Assessment of genetic diversity in Iranian wheat (Triticum aestivum L.) cultivars and lines using microsatellite markers
In this study, genetic diversity of 20 wheat genotypes was evaluated using 126 simple sequence repeats (SSR) alleles, covering all three wheat genomes. A total of 1557 allelic variants were detected for 126 SSR loci. The number of alleles per locus ranged from 4 to 19 and the allelic polymorphism in...
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| Format: | Article |
| Language: | English |
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Genetics and Agricultural Biotechnology Institute of Tabarestan
2014-06-01
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| Series: | Journal of Plant Molecular Breeding |
| Subjects: | |
| Online Access: | http://www.jpmb-gabit.ir/article_8430_97b728e1ae5d70418bb6cbac76e615de.pdf |
| _version_ | 1818022110712299520 |
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| author | Robabeh Mollaheydari Bafghi Amin Baghizadeh Ghasem Mohammadi-Nejad Babak Nakhoda |
| author_facet | Robabeh Mollaheydari Bafghi Amin Baghizadeh Ghasem Mohammadi-Nejad Babak Nakhoda |
| author_sort | Robabeh Mollaheydari Bafghi |
| collection | DOAJ |
| description | In this study, genetic diversity of 20 wheat genotypes was evaluated using 126 simple sequence repeats (SSR) alleles, covering all three wheat genomes. A total of 1557 allelic variants were detected for 126 SSR loci. The number of alleles per locus ranged from 4 to 19 and the allelic polymorphism information content (PIC) varied from 0.66 (<em>Xgwm</em>429) to 0.94 (<em>Xgwm</em>212 and<em> Xgwm</em>515). The highest polymorphism was observed in <em>Xgwm212</em> and <em>Xgwm515 </em>primerswith 19 alleles, while the lowest polymorphism belonged to <em>Xgwm429</em> with 4 alleles. The highest number of alleles per locus was detected in the genome A with 594 , compared to 552 and 411 for B and D genomes, respectively. Dendrogram was constructed using Dice similarity coefficient and UPGMA algorithm by NTSYSpc2.0 software and genotypes were grouped in to six clusters. The knowledge about the genetic relationships of genotypes provides useful information to address breeding programs and germplasm resource management. This study also confirms the usefulness of SSR markers to study wheat genetic diversity. |
| first_indexed | 2024-04-14T08:27:59Z |
| format | Article |
| id | doaj.art-5fb878c72266414ab4ea7ab80dc3be48 |
| institution | Directory Open Access Journal |
| issn | 2322-3332 2322-5092 |
| language | English |
| last_indexed | 2024-04-14T08:27:59Z |
| publishDate | 2014-06-01 |
| publisher | Genetics and Agricultural Biotechnology Institute of Tabarestan |
| record_format | Article |
| series | Journal of Plant Molecular Breeding |
| spelling | doaj.art-5fb878c72266414ab4ea7ab80dc3be482022-12-22T02:04:00ZengGenetics and Agricultural Biotechnology Institute of TabarestanJournal of Plant Molecular Breeding2322-33322322-50922014-06-0121748910.22058/jpmb.2014.84308430Assessment of genetic diversity in Iranian wheat (Triticum aestivum L.) cultivars and lines using microsatellite markersRobabeh Mollaheydari Bafghi0Amin Baghizadeh1Ghasem Mohammadi-Nejad2Babak Nakhoda3Department of Plant Breeding, Graduate University of Advanced Technology.Department of Biotechnology, Institute of Sciences and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman-Iran.Department of Agronomy and plant Breeding, College of Agriculture and Center of Excellence for Abiotic Stress in Cereal Crop., Shahid Bahonar University of Kerman-Iran.Department of System Biology , Agricultural Biotechnology Research Institute of Iran , P. O. Box, 31535-1897, Karaj –Iran.In this study, genetic diversity of 20 wheat genotypes was evaluated using 126 simple sequence repeats (SSR) alleles, covering all three wheat genomes. A total of 1557 allelic variants were detected for 126 SSR loci. The number of alleles per locus ranged from 4 to 19 and the allelic polymorphism information content (PIC) varied from 0.66 (<em>Xgwm</em>429) to 0.94 (<em>Xgwm</em>212 and<em> Xgwm</em>515). The highest polymorphism was observed in <em>Xgwm212</em> and <em>Xgwm515 </em>primerswith 19 alleles, while the lowest polymorphism belonged to <em>Xgwm429</em> with 4 alleles. The highest number of alleles per locus was detected in the genome A with 594 , compared to 552 and 411 for B and D genomes, respectively. Dendrogram was constructed using Dice similarity coefficient and UPGMA algorithm by NTSYSpc2.0 software and genotypes were grouped in to six clusters. The knowledge about the genetic relationships of genotypes provides useful information to address breeding programs and germplasm resource management. This study also confirms the usefulness of SSR markers to study wheat genetic diversity.http://www.jpmb-gabit.ir/article_8430_97b728e1ae5d70418bb6cbac76e615de.pdfGenetic diversityMicrosatellite markersPolymorphismWheat (Triticum aestivum L.) |
| spellingShingle | Robabeh Mollaheydari Bafghi Amin Baghizadeh Ghasem Mohammadi-Nejad Babak Nakhoda Assessment of genetic diversity in Iranian wheat (Triticum aestivum L.) cultivars and lines using microsatellite markers Journal of Plant Molecular Breeding Genetic diversity Microsatellite markers Polymorphism Wheat (Triticum aestivum L.) |
| title | Assessment of genetic diversity in Iranian wheat (Triticum aestivum L.) cultivars and lines using microsatellite markers |
| title_full | Assessment of genetic diversity in Iranian wheat (Triticum aestivum L.) cultivars and lines using microsatellite markers |
| title_fullStr | Assessment of genetic diversity in Iranian wheat (Triticum aestivum L.) cultivars and lines using microsatellite markers |
| title_full_unstemmed | Assessment of genetic diversity in Iranian wheat (Triticum aestivum L.) cultivars and lines using microsatellite markers |
| title_short | Assessment of genetic diversity in Iranian wheat (Triticum aestivum L.) cultivars and lines using microsatellite markers |
| title_sort | assessment of genetic diversity in iranian wheat triticum aestivum l cultivars and lines using microsatellite markers |
| topic | Genetic diversity Microsatellite markers Polymorphism Wheat (Triticum aestivum L.) |
| url | http://www.jpmb-gabit.ir/article_8430_97b728e1ae5d70418bb6cbac76e615de.pdf |
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