Methodological validation of measuring Hanwoo hair cortisol concentration using bead beater and surgical scissors
Different methodologies in hair cortisol extraction may alter the final output. Thus finding the standard methodology according to a laboratory facilities is pivotal. This study was carried out to validate the feasibility of two methods of...
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| Format: | Article |
| Language: | English |
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Korean Society of Animal Sciences and Technology
2019-01-01
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| Series: | Journal of Animal Science and Technology |
| Subjects: | |
| Online Access: | http://www.ejast.org/archive/view_article?pid=jast-61-1-41 |
| _version_ | 1819211754555572224 |
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| author | Jalil Ghassemi Nejad Mohammad Ataallahi Kyu-Hyun Park |
| author_facet | Jalil Ghassemi Nejad Mohammad Ataallahi Kyu-Hyun Park |
| author_sort | Jalil Ghassemi Nejad |
| collection | DOAJ |
| description | Different methodologies in hair cortisol extraction may alter the final output.
Thus finding the standard methodology according to a laboratory facilities is
pivotal. This study was carried out to validate the feasibility of two methods
of grinding hair for cortisol extraction in Korean native (Hanwoo) cattle. Hair
from nine cattle including mature cows, heifers, and calves were assigned to one
of the following methods for grinding hair; 1) using bead beater (BB) and 2)
using surgical scissors (SS). Hair samples (> 1 g) were harvested from
forehead of each individual twice (first and second measurement) to validate the
results. To improve the accuracy of the obtained data, each sample was
duplicated into two wells during enzyme immunoassay (EIA) analysis. Overall
comparison of hair cortisol concentration (HCC) showed that the data within the
range (out of the range) of standards provided by the EIA kit were 88.9%
(11.1%) and 66.7% (33.3%) for BB compared with SS,
respectively. In the first measurement, application of BB was tended to show
higher (p = 0.056) amount of HCC compared with SS. In
the second measurement application of BB showed higher (p
= 0.0028) amount of HCC compared with SS. Among the cattle, calves showed
higher HCC using BB compared with SS (p < 0.05).
Application of BB in hair grinding methodology for Hanwoo cattle may improve
cortisol extraction in comparison to application of SS method, with more
consistency. Thus, it would be the preferable method to use. |
| first_indexed | 2024-12-23T06:32:06Z |
| format | Article |
| id | doaj.art-5fc23c06665a46d48d988a2e880a6632 |
| institution | Directory Open Access Journal |
| issn | 1598-9429 2055-0391 |
| language | English |
| last_indexed | 2024-12-23T06:32:06Z |
| publishDate | 2019-01-01 |
| publisher | Korean Society of Animal Sciences and Technology |
| record_format | Article |
| series | Journal of Animal Science and Technology |
| spelling | doaj.art-5fc23c06665a46d48d988a2e880a66322022-12-21T17:56:54ZengKorean Society of Animal Sciences and TechnologyJournal of Animal Science and Technology1598-94292055-03912019-01-01611414610.5187/jast.2019.61.1.41jast-61-1-41Methodological validation of measuring Hanwoo hair cortisol concentration using bead beater and surgical scissorsJalil Ghassemi Nejad0Mohammad Ataallahi1Kyu-Hyun Park2College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, KoreaCollege of Animal Life Sciences, Kangwon National University, Chuncheon 24341, KoreaCollege of Animal Life Sciences, Kangwon National University, Chuncheon 24341, KoreaDifferent methodologies in hair cortisol extraction may alter the final output. Thus finding the standard methodology according to a laboratory facilities is pivotal. This study was carried out to validate the feasibility of two methods of grinding hair for cortisol extraction in Korean native (Hanwoo) cattle. Hair from nine cattle including mature cows, heifers, and calves were assigned to one of the following methods for grinding hair; 1) using bead beater (BB) and 2) using surgical scissors (SS). Hair samples (> 1 g) were harvested from forehead of each individual twice (first and second measurement) to validate the results. To improve the accuracy of the obtained data, each sample was duplicated into two wells during enzyme immunoassay (EIA) analysis. Overall comparison of hair cortisol concentration (HCC) showed that the data within the range (out of the range) of standards provided by the EIA kit were 88.9% (11.1%) and 66.7% (33.3%) for BB compared with SS, respectively. In the first measurement, application of BB was tended to show higher (p = 0.056) amount of HCC compared with SS. In the second measurement application of BB showed higher (p = 0.0028) amount of HCC compared with SS. Among the cattle, calves showed higher HCC using BB compared with SS (p < 0.05). Application of BB in hair grinding methodology for Hanwoo cattle may improve cortisol extraction in comparison to application of SS method, with more consistency. Thus, it would be the preferable method to use.http://www.ejast.org/archive/view_article?pid=jast-61-1-41Bead beaterHair cortisol methodologyHanwoo cattleSurgical scissors |
| spellingShingle | Jalil Ghassemi Nejad Mohammad Ataallahi Kyu-Hyun Park Methodological validation of measuring Hanwoo hair cortisol concentration using bead beater and surgical scissors Journal of Animal Science and Technology Bead beater Hair cortisol methodology Hanwoo cattle Surgical scissors |
| title | Methodological validation of measuring Hanwoo hair cortisol
concentration using bead beater and surgical scissors |
| title_full | Methodological validation of measuring Hanwoo hair cortisol
concentration using bead beater and surgical scissors |
| title_fullStr | Methodological validation of measuring Hanwoo hair cortisol
concentration using bead beater and surgical scissors |
| title_full_unstemmed | Methodological validation of measuring Hanwoo hair cortisol
concentration using bead beater and surgical scissors |
| title_short | Methodological validation of measuring Hanwoo hair cortisol
concentration using bead beater and surgical scissors |
| title_sort | methodological validation of measuring hanwoo hair cortisol concentration using bead beater and surgical scissors |
| topic | Bead beater Hair cortisol methodology Hanwoo cattle Surgical scissors |
| url | http://www.ejast.org/archive/view_article?pid=jast-61-1-41 |
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