Transcriptome Profile Analyses of Head Kidney in Roach (<i>Rutilus rutilus</i>), Common Bream (<i>Abramis brama</i>) and Their Hybrids: Does Infection by Monogenean Parasites in Freshwater Fish Reveal Differences in Fish Vigour among Parental Species and Their Hybrids?
Hybrid generations usually face either a heterosis advantage or a breakdown, that can be expressed by the level of parasite infection in hybrid hosts. Hybrids are less infected by parasites than parental species (especially F1 generations) or more infected than parental species (especially post-F1 g...
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MDPI AG
2023-09-01
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| Online Access: | https://www.mdpi.com/2079-7737/12/9/1199 |
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| author | Andrea Šimková Kristína Civáňová Křížová Kristýna Voříšková Lukáš Vetešník Vojtěch Bystrý Martin Demko |
| author_facet | Andrea Šimková Kristína Civáňová Křížová Kristýna Voříšková Lukáš Vetešník Vojtěch Bystrý Martin Demko |
| author_sort | Andrea Šimková |
| collection | DOAJ |
| description | Hybrid generations usually face either a heterosis advantage or a breakdown, that can be expressed by the level of parasite infection in hybrid hosts. Hybrids are less infected by parasites than parental species (especially F1 generations) or more infected than parental species (especially post-F1 generations). We performed the experiment with blood-feeding gill parasite <i>Paradiplozoon homoion</i> (Monogenea) infecting leuciscid species, <i>Abramis brama</i> and <i>Rutilus rutilus</i>, their F1 generation and two backcross generations. Backcross generations tended to be more parasitized than parental lines and the F1 generation. The number of differentially expressed genes (DEGs) was lower in F1 hybrids and higher in backcross hybrids when compared to each of the parental lines. The main groups of DEGs were shared among lines; however, <i>A. brama</i> and <i>R. rutilus</i> differed in some of the top gene ontology (GO) terms. DEG analyses revealed the role of heme binding and erythrocyte differentiation after infection by blood-feeding <i>P. homoion</i>. Two backcross generations shared some of the top GO terms, representing mostly downregulated genes associated with <i>P. homoion</i> infection. KEGG analysis revealed the importance of disease-associated pathways; the majority of them were shared by two backcross generations. Our study revealed the most pronounced DEGs associated with blood-feeding monogeneans in backcross hybrids, potentially (but not exclusively) explainable by hybrid breakdown. The lower DEGs reported in F1 hybrids being less parasitized than backcross hybrids is in line with the hybrid advantage. |
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| issn | 2079-7737 |
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| last_indexed | 2024-03-10T23:01:22Z |
| publishDate | 2023-09-01 |
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| series | Biology |
| spelling | doaj.art-5fa7e3af04b14a97a72434676775c8302023-11-19T09:38:25ZengMDPI AGBiology2079-77372023-09-01129119910.3390/biology12091199Transcriptome Profile Analyses of Head Kidney in Roach (<i>Rutilus rutilus</i>), Common Bream (<i>Abramis brama</i>) and Their Hybrids: Does Infection by Monogenean Parasites in Freshwater Fish Reveal Differences in Fish Vigour among Parental Species and Their Hybrids?Andrea Šimková0Kristína Civáňová Křížová1Kristýna Voříšková2Lukáš Vetešník3Vojtěch Bystrý4Martin Demko5Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech RepublicDepartment of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech RepublicDepartment of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech RepublicDepartment of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech RepublicCentral European Institute of Technology, Masaryk University, 625 00 Brno, Czech RepublicDepartment of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech RepublicHybrid generations usually face either a heterosis advantage or a breakdown, that can be expressed by the level of parasite infection in hybrid hosts. Hybrids are less infected by parasites than parental species (especially F1 generations) or more infected than parental species (especially post-F1 generations). We performed the experiment with blood-feeding gill parasite <i>Paradiplozoon homoion</i> (Monogenea) infecting leuciscid species, <i>Abramis brama</i> and <i>Rutilus rutilus</i>, their F1 generation and two backcross generations. Backcross generations tended to be more parasitized than parental lines and the F1 generation. The number of differentially expressed genes (DEGs) was lower in F1 hybrids and higher in backcross hybrids when compared to each of the parental lines. The main groups of DEGs were shared among lines; however, <i>A. brama</i> and <i>R. rutilus</i> differed in some of the top gene ontology (GO) terms. DEG analyses revealed the role of heme binding and erythrocyte differentiation after infection by blood-feeding <i>P. homoion</i>. Two backcross generations shared some of the top GO terms, representing mostly downregulated genes associated with <i>P. homoion</i> infection. KEGG analysis revealed the importance of disease-associated pathways; the majority of them were shared by two backcross generations. Our study revealed the most pronounced DEGs associated with blood-feeding monogeneans in backcross hybrids, potentially (but not exclusively) explainable by hybrid breakdown. The lower DEGs reported in F1 hybrids being less parasitized than backcross hybrids is in line with the hybrid advantage.https://www.mdpi.com/2079-7737/12/9/1199Monogenea<i>Paradiplozoon homoion</i>freshwater fishhybridizationRNA seqdifferential gene expression |
| spellingShingle | Andrea Šimková Kristína Civáňová Křížová Kristýna Voříšková Lukáš Vetešník Vojtěch Bystrý Martin Demko Transcriptome Profile Analyses of Head Kidney in Roach (<i>Rutilus rutilus</i>), Common Bream (<i>Abramis brama</i>) and Their Hybrids: Does Infection by Monogenean Parasites in Freshwater Fish Reveal Differences in Fish Vigour among Parental Species and Their Hybrids? Biology Monogenea <i>Paradiplozoon homoion</i> freshwater fish hybridization RNA seq differential gene expression |
| title | Transcriptome Profile Analyses of Head Kidney in Roach (<i>Rutilus rutilus</i>), Common Bream (<i>Abramis brama</i>) and Their Hybrids: Does Infection by Monogenean Parasites in Freshwater Fish Reveal Differences in Fish Vigour among Parental Species and Their Hybrids? |
| title_full | Transcriptome Profile Analyses of Head Kidney in Roach (<i>Rutilus rutilus</i>), Common Bream (<i>Abramis brama</i>) and Their Hybrids: Does Infection by Monogenean Parasites in Freshwater Fish Reveal Differences in Fish Vigour among Parental Species and Their Hybrids? |
| title_fullStr | Transcriptome Profile Analyses of Head Kidney in Roach (<i>Rutilus rutilus</i>), Common Bream (<i>Abramis brama</i>) and Their Hybrids: Does Infection by Monogenean Parasites in Freshwater Fish Reveal Differences in Fish Vigour among Parental Species and Their Hybrids? |
| title_full_unstemmed | Transcriptome Profile Analyses of Head Kidney in Roach (<i>Rutilus rutilus</i>), Common Bream (<i>Abramis brama</i>) and Their Hybrids: Does Infection by Monogenean Parasites in Freshwater Fish Reveal Differences in Fish Vigour among Parental Species and Their Hybrids? |
| title_short | Transcriptome Profile Analyses of Head Kidney in Roach (<i>Rutilus rutilus</i>), Common Bream (<i>Abramis brama</i>) and Their Hybrids: Does Infection by Monogenean Parasites in Freshwater Fish Reveal Differences in Fish Vigour among Parental Species and Their Hybrids? |
| title_sort | transcriptome profile analyses of head kidney in roach i rutilus rutilus i common bream i abramis brama i and their hybrids does infection by monogenean parasites in freshwater fish reveal differences in fish vigour among parental species and their hybrids |
| topic | Monogenea <i>Paradiplozoon homoion</i> freshwater fish hybridization RNA seq differential gene expression |
| url | https://www.mdpi.com/2079-7737/12/9/1199 |
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