High Productivity of Eicosapentaenoic Acid and Fucoxanthin by a Marine Diatom Chaetoceros gracilis in a Semi-Continuous Culture
Significantly high eicosapentaenoic acid (EPA) and fucoxanthin contents with high production rate were achieved in semi continuous culture of marine diatom. Effects of dilution rate on the production of biomass and high value biocompounds such as EPA and fucoxanthin were evaluated in semi-continuous...
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Frontiers Media S.A.
2020-12-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2020.602721/full |
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| author | Saki Tachihana Norio Nagao Tomoyo Katayama Minamo Hirahara Fatimah Md. Yusoff Sanjoy Banerjee Mohamed Shariff Norio Kurosawa Tatsuki Toda Ken Furuya Ken Furuya |
| author_facet | Saki Tachihana Norio Nagao Tomoyo Katayama Minamo Hirahara Fatimah Md. Yusoff Sanjoy Banerjee Mohamed Shariff Norio Kurosawa Tatsuki Toda Ken Furuya Ken Furuya |
| author_sort | Saki Tachihana |
| collection | DOAJ |
| description | Significantly high eicosapentaenoic acid (EPA) and fucoxanthin contents with high production rate were achieved in semi continuous culture of marine diatom. Effects of dilution rate on the production of biomass and high value biocompounds such as EPA and fucoxanthin were evaluated in semi-continuous cultures of Chaetoceros gracilis under high light condition. Cellular dry weight increased at lower dilution rate and higher light intensity conditions, and cell size strongly affected EPA and fucoxanthin contents. The smaller microalgae cells showed significantly higher (p < 0.05) value of 17.1 mg g-dw–1 fucoxanthin and 41.5% EPA content per total fatty acid compared to those observed in the larger cells. Chaetoceros gracilis can accumulate relatively higher EPA and fucoxanthin than those reported previously. In addition, maintenance of small cell size by supplying sufficient nutrients and light energy can be the key for the increase production of valuable biocompounds in C. gracilis. |
| first_indexed | 2024-12-17T20:37:56Z |
| format | Article |
| id | doaj.art-5faeff9a83a7430b81d8158b2ab146cb |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-17T20:37:56Z |
| publishDate | 2020-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-5faeff9a83a7430b81d8158b2ab146cb2022-12-21T21:33:23ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-12-01810.3389/fbioe.2020.602721602721High Productivity of Eicosapentaenoic Acid and Fucoxanthin by a Marine Diatom Chaetoceros gracilis in a Semi-Continuous CultureSaki Tachihana0Norio Nagao1Tomoyo Katayama2Minamo Hirahara3Fatimah Md. Yusoff4Sanjoy Banerjee5Mohamed Shariff6Norio Kurosawa7Tatsuki Toda8Ken Furuya9Ken Furuya10Department of Science and Engineering for Sustainable Innovation, Faculty of Science and Engineering, Soka University, Tokyo, JapanLaboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, Seri Kembangan, MalaysiaDepartment of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, JapanDepartment of Science and Engineering for Sustainable Innovation, Faculty of Science and Engineering, Soka University, Tokyo, JapanDepartment of Aquaculture, Universiti Putra Malaysia, Seri Kembangan, MalaysiaLaboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, Seri Kembangan, MalaysiaFaculty of Veterinary Medicine, Universiti Putra Malaysia, Seri Kembangan, MalaysiaDepartment of Science and Engineering for Sustainable Innovation, Faculty of Science and Engineering, Soka University, Tokyo, JapanDepartment of Science and Engineering for Sustainable Innovation, Faculty of Science and Engineering, Soka University, Tokyo, JapanDepartment of Science and Engineering for Sustainable Innovation, Faculty of Science and Engineering, Soka University, Tokyo, JapanDepartment of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, JapanSignificantly high eicosapentaenoic acid (EPA) and fucoxanthin contents with high production rate were achieved in semi continuous culture of marine diatom. Effects of dilution rate on the production of biomass and high value biocompounds such as EPA and fucoxanthin were evaluated in semi-continuous cultures of Chaetoceros gracilis under high light condition. Cellular dry weight increased at lower dilution rate and higher light intensity conditions, and cell size strongly affected EPA and fucoxanthin contents. The smaller microalgae cells showed significantly higher (p < 0.05) value of 17.1 mg g-dw–1 fucoxanthin and 41.5% EPA content per total fatty acid compared to those observed in the larger cells. Chaetoceros gracilis can accumulate relatively higher EPA and fucoxanthin than those reported previously. In addition, maintenance of small cell size by supplying sufficient nutrients and light energy can be the key for the increase production of valuable biocompounds in C. gracilis.https://www.frontiersin.org/articles/10.3389/fbioe.2020.602721/fullmicroalgae productioneicosapentaenoic acidfucoxanthinChaetoceros gracilisdilution rate |
| spellingShingle | Saki Tachihana Norio Nagao Tomoyo Katayama Minamo Hirahara Fatimah Md. Yusoff Sanjoy Banerjee Mohamed Shariff Norio Kurosawa Tatsuki Toda Ken Furuya Ken Furuya High Productivity of Eicosapentaenoic Acid and Fucoxanthin by a Marine Diatom Chaetoceros gracilis in a Semi-Continuous Culture Frontiers in Bioengineering and Biotechnology microalgae production eicosapentaenoic acid fucoxanthin Chaetoceros gracilis dilution rate |
| title | High Productivity of Eicosapentaenoic Acid and Fucoxanthin by a Marine Diatom Chaetoceros gracilis in a Semi-Continuous Culture |
| title_full | High Productivity of Eicosapentaenoic Acid and Fucoxanthin by a Marine Diatom Chaetoceros gracilis in a Semi-Continuous Culture |
| title_fullStr | High Productivity of Eicosapentaenoic Acid and Fucoxanthin by a Marine Diatom Chaetoceros gracilis in a Semi-Continuous Culture |
| title_full_unstemmed | High Productivity of Eicosapentaenoic Acid and Fucoxanthin by a Marine Diatom Chaetoceros gracilis in a Semi-Continuous Culture |
| title_short | High Productivity of Eicosapentaenoic Acid and Fucoxanthin by a Marine Diatom Chaetoceros gracilis in a Semi-Continuous Culture |
| title_sort | high productivity of eicosapentaenoic acid and fucoxanthin by a marine diatom chaetoceros gracilis in a semi continuous culture |
| topic | microalgae production eicosapentaenoic acid fucoxanthin Chaetoceros gracilis dilution rate |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2020.602721/full |
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