Polymorphisms of FST gene and their association with wool quality traits in Chinese Merino sheep.
Follistatin (FST) is involved in hair follicle morphogenesis. However, its effects on hair traits are not clear. This study was designed to investigate the effects of FST gene single nucleotide polymorphisms (SNP) on wool quality traits in Chinese Merino sheep (Junken Type). We performed gene expres...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5383234?pdf=render |
| _version_ | 1828760091533246464 |
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| author | Guang-Wei Ma Yan-Kai Chu Wen-Jian Zhang Fei-Yue Qin Song-Song Xu Hua Yang En-Guang Rong Zhi-Qiang Du Shou-Zhi Wang Hui Li Ning Wang |
| author_facet | Guang-Wei Ma Yan-Kai Chu Wen-Jian Zhang Fei-Yue Qin Song-Song Xu Hua Yang En-Guang Rong Zhi-Qiang Du Shou-Zhi Wang Hui Li Ning Wang |
| author_sort | Guang-Wei Ma |
| collection | DOAJ |
| description | Follistatin (FST) is involved in hair follicle morphogenesis. However, its effects on hair traits are not clear. This study was designed to investigate the effects of FST gene single nucleotide polymorphisms (SNP) on wool quality traits in Chinese Merino sheep (Junken Type). We performed gene expression analysis, SNP detection, and association analysis of FST gene with sheep wool quality traits. The real-time RT-PCR analysis showed that FST gene was differentially expressed in adult skin between Chinese Merino sheep (Junken Type) and Suffolk sheep. Immunostaining showed that FST was localized in inner root sheath (IRS) and matrix of hair follicle (HF) in both SF and Suffolk sheep. Sequencing analysis identified a total of seven SNPs (termed SNPs 1-7) in the FST gene in Chinese Merino sheep (Junken Type). Association analysis showed that SNP2 (Chr 16. 25,633,662 G>A) was significantly associated with average wool fiber diameter, wool fineness SD, and wool crimp (P < 0.05). SNP4 (Chr 16. 25,633,569 C>T) was significantly associated with wool fineness SD and CV of fiber diameter (P < 0.05). Similarly, the haplotypes derived from these seven identified SNPs were also significantly associated with average wool fiber diameter, wool fineness SD, CV of fiber diameter, and wool crimp (P < 0.05). Our results suggest that FST influences wool quality traits and its SNPs 2 and 4 might be useful markers for marker-assisted selection and sheep breeding. |
| first_indexed | 2024-12-11T01:07:54Z |
| format | Article |
| id | doaj.art-5fbff37a2a4646afb675031aa60ca975 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T01:07:54Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5fbff37a2a4646afb675031aa60ca9752022-12-22T01:26:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01124e017486810.1371/journal.pone.0174868Polymorphisms of FST gene and their association with wool quality traits in Chinese Merino sheep.Guang-Wei MaYan-Kai ChuWen-Jian ZhangFei-Yue QinSong-Song XuHua YangEn-Guang RongZhi-Qiang DuShou-Zhi WangHui LiNing WangFollistatin (FST) is involved in hair follicle morphogenesis. However, its effects on hair traits are not clear. This study was designed to investigate the effects of FST gene single nucleotide polymorphisms (SNP) on wool quality traits in Chinese Merino sheep (Junken Type). We performed gene expression analysis, SNP detection, and association analysis of FST gene with sheep wool quality traits. The real-time RT-PCR analysis showed that FST gene was differentially expressed in adult skin between Chinese Merino sheep (Junken Type) and Suffolk sheep. Immunostaining showed that FST was localized in inner root sheath (IRS) and matrix of hair follicle (HF) in both SF and Suffolk sheep. Sequencing analysis identified a total of seven SNPs (termed SNPs 1-7) in the FST gene in Chinese Merino sheep (Junken Type). Association analysis showed that SNP2 (Chr 16. 25,633,662 G>A) was significantly associated with average wool fiber diameter, wool fineness SD, and wool crimp (P < 0.05). SNP4 (Chr 16. 25,633,569 C>T) was significantly associated with wool fineness SD and CV of fiber diameter (P < 0.05). Similarly, the haplotypes derived from these seven identified SNPs were also significantly associated with average wool fiber diameter, wool fineness SD, CV of fiber diameter, and wool crimp (P < 0.05). Our results suggest that FST influences wool quality traits and its SNPs 2 and 4 might be useful markers for marker-assisted selection and sheep breeding.http://europepmc.org/articles/PMC5383234?pdf=render |
| spellingShingle | Guang-Wei Ma Yan-Kai Chu Wen-Jian Zhang Fei-Yue Qin Song-Song Xu Hua Yang En-Guang Rong Zhi-Qiang Du Shou-Zhi Wang Hui Li Ning Wang Polymorphisms of FST gene and their association with wool quality traits in Chinese Merino sheep. PLoS ONE |
| title | Polymorphisms of FST gene and their association with wool quality traits in Chinese Merino sheep. |
| title_full | Polymorphisms of FST gene and their association with wool quality traits in Chinese Merino sheep. |
| title_fullStr | Polymorphisms of FST gene and their association with wool quality traits in Chinese Merino sheep. |
| title_full_unstemmed | Polymorphisms of FST gene and their association with wool quality traits in Chinese Merino sheep. |
| title_short | Polymorphisms of FST gene and their association with wool quality traits in Chinese Merino sheep. |
| title_sort | polymorphisms of fst gene and their association with wool quality traits in chinese merino sheep |
| url | http://europepmc.org/articles/PMC5383234?pdf=render |
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