Effect of Ammonia Pretreatment on Enzymatic Sugar Production from Corn Stover
To make comprehensive use of maize stover, cellulase degradation of maize stover was accelerated. In this paper, maize straw was used as raw material, and reducing sugar yield was the main indicator. The reducing sugar yield was determined by the DNS method after enzymatic digestion of maize straw b...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-06-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022090155 |
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| author | Jianxun LI Xin LI Yumeng WANG Wenjuan ZHANG Jiping WEI Wenjun SONG |
| author_facet | Jianxun LI Xin LI Yumeng WANG Wenjuan ZHANG Jiping WEI Wenjun SONG |
| author_sort | Jianxun LI |
| collection | DOAJ |
| description | To make comprehensive use of maize stover, cellulase degradation of maize stover was accelerated. In this paper, maize straw was used as raw material, and reducing sugar yield was the main indicator. The reducing sugar yield was determined by the DNS method after enzymatic digestion of maize straw by ammonification pretreatment, and the effects of ammonification agent type, concentration, solid content and ammonification time on the enzymatic sugar production from maize straw were investigated. The results showed that the highest reducing sugar yield of 314.18 mg/mL was achieved under this condition with ammonium carbonate as the ammoniating agent, ammoniating agent concentration of 20%, solid content of 50% and ammoniating time of 11 d, which was 51.80% higher compared to direct enzymatic digestion of the straw. In addition, it was found by SEM that the structure of lignin and cellulose changed after the ammoniated pretreatment of rice straw with ammonium carbonate, with the surface structure becoming rough and loose and the cellulose exposed, which was more favorable to the action of cellulase. In addition, FTIR revealed that the absorption peaks of maize straw at 2920 and 1650 cm−1 were weakened after ammoniation treatment, and the reduction of their peaks represented to some extent the destruction of the lignin structure. Overall, the enzymatic digestion of maize stover was more favorable after the ammoniated pretreatment of maize stover with ammonium carbonate. |
| first_indexed | 2024-03-12T13:34:20Z |
| format | Article |
| id | doaj.art-5fb38e19076142b7b7cda3fe2b14283a |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-12T13:34:20Z |
| publishDate | 2023-06-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-5fb38e19076142b7b7cda3fe2b14283a2023-08-24T06:00:59ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-06-01441221622210.13386/j.issn1002-0306.20220901552022090155-12Effect of Ammonia Pretreatment on Enzymatic Sugar Production from Corn StoverJianxun LI0Xin LI1Yumeng WANG2Wenjuan ZHANG3Jiping WEI4Wenjun SONG5College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaCollege of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaCollege of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaCollege of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaCollege of Biotechnology, Tianjin Modern Vocational and Technical College, Tianjin 300350, ChinaCollege of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaTo make comprehensive use of maize stover, cellulase degradation of maize stover was accelerated. In this paper, maize straw was used as raw material, and reducing sugar yield was the main indicator. The reducing sugar yield was determined by the DNS method after enzymatic digestion of maize straw by ammonification pretreatment, and the effects of ammonification agent type, concentration, solid content and ammonification time on the enzymatic sugar production from maize straw were investigated. The results showed that the highest reducing sugar yield of 314.18 mg/mL was achieved under this condition with ammonium carbonate as the ammoniating agent, ammoniating agent concentration of 20%, solid content of 50% and ammoniating time of 11 d, which was 51.80% higher compared to direct enzymatic digestion of the straw. In addition, it was found by SEM that the structure of lignin and cellulose changed after the ammoniated pretreatment of rice straw with ammonium carbonate, with the surface structure becoming rough and loose and the cellulose exposed, which was more favorable to the action of cellulase. In addition, FTIR revealed that the absorption peaks of maize straw at 2920 and 1650 cm−1 were weakened after ammoniation treatment, and the reduction of their peaks represented to some extent the destruction of the lignin structure. Overall, the enzymatic digestion of maize stover was more favorable after the ammoniated pretreatment of maize stover with ammonium carbonate.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022090155maize stoverlignincelluloseammoniated pretreatmentcellulase |
| spellingShingle | Jianxun LI Xin LI Yumeng WANG Wenjuan ZHANG Jiping WEI Wenjun SONG Effect of Ammonia Pretreatment on Enzymatic Sugar Production from Corn Stover Shipin gongye ke-ji maize stover lignin cellulose ammoniated pretreatment cellulase |
| title | Effect of Ammonia Pretreatment on Enzymatic Sugar Production from Corn Stover |
| title_full | Effect of Ammonia Pretreatment on Enzymatic Sugar Production from Corn Stover |
| title_fullStr | Effect of Ammonia Pretreatment on Enzymatic Sugar Production from Corn Stover |
| title_full_unstemmed | Effect of Ammonia Pretreatment on Enzymatic Sugar Production from Corn Stover |
| title_short | Effect of Ammonia Pretreatment on Enzymatic Sugar Production from Corn Stover |
| title_sort | effect of ammonia pretreatment on enzymatic sugar production from corn stover |
| topic | maize stover lignin cellulose ammoniated pretreatment cellulase |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022090155 |
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