Exogenous Trehalose Largely Alleviates Ionic Unbalance, ROS Burst and PCD Occurrence Induced by High Salinity in Arabidopsis Seedlings
Trehalose (Tre) has been reported to play a critical role in plant response to salinity and the involved mechanisms remain to be investigated in detail. Here, the putative roles of Tre in regulation of ionic balance, cellular redox state, cell death were studied in Arabidopsis under high salt condit...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00570/full |
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| author | Lei eYang Xiaoju eZhao Matthew ePaul Hong eZhu Yuangang eZu Zhonghua eTang |
| author_facet | Lei eYang Xiaoju eZhao Matthew ePaul Hong eZhu Yuangang eZu Zhonghua eTang |
| author_sort | Lei eYang |
| collection | DOAJ |
| description | Trehalose (Tre) has been reported to play a critical role in plant response to salinity and the involved mechanisms remain to be investigated in detail. Here, the putative roles of Tre in regulation of ionic balance, cellular redox state, cell death were studied in Arabidopsis under high salt condition. Our results found that the salt-induced restrictions on both vegetative and reproductive growth in salt-stressed plants were largely alleviated by exogenous supply with Tre. The microprobe analysis of ionic dynamics in the leaf and stem of florescence highlighted the Tre ability to retain K and K/Na ratio in plant tissues to improve salt tolerance. The flow cytometric (FCM) assay of cellular levels of ROS (reactive oxygen species) and PCD (programmed cell death) displayed that Tre was able to antagonized salt-induced damages in redox state and cell death and sucrose did not play the same role with Tre. By comparing ionic distribution in leaf and IS (inflorescence stem), we found that Tre was able to restrict Na transportation to IS from leaves since that the ratio of Na accumulation in leaves relative to IS was largely improved due to Tre. The marked decrease of Na ion and improved sucrose level in IS might account for the promoted floral growth when Tre was included in the saline solution. At the same time, endogenous soluble sugars and antioxidant enzyme activities in the salt-stressed plants were also elevated by Tre to counteract high salt stress. We concluded that Tre could improve Arabidopsis salt resistance with respect to biomass accumulation and floral transition in the means of regulating plant redox state, cell death and ionic distribution. |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-14T00:08:40Z |
| publishDate | 2014-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-5fb7e1a72bb948a2be2ab774922bec8f2022-12-22T02:23:27ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2014-10-01510.3389/fpls.2014.0057083253Exogenous Trehalose Largely Alleviates Ionic Unbalance, ROS Burst and PCD Occurrence Induced by High Salinity in Arabidopsis SeedlingsLei eYang0Xiaoju eZhao1Matthew ePaul2Hong eZhu3Yuangang eZu4Zhonghua eTang5Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai Chenshan Botanical Garden, Shanghai, ChinaDaqing Normal UniversityPlant Biology and Crop Science, Rothamsted Research, Harpenden, Hertfordshire, UKThe key Laboratory of Plant Ecology, Northeast Forestry UniversityThe key Laboratory of Plant Ecology, Northeast Forestry UniversityThe key Laboratory of Plant Ecology, Northeast Forestry UniversityTrehalose (Tre) has been reported to play a critical role in plant response to salinity and the involved mechanisms remain to be investigated in detail. Here, the putative roles of Tre in regulation of ionic balance, cellular redox state, cell death were studied in Arabidopsis under high salt condition. Our results found that the salt-induced restrictions on both vegetative and reproductive growth in salt-stressed plants were largely alleviated by exogenous supply with Tre. The microprobe analysis of ionic dynamics in the leaf and stem of florescence highlighted the Tre ability to retain K and K/Na ratio in plant tissues to improve salt tolerance. The flow cytometric (FCM) assay of cellular levels of ROS (reactive oxygen species) and PCD (programmed cell death) displayed that Tre was able to antagonized salt-induced damages in redox state and cell death and sucrose did not play the same role with Tre. By comparing ionic distribution in leaf and IS (inflorescence stem), we found that Tre was able to restrict Na transportation to IS from leaves since that the ratio of Na accumulation in leaves relative to IS was largely improved due to Tre. The marked decrease of Na ion and improved sucrose level in IS might account for the promoted floral growth when Tre was included in the saline solution. At the same time, endogenous soluble sugars and antioxidant enzyme activities in the salt-stressed plants were also elevated by Tre to counteract high salt stress. We concluded that Tre could improve Arabidopsis salt resistance with respect to biomass accumulation and floral transition in the means of regulating plant redox state, cell death and ionic distribution.http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00570/fullArabidopsisTrehaloseion homeostasissalt stressredox state |
| spellingShingle | Lei eYang Xiaoju eZhao Matthew ePaul Hong eZhu Yuangang eZu Zhonghua eTang Exogenous Trehalose Largely Alleviates Ionic Unbalance, ROS Burst and PCD Occurrence Induced by High Salinity in Arabidopsis Seedlings Frontiers in Plant Science Arabidopsis Trehalose ion homeostasis salt stress redox state |
| title | Exogenous Trehalose Largely Alleviates Ionic Unbalance, ROS Burst and PCD Occurrence Induced by High Salinity in Arabidopsis Seedlings |
| title_full | Exogenous Trehalose Largely Alleviates Ionic Unbalance, ROS Burst and PCD Occurrence Induced by High Salinity in Arabidopsis Seedlings |
| title_fullStr | Exogenous Trehalose Largely Alleviates Ionic Unbalance, ROS Burst and PCD Occurrence Induced by High Salinity in Arabidopsis Seedlings |
| title_full_unstemmed | Exogenous Trehalose Largely Alleviates Ionic Unbalance, ROS Burst and PCD Occurrence Induced by High Salinity in Arabidopsis Seedlings |
| title_short | Exogenous Trehalose Largely Alleviates Ionic Unbalance, ROS Burst and PCD Occurrence Induced by High Salinity in Arabidopsis Seedlings |
| title_sort | exogenous trehalose largely alleviates ionic unbalance ros burst and pcd occurrence induced by high salinity in arabidopsis seedlings |
| topic | Arabidopsis Trehalose ion homeostasis salt stress redox state |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00570/full |
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