Characterization of genes encoding small heat shock proteins from Bemisia tabaci and expression under thermal stress
Small heat shock proteins (sHSPs) are probably the most diverse in structure and function among the various super-families of stress proteins, and they play essential roles in various biological processes. The sweet potato whitefly, Bemisia tabaci (Gennadius), feeds in the phloem, transmits several...
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PeerJ Inc.
2019-06-01
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| author | Jing Bai Xiao-Na Liu Ming-Xing Lu Yu-Zhou Du |
| author_facet | Jing Bai Xiao-Na Liu Ming-Xing Lu Yu-Zhou Du |
| author_sort | Jing Bai |
| collection | DOAJ |
| description | Small heat shock proteins (sHSPs) are probably the most diverse in structure and function among the various super-families of stress proteins, and they play essential roles in various biological processes. The sweet potato whitefly, Bemisia tabaci (Gennadius), feeds in the phloem, transmits several plant viruses, and is an important pest on cotton, vegetables and ornamentals. In this research, we isolated and characterized three α-crystallin/sHSP family genes (Bthsp19.5, Bthsp19.2, and Bthsp21.3) from Bemisia tabaci. The three cDNAs encoded proteins of 171, 169, and 189 amino acids with calculated molecular weights of 19.5, 19.2, and 21.3 kDa and isoelectric points of 6.1, 6.2, and 6.0, respectively. The deduced amino acid sequences of the three genes showed strong similarity to sHSPs identified in Hemiptera and Thysanoptera insects species. All three sHSPs genes from Bemisia tabaci lacked introns. Quantitative real-time PCR analyses revealed that the three BtsHSPs genes were significantly up-regulated in Bemisia tabaci adults and pupae during high temperature stress (39, 41, 43, and 45 °C) but not in response to cold temperature stress (−6, −8, −10, and −12 °C). The expression levels of Bthsp19.2 and Bthsp21.3 in pupae was higher than adults in response to heat stress, while the expression level of Bthsp19.5 in adults was higher than pupae. In conclusion, this research results show that the sHSP genes of Bemisia tabaci had shown differential expression changes under thermal stress. |
| first_indexed | 2024-03-09T08:01:38Z |
| format | Article |
| id | doaj.art-5fab8d07ffde4409aca21a15e7355be4 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:01:38Z |
| publishDate | 2019-06-01 |
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| spelling | doaj.art-5fab8d07ffde4409aca21a15e7355be42023-12-03T00:42:13ZengPeerJ Inc.PeerJ2167-83592019-06-017e699210.7717/peerj.6992Characterization of genes encoding small heat shock proteins from Bemisia tabaci and expression under thermal stressJing Bai0Xiao-Na Liu1Ming-Xing Lu2Yu-Zhou Du3College of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou, ChinaCollege of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou, ChinaCollege of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou, ChinaCollege of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou, ChinaSmall heat shock proteins (sHSPs) are probably the most diverse in structure and function among the various super-families of stress proteins, and they play essential roles in various biological processes. The sweet potato whitefly, Bemisia tabaci (Gennadius), feeds in the phloem, transmits several plant viruses, and is an important pest on cotton, vegetables and ornamentals. In this research, we isolated and characterized three α-crystallin/sHSP family genes (Bthsp19.5, Bthsp19.2, and Bthsp21.3) from Bemisia tabaci. The three cDNAs encoded proteins of 171, 169, and 189 amino acids with calculated molecular weights of 19.5, 19.2, and 21.3 kDa and isoelectric points of 6.1, 6.2, and 6.0, respectively. The deduced amino acid sequences of the three genes showed strong similarity to sHSPs identified in Hemiptera and Thysanoptera insects species. All three sHSPs genes from Bemisia tabaci lacked introns. Quantitative real-time PCR analyses revealed that the three BtsHSPs genes were significantly up-regulated in Bemisia tabaci adults and pupae during high temperature stress (39, 41, 43, and 45 °C) but not in response to cold temperature stress (−6, −8, −10, and −12 °C). The expression levels of Bthsp19.2 and Bthsp21.3 in pupae was higher than adults in response to heat stress, while the expression level of Bthsp19.5 in adults was higher than pupae. In conclusion, this research results show that the sHSP genes of Bemisia tabaci had shown differential expression changes under thermal stress.https://peerj.com/articles/6992.pdfsHSPsBemisia tabaciDevelopmental stageExpression patternThermal stress |
| spellingShingle | Jing Bai Xiao-Na Liu Ming-Xing Lu Yu-Zhou Du Characterization of genes encoding small heat shock proteins from Bemisia tabaci and expression under thermal stress PeerJ sHSPs Bemisia tabaci Developmental stage Expression pattern Thermal stress |
| title | Characterization of genes encoding small heat shock proteins from Bemisia tabaci and expression under thermal stress |
| title_full | Characterization of genes encoding small heat shock proteins from Bemisia tabaci and expression under thermal stress |
| title_fullStr | Characterization of genes encoding small heat shock proteins from Bemisia tabaci and expression under thermal stress |
| title_full_unstemmed | Characterization of genes encoding small heat shock proteins from Bemisia tabaci and expression under thermal stress |
| title_short | Characterization of genes encoding small heat shock proteins from Bemisia tabaci and expression under thermal stress |
| title_sort | characterization of genes encoding small heat shock proteins from bemisia tabaci and expression under thermal stress |
| topic | sHSPs Bemisia tabaci Developmental stage Expression pattern Thermal stress |
| url | https://peerj.com/articles/6992.pdf |
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