Immunization of MERS-CoV-2-infected mice with a sublethal dose of MERS-CoV or VRP-MERS-S

Immunization of MERS-CoV-2-infected mice with a sublethal dose of MERS-CoV or VRP-MERS-S

Summary: Here, we detail the immunization of mice with a sublethal dose of MERS-CoV or two doses of replication-incompetent alphavirus replicon particles expressing MERS-CoV spike protein. We then describe steps to determine the outcome of immunization by challenging immunized mice with a lethal dos...

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Bibliographic Details
Main Authors: Jian Zheng, Rudra Channappanavar, Stanley Perlman
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Flow Cytometry/Mass Cytometry
Immunology
Microbiology
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723001296

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