Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wall
Abstract Microalgae are a renewable and promising biomass for large-scale biofuel, food and nutrient production. However, their efficient exploitation depends on our knowledge of the cell wall composition and organization as it can limit access to high-value molecules. Here we provide an atomic-leve...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-45246-7 |
| _version_ | 1797273952867844096 |
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| author | Alexandre Poulhazan Alexandre A. Arnold Frederic Mentink-Vigier Artur Muszyński Parastoo Azadi Adnan Halim Sergey Y. Vakhrushev Hiren Jitendra Joshi Tuo Wang Dror E. Warschawski Isabelle Marcotte |
| author_facet | Alexandre Poulhazan Alexandre A. Arnold Frederic Mentink-Vigier Artur Muszyński Parastoo Azadi Adnan Halim Sergey Y. Vakhrushev Hiren Jitendra Joshi Tuo Wang Dror E. Warschawski Isabelle Marcotte |
| author_sort | Alexandre Poulhazan |
| collection | DOAJ |
| description | Abstract Microalgae are a renewable and promising biomass for large-scale biofuel, food and nutrient production. However, their efficient exploitation depends on our knowledge of the cell wall composition and organization as it can limit access to high-value molecules. Here we provide an atomic-level model of the non-crystalline and water-insoluble glycoprotein-rich cell wall of Chlamydomonas reinhardtii. Using in situ solid-state and sensitivity-enhanced nuclear magnetic resonance, we reveal unprecedented details on the protein and carbohydrate composition and their nanoscale heterogeneity, as well as the presence of spatially segregated protein- and glycan-rich regions with different dynamics and hydration levels. We show that mannose-rich lower-molecular-weight proteins likely contribute to the cell wall cohesion by binding to high-molecular weight protein components, and that water provides plasticity to the cell-wall architecture. The structural insight exemplifies strategies used by nature to form cell walls devoid of cellulose or other glycan polymers. |
| first_indexed | 2024-03-07T14:51:29Z |
| format | Article |
| id | doaj.art-5fc500f0abfd4dafa265cb16494d2613 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-07T14:51:29Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-5fc500f0abfd4dafa265cb16494d26132024-03-05T19:39:32ZengNature PortfolioNature Communications2041-17232024-02-0115111510.1038/s41467-024-45246-7Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wallAlexandre Poulhazan0Alexandre A. Arnold1Frederic Mentink-Vigier2Artur Muszyński3Parastoo Azadi4Adnan Halim5Sergey Y. Vakhrushev6Hiren Jitendra Joshi7Tuo Wang8Dror E. Warschawski9Isabelle Marcotte10Department of Chemistry, Université du Québec à MontréalDepartment of Chemistry, Université du Québec à MontréalNational High Magnetic Field Laboratory, Florida State UniversityComplex Carbohydrate Research Center, University of GeorgiaComplex Carbohydrate Research Center, University of GeorgiaCopenhagen Center for Glycomics, University of CopenhagenCopenhagen Center for Glycomics, University of CopenhagenCopenhagen Center for Glycomics, University of CopenhagenDepartment of Chemistry, Michigan State UniversityLaboratoire des Biomolécules, LBM, CNRS UMR 7203, Sorbonne Université, École Normale Supérieure, PSL UniversityDepartment of Chemistry, Université du Québec à MontréalAbstract Microalgae are a renewable and promising biomass for large-scale biofuel, food and nutrient production. However, their efficient exploitation depends on our knowledge of the cell wall composition and organization as it can limit access to high-value molecules. Here we provide an atomic-level model of the non-crystalline and water-insoluble glycoprotein-rich cell wall of Chlamydomonas reinhardtii. Using in situ solid-state and sensitivity-enhanced nuclear magnetic resonance, we reveal unprecedented details on the protein and carbohydrate composition and their nanoscale heterogeneity, as well as the presence of spatially segregated protein- and glycan-rich regions with different dynamics and hydration levels. We show that mannose-rich lower-molecular-weight proteins likely contribute to the cell wall cohesion by binding to high-molecular weight protein components, and that water provides plasticity to the cell-wall architecture. The structural insight exemplifies strategies used by nature to form cell walls devoid of cellulose or other glycan polymers.https://doi.org/10.1038/s41467-024-45246-7 |
| spellingShingle | Alexandre Poulhazan Alexandre A. Arnold Frederic Mentink-Vigier Artur Muszyński Parastoo Azadi Adnan Halim Sergey Y. Vakhrushev Hiren Jitendra Joshi Tuo Wang Dror E. Warschawski Isabelle Marcotte Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wall Nature Communications |
| title | Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wall |
| title_full | Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wall |
| title_fullStr | Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wall |
| title_full_unstemmed | Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wall |
| title_short | Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wall |
| title_sort | molecular level architecture of chlamydomonas reinhardtii s glycoprotein rich cell wall |
| url | https://doi.org/10.1038/s41467-024-45246-7 |
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