Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications
A functional interpretation of filtered candidates and predicted regulatory pathways related to cashmere growth from sequencing trials needs available cell models, especially for hair matrix cells (HMCs), whose continual proliferation and differentiation result in rapid hair growth. To fulfill such...
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MDPI AG
2020-08-01
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| author | Sen Ma Lamei Wang Bo Zong Ying Wang Xiaolong Wang Yinghua Shi Yuxin Yang Yulin Chen |
| author_facet | Sen Ma Lamei Wang Bo Zong Ying Wang Xiaolong Wang Yinghua Shi Yuxin Yang Yulin Chen |
| author_sort | Sen Ma |
| collection | DOAJ |
| description | A functional interpretation of filtered candidates and predicted regulatory pathways related to cashmere growth from sequencing trials needs available cell models, especially for hair matrix cells (HMCs), whose continual proliferation and differentiation result in rapid hair growth. To fulfill such goals, we herein obtained primary goat HMCs via a microdissection-based method; optimized the selection of the culture medium and coating substances for better cell maintenance; and exemplified their usefulness through examining the effects of calcium and all-trans retinoic acid (ATRA) on cells using immunoblotting, flow cytometry, and other techniques. As a result, we successfully acquired primary and passaged goat HMCs with typical keratinocyte morphology. Calcium-free RPMI (Roswell Park Memorial Institute) 1640 and MEM (minimum Eagle’s medium) outperformed normal DMEM/F12 (Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12) on long-term cell maintenance, whereas serum-free media K-SFM and EpiLife failed to support cell growth. HMCs differed molecularly and morphologically from their neighbor dermal papilla cells on expressions of feature genes, such as <i>HOXC13</i>, and on characteristic keratinocyte-like appearances versus fibroblast shapes, respectively. Higher calcium concentrations significantly stimulated the expression of the genes (e.g., <i>KRT1</i> and <i>IVL</i>) involved in keratinocyte differentiation and, promoted cell proliferation. Moreover, 10<sup>−5</sup> M ATRA obviously boosted goat HMC expansions and changed their cell cycle distributions compared to the controls. Our study shines a light on researches exploring the mechanisms underlying the growth of cashmere. |
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| series | Animals |
| spelling | doaj.art-5fb78bb30baa4c3dad04449ab221ad102023-11-20T09:51:24ZengMDPI AGAnimals2076-26152020-08-01108140010.3390/ani10081400Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified ApplicationsSen Ma0Lamei Wang1Bo Zong2Ying Wang3Xiaolong Wang4Yinghua Shi5Yuxin Yang6Yulin Chen7College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaA functional interpretation of filtered candidates and predicted regulatory pathways related to cashmere growth from sequencing trials needs available cell models, especially for hair matrix cells (HMCs), whose continual proliferation and differentiation result in rapid hair growth. To fulfill such goals, we herein obtained primary goat HMCs via a microdissection-based method; optimized the selection of the culture medium and coating substances for better cell maintenance; and exemplified their usefulness through examining the effects of calcium and all-trans retinoic acid (ATRA) on cells using immunoblotting, flow cytometry, and other techniques. As a result, we successfully acquired primary and passaged goat HMCs with typical keratinocyte morphology. Calcium-free RPMI (Roswell Park Memorial Institute) 1640 and MEM (minimum Eagle’s medium) outperformed normal DMEM/F12 (Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12) on long-term cell maintenance, whereas serum-free media K-SFM and EpiLife failed to support cell growth. HMCs differed molecularly and morphologically from their neighbor dermal papilla cells on expressions of feature genes, such as <i>HOXC13</i>, and on characteristic keratinocyte-like appearances versus fibroblast shapes, respectively. Higher calcium concentrations significantly stimulated the expression of the genes (e.g., <i>KRT1</i> and <i>IVL</i>) involved in keratinocyte differentiation and, promoted cell proliferation. Moreover, 10<sup>−5</sup> M ATRA obviously boosted goat HMC expansions and changed their cell cycle distributions compared to the controls. Our study shines a light on researches exploring the mechanisms underlying the growth of cashmere.https://www.mdpi.com/2076-2615/10/8/1400hair folliclehair growthcalciumall-trans retinoic acidHMCsDPCs |
| spellingShingle | Sen Ma Lamei Wang Bo Zong Ying Wang Xiaolong Wang Yinghua Shi Yuxin Yang Yulin Chen Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications Animals hair follicle hair growth calcium all-trans retinoic acid HMCs DPCs |
| title | Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications |
| title_full | Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications |
| title_fullStr | Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications |
| title_full_unstemmed | Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications |
| title_short | Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications |
| title_sort | cultivation of hair matrix cells from cashmere goat skins and exemplified applications |
| topic | hair follicle hair growth calcium all-trans retinoic acid HMCs DPCs |
| url | https://www.mdpi.com/2076-2615/10/8/1400 |
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