Phylogenetic analyses of Salmonella detected along the broiler production chain in Trinidad and Tobago
ABSTRACT: This study was conducted to determine the phylogenies of Salmonella strains isolated from cross-sectional studies conducted at hatcheries, broiler farms, processing plants, and retail outlets (broiler production chain) in Trinidad and Tobago over 4 yr (2016–2019). Whole-genome sequencing (...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122006162 |
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| author | Anisa S. Khan Rian E. Pierneef Narjol Gonzalez-Escalona Meghan Maguire Karla Georges Woubit Abebe Abiodun A. Adesiyun |
| author_facet | Anisa S. Khan Rian E. Pierneef Narjol Gonzalez-Escalona Meghan Maguire Karla Georges Woubit Abebe Abiodun A. Adesiyun |
| author_sort | Anisa S. Khan |
| collection | DOAJ |
| description | ABSTRACT: This study was conducted to determine the phylogenies of Salmonella strains isolated from cross-sectional studies conducted at hatcheries, broiler farms, processing plants, and retail outlets (broiler production chain) in Trinidad and Tobago over 4 yr (2016–2019). Whole-genome sequencing (WGS) was used to characterize Salmonella isolates. Core genome phylogenies of 8 serovars of public health significance were analyzed for similarities in origin and relatedness. In addition, Salmonella strains isolated from human salmonellosis cases in Trinidad were analyzed for their relatedness to the isolates detected along the broiler production chain. The common source of these isolates of diverse serovars within farms, within processing plants, between processing plants and retail outlets, and among farm-processing plant-retail outlet continuum was well-supported (bootstrap value >70%) by the core genome phylogenies for the respective serovars. Also, genome analyses revealed clustering of Salmonella serovars of regional (intra-Caribbean) and international (extra-Caribbean) origin. Similarly, strains of S. Enteritidis and S. Infantis isolated from human clinical salmonellosis in 2019 from Trinidad and Tobago clustered with our processing plant isolates recovered in 2018. This study is the first phylogenetic analysis of Salmonella isolates using WGS from the broiler industry in the Caribbean region. The use of WGS confirmed the genetic relatedness and transmission of Salmonella serovars contaminating chickens in broiler processing, and retailing in the country, with zoonotic and food safety implications for humans. |
| first_indexed | 2024-04-10T19:37:38Z |
| format | Article |
| id | doaj.art-5fa1f65141bb4c90acaaabe3e2108abe |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-10T19:37:38Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-5fa1f65141bb4c90acaaabe3e2108abe2023-01-30T04:11:14ZengElsevierPoultry Science0032-57912023-02-011022102322Phylogenetic analyses of Salmonella detected along the broiler production chain in Trinidad and TobagoAnisa S. Khan0Rian E. Pierneef1Narjol Gonzalez-Escalona2Meghan Maguire3Karla Georges4Woubit Abebe5Abiodun A. Adesiyun6School of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, St. Augustine, Trinidad and TobagoAgricultural Research Council-Biotechnology Platform, Pretoria 0110, South AfricaDivision of Microbiology, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD, USADivision of Microbiology, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD, USASchool of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, St. Augustine, Trinidad and TobagoDepartment of Pathobiology, Center for Food Animal Health, Food Safety and Food Defense, Tuskegee University, College of Veterinary Medicine, Tuskegee, AL 36088, USASchool of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, St. Augustine, Trinidad and Tobago; Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Pretoria 0110, South Africa; Corresponding author:ABSTRACT: This study was conducted to determine the phylogenies of Salmonella strains isolated from cross-sectional studies conducted at hatcheries, broiler farms, processing plants, and retail outlets (broiler production chain) in Trinidad and Tobago over 4 yr (2016–2019). Whole-genome sequencing (WGS) was used to characterize Salmonella isolates. Core genome phylogenies of 8 serovars of public health significance were analyzed for similarities in origin and relatedness. In addition, Salmonella strains isolated from human salmonellosis cases in Trinidad were analyzed for their relatedness to the isolates detected along the broiler production chain. The common source of these isolates of diverse serovars within farms, within processing plants, between processing plants and retail outlets, and among farm-processing plant-retail outlet continuum was well-supported (bootstrap value >70%) by the core genome phylogenies for the respective serovars. Also, genome analyses revealed clustering of Salmonella serovars of regional (intra-Caribbean) and international (extra-Caribbean) origin. Similarly, strains of S. Enteritidis and S. Infantis isolated from human clinical salmonellosis in 2019 from Trinidad and Tobago clustered with our processing plant isolates recovered in 2018. This study is the first phylogenetic analysis of Salmonella isolates using WGS from the broiler industry in the Caribbean region. The use of WGS confirmed the genetic relatedness and transmission of Salmonella serovars contaminating chickens in broiler processing, and retailing in the country, with zoonotic and food safety implications for humans.http://www.sciencedirect.com/science/article/pii/S0032579122006162Salmonellabroiler production chainWGSphylogenyTrinidad and Tobago |
| spellingShingle | Anisa S. Khan Rian E. Pierneef Narjol Gonzalez-Escalona Meghan Maguire Karla Georges Woubit Abebe Abiodun A. Adesiyun Phylogenetic analyses of Salmonella detected along the broiler production chain in Trinidad and Tobago Poultry Science Salmonella broiler production chain WGS phylogeny Trinidad and Tobago |
| title | Phylogenetic analyses of Salmonella detected along the broiler production chain in Trinidad and Tobago |
| title_full | Phylogenetic analyses of Salmonella detected along the broiler production chain in Trinidad and Tobago |
| title_fullStr | Phylogenetic analyses of Salmonella detected along the broiler production chain in Trinidad and Tobago |
| title_full_unstemmed | Phylogenetic analyses of Salmonella detected along the broiler production chain in Trinidad and Tobago |
| title_short | Phylogenetic analyses of Salmonella detected along the broiler production chain in Trinidad and Tobago |
| title_sort | phylogenetic analyses of salmonella detected along the broiler production chain in trinidad and tobago |
| topic | Salmonella broiler production chain WGS phylogeny Trinidad and Tobago |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122006162 |
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