanfeng Zhang Tianzhi Zhao Xinyu Gao Shuangji Ma Tianye Gong Yang Yang Meng Li Guoqing Cao Xiaohong Guo Bugao Li miR-10a-5p Regulates the Proliferation and Differentiation of Porcine Preadipocytes Targeting the KLF11 Gene Animals miR-10a-5p pig preadipocyte proliferation differentiation KLF11 |
| title | miR-10a-5p Regulates the Proliferation and Differentiation of Porcine Preadipocytes Targeting the KLF11 Gene |
| title_full | miR-10a-5p Regulates the Proliferation and Differentiation of Porcine Preadipocytes Targeting the KLF11 Gene |
| title_fullStr | miR-10a-5p Regulates the Proliferation and Differentiation of Porcine Preadipocytes Targeting the KLF11 Gene |
| title_full_unstemmed | miR-10a-5p Regulates the Proliferation and Differentiation of Porcine Preadipocytes Targeting the KLF11 Gene |
| title_short | miR-10a-5p Regulates the Proliferation and Differentiation of Porcine Preadipocytes Targeting the KLF11 Gene |
| title_sort | mir 10a 5p regulates the proliferation and differentiation of porcine preadipocytes targeting the klf11 gene |
| topic | miR-10a-5p pig preadipocyte proliferation differentiation KLF11 |
| url | https://www.mdpi.com/2076-2615/14/2/337 |
| work_keys_str_mv | AT wanfengzhang mir10a5pregulatestheproliferationanddifferentiationofporcinepreadipocytestargetingtheklf11gene AT tianzhizhao mir10a5pregulatestheproliferationanddifferentiationofporcinepreadipocytestargetingtheklf11gene AT xinyugao mir10a5pregulatestheproliferationanddifferentiationofporcinepreadipocytestargetingtheklf11gene AT shuangjima mir10a5pregulatestheproliferationanddifferentiationofporcinepreadipocytestargetingtheklf11gene AT tianyegong mir10a5pregulatestheproliferationanddifferentiationofporcinepreadipocytestargetingtheklf11gene AT yangyang mir10a5pregulatestheproliferationanddifferentiationofporcinepreadipocytestargetingtheklf11gene AT mengli mir10a5pregulatestheproliferationanddifferentiationofporcinepreadipocytestargetingtheklf11gene AT guoqingcao mir10a5pregulatestheproliferationanddifferentiationofporcinepreadipocytestargetingtheklf11gene AT xiaohongguo mir10a5pregulatestheproliferationanddifferentiationofporcinepreadipocytestargetingtheklf11gene AT bugaoli mir10a5pregulatestheproliferationanddifferentiationofporcinepreadipocytestargetingtheklf11gene |