Determination the causative strain for hydatid cyst in Iraqi cattle by using ND1 gene
Hydatid Cysts were obtained from 15 cows from liver, lung, spleen, heart, and peritoneal cavity, between December 2014 and October 2015. Hydatid cysts (protoscoleces) were used for deoxyribonucleic acid extraction by using mechanical grinder. The purification of mtDNA was done by (promega kit, USA)....
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| Format: | Article |
| Language: | English |
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University of Baghdad, College of Veterinary Medicine
2017-06-01
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| Series: | The Iraqi Journal of Veterinary Medicine |
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| Online Access: | https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/71 |
| _version_ | 1818892373183168512 |
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| author | Mohammed J. Muhaidi |
| author_facet | Mohammed J. Muhaidi |
| author_sort | Mohammed J. Muhaidi |
| collection | DOAJ |
| description | Hydatid Cysts were obtained from 15 cows from liver, lung, spleen, heart, and peritoneal cavity, between December 2014 and October 2015. Hydatid cysts (protoscoleces) were used for deoxyribonucleic acid extraction by using mechanical grinder. The purification of mtDNA was done by (promega kit, USA). The mitochondrial NADH dehydrogenase subunit 1 (ND1) genes was used as targets for polymerase chain reaction amplification, all hydatid cysts yielded amplification products. Polymerase chain reaction product for NADH1 800 basic pair. The polymerase chain reaction products were purified and partial sequences were generated. The sequences obtained were found to align with corresponding region for ND1 gene in the Gene Bank nucleotide database confirming to genotype of sheep strain (G1) in Iraq, Phylogenetic analysis of partial sequence data from ND1 genes for obtained Phylogenetic tree. G1 genotype was the most common taxon and the actual source of infection of Iraqi's cattle. All of 15 strains were G1 genotype (sheep strain) based on the partial sequences of NADH dehydrogenase 1 (ND1). |
| first_indexed | 2024-12-19T17:55:40Z |
| format | Article |
| id | doaj.art-5fbee551671c4aecb75150d0821d7662 |
| institution | Directory Open Access Journal |
| issn | 1609-5693 2410-7409 |
| language | English |
| last_indexed | 2024-12-19T17:55:40Z |
| publishDate | 2017-06-01 |
| publisher | University of Baghdad, College of Veterinary Medicine |
| record_format | Article |
| series | The Iraqi Journal of Veterinary Medicine |
| spelling | doaj.art-5fbee551671c4aecb75150d0821d76622022-12-21T20:11:49ZengUniversity of Baghdad, College of Veterinary MedicineThe Iraqi Journal of Veterinary Medicine1609-56932410-74092017-06-0141110.30539/iraqijvm.v41i1.71Determination the causative strain for hydatid cyst in Iraqi cattle by using ND1 geneMohammed J. Muhaidi01College of Veterinary Medicine, Al-Fallujah University, 2College of Medicine, Al- Anbar University, Iraq.Hydatid Cysts were obtained from 15 cows from liver, lung, spleen, heart, and peritoneal cavity, between December 2014 and October 2015. Hydatid cysts (protoscoleces) were used for deoxyribonucleic acid extraction by using mechanical grinder. The purification of mtDNA was done by (promega kit, USA). The mitochondrial NADH dehydrogenase subunit 1 (ND1) genes was used as targets for polymerase chain reaction amplification, all hydatid cysts yielded amplification products. Polymerase chain reaction product for NADH1 800 basic pair. The polymerase chain reaction products were purified and partial sequences were generated. The sequences obtained were found to align with corresponding region for ND1 gene in the Gene Bank nucleotide database confirming to genotype of sheep strain (G1) in Iraq, Phylogenetic analysis of partial sequence data from ND1 genes for obtained Phylogenetic tree. G1 genotype was the most common taxon and the actual source of infection of Iraqi's cattle. All of 15 strains were G1 genotype (sheep strain) based on the partial sequences of NADH dehydrogenase 1 (ND1).https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/71G1 strain, Hydatid cyst, ND1 gene, Cattle, Sequences |
| spellingShingle | Mohammed J. Muhaidi Determination the causative strain for hydatid cyst in Iraqi cattle by using ND1 gene The Iraqi Journal of Veterinary Medicine G1 strain, Hydatid cyst, ND1 gene, Cattle, Sequences |
| title | Determination the causative strain for hydatid cyst in Iraqi cattle by using ND1 gene |
| title_full | Determination the causative strain for hydatid cyst in Iraqi cattle by using ND1 gene |
| title_fullStr | Determination the causative strain for hydatid cyst in Iraqi cattle by using ND1 gene |
| title_full_unstemmed | Determination the causative strain for hydatid cyst in Iraqi cattle by using ND1 gene |
| title_short | Determination the causative strain for hydatid cyst in Iraqi cattle by using ND1 gene |
| title_sort | determination the causative strain for hydatid cyst in iraqi cattle by using nd1 gene |
| topic | G1 strain, Hydatid cyst, ND1 gene, Cattle, Sequences |
| url | https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/71 |
| work_keys_str_mv | AT mohammedjmuhaidi determinationthecausativestrainforhydatidcystiniraqicattlebyusingnd1gene |