Immunization of MERS-CoV-2-infected mice with a sublethal dose of MERS-CoV or VRP-MERS-S
Summary: Here, we detail the immunization of mice with a sublethal dose of MERS-CoV or two doses of replication-incompetent alphavirus replicon particles expressing MERS-CoV spike protein. We then describe steps to determine the outcome of immunization by challenging immunized mice with a lethal dos...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723001296 |
| _version_ | 1797869687707533312 |
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| author | Jian Zheng Rudra Channappanavar Stanley Perlman |
| author_facet | Jian Zheng Rudra Channappanavar Stanley Perlman |
| author_sort | Jian Zheng |
| collection | DOAJ |
| description | Summary: Here, we detail the immunization of mice with a sublethal dose of MERS-CoV or two doses of replication-incompetent alphavirus replicon particles expressing MERS-CoV spike protein. We then describe steps to determine the outcome of immunization by challenging immunized mice with a lethal dose of MERS-CoV, as well as by detecting virus-specific neutralizing antibody and virus-specific T cell response via neutralization assay and flow cytometry, respectively. This protocol can be used to evaluate other CoV infections or vaccine-induced immune responses.For complete details on the use and execution of this protocol, please refer to Zheng et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-10T00:15:26Z |
| format | Article |
| id | doaj.art-5fa5c101c43f46f0909520c29f2616c1 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-10T00:15:26Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-5fa5c101c43f46f0909520c29f2616c12023-03-16T05:06:16ZengElsevierSTAR Protocols2666-16672023-06-0142102171Immunization of MERS-CoV-2-infected mice with a sublethal dose of MERS-CoV or VRP-MERS-SJian Zheng0Rudra Channappanavar1Stanley Perlman2Department of Microbiology and Immunology, School of Medicine, University of Louisville, Louisville, KY 40202, USA; Center for Predictive Medicine, School of Medicine, University of Louisville, Louisville, KY 40202, USA; Departments of Microbiology and Immunology, University of Iowa, Iowa City, IA 52242, USADepartment of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK, USADepartments of Microbiology and Immunology, University of Iowa, Iowa City, IA 52242, USA; Departments of Pediatrics, University of Iowa, Iowa City, IA 52242, USA; Corresponding authorSummary: Here, we detail the immunization of mice with a sublethal dose of MERS-CoV or two doses of replication-incompetent alphavirus replicon particles expressing MERS-CoV spike protein. We then describe steps to determine the outcome of immunization by challenging immunized mice with a lethal dose of MERS-CoV, as well as by detecting virus-specific neutralizing antibody and virus-specific T cell response via neutralization assay and flow cytometry, respectively. This protocol can be used to evaluate other CoV infections or vaccine-induced immune responses.For complete details on the use and execution of this protocol, please refer to Zheng et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723001296Cell BiologyFlow Cytometry/Mass CytometryImmunologyMicrobiologyModel Organisms |
| spellingShingle | Jian Zheng Rudra Channappanavar Stanley Perlman Immunization of MERS-CoV-2-infected mice with a sublethal dose of MERS-CoV or VRP-MERS-S STAR Protocols Cell Biology Flow Cytometry/Mass Cytometry Immunology Microbiology Model Organisms |
| title | Immunization of MERS-CoV-2-infected mice with a sublethal dose of MERS-CoV or VRP-MERS-S |
| title_full | Immunization of MERS-CoV-2-infected mice with a sublethal dose of MERS-CoV or VRP-MERS-S |
| title_fullStr | Immunization of MERS-CoV-2-infected mice with a sublethal dose of MERS-CoV or VRP-MERS-S |
| title_full_unstemmed | Immunization of MERS-CoV-2-infected mice with a sublethal dose of MERS-CoV or VRP-MERS-S |
| title_short | Immunization of MERS-CoV-2-infected mice with a sublethal dose of MERS-CoV or VRP-MERS-S |
| title_sort | immunization of mers cov 2 infected mice with a sublethal dose of mers cov or vrp mers s |
| topic | Cell Biology Flow Cytometry/Mass Cytometry Immunology Microbiology Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723001296 |
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