LC/DAD determination of biogenic amines in serum of patients with diabetes mellitus, chronic urticaria or Hashimoto's thyroiditis
Biogenic amines are integral part of nearly every cell. In present study, we used method of acidic extraction of histamine (His), of polyamines putrescine (Put), spermidine (Spd) and catecholamines epinephrine (Epi) and norepinephrine (NE) from human serum; precolumn derivatization with dan...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Serbian Chemical Society
2016-01-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2016/0352-51391600020T.pdf |
| Summary: | Biogenic amines are integral part of nearly every cell. In present study, we
used method of acidic extraction of histamine (His), of polyamines putrescine
(Put), spermidine (Spd) and catecholamines epinephrine (Epi) and
norepinephrine (NE) from human serum; precolumn derivatization with dansyl
chloride, and LC/DAD analysis of the biogenic amines, in aim to monitor
differences of their levels in patients with diabetes mellitus, chronic
urticaria, and Hashimoto's thyroiditis, compared to healthy subjects, and to
observe them as possible markers for immune mediated diseases. Method of
retention times was used for determination of serum biogenic amines. We found
statistically significant differences in putrescine and histamine levels in
diabetes mellitus patients; putrescine, histamine, spermidine and epinephrine
levels in chronic urticaria patients compared to healthy controls, and
putrescine and spermidine levels in Hashimoto's thyroiditis patients,
compared to controls. Norepinephrine was found only in serum of patients with
chronic urticaria. The values of recovery, evaluated in controls, varied
between 85.7% and 106.7%. The statistically significant changes in
putrescine, histamine, spermidine and epinephrine levels in patients compared
to healthy people reflects the existence of biochemical disturbances in
mentioned immune-mediated diseases. |
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| ISSN: | 0352-5139 1820-7421 |