Dbx1 precursor cells are a source of inspiratory XII premotoneurons
All behaviors require coordinated activation of motoneurons from central command and premotor networks. The genetic identities of premotoneurons providing behaviorally relevant excitation to any pool of respiratory motoneurons remain unknown. Recently, we established in vitro that Dbx1-derived pre-B...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2015-12-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/12301 |
| _version_ | 1811236878697889792 |
|---|---|
| author | Ann L Revill Nikolas C Vann Victoria T Akins Andrew Kottick Paul A Gray Christopher A Del Negro Gregory D Funk |
| author_facet | Ann L Revill Nikolas C Vann Victoria T Akins Andrew Kottick Paul A Gray Christopher A Del Negro Gregory D Funk |
| author_sort | Ann L Revill |
| collection | DOAJ |
| description | All behaviors require coordinated activation of motoneurons from central command and premotor networks. The genetic identities of premotoneurons providing behaviorally relevant excitation to any pool of respiratory motoneurons remain unknown. Recently, we established in vitro that Dbx1-derived pre-Bötzinger complex neurons are critical for rhythm generation and that a subpopulation serves a premotor function (Wang et al., 2014). Here, we further show that a subpopulation of Dbx1-derived intermediate reticular (IRt) neurons are rhythmically active during inspiration and project to the hypoglossal (XII) nucleus that contains motoneurons important for maintaining airway patency. Laser ablation of Dbx1 IRt neurons, 57% of which are glutamatergic, decreased ipsilateral inspiratory motor output without affecting frequency. We conclude that a subset of Dbx1 IRt neurons is a source of premotor excitatory drive, contributing to the inspiratory behavior of XII motoneurons, as well as a key component of the airway control network whose dysfunction contributes to sleep apnea. |
| first_indexed | 2024-04-12T12:15:43Z |
| format | Article |
| id | doaj.art-5fc70366f4a14c048e51751a5098bd05 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:15:43Z |
| publishDate | 2015-12-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-5fc70366f4a14c048e51751a5098bd052022-12-22T03:33:26ZengeLife Sciences Publications LtdeLife2050-084X2015-12-01410.7554/eLife.12301Dbx1 precursor cells are a source of inspiratory XII premotoneuronsAnn L Revill0https://orcid.org/0000-0002-6071-6866Nikolas C Vann1https://orcid.org/0000-0003-4139-0642Victoria T Akins2https://orcid.org/0000-0002-0061-7612Andrew Kottick3https://orcid.org/0000-0002-3731-5140Paul A Gray4Christopher A Del Negro5https://orcid.org/0000-0002-7848-8224Gregory D Funk6https://orcid.org/0000-0001-5848-0631Department of Physiology, Neuroscience and Mental Health Institute, Women and Children's Health Research Institute, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, CanadaDepartment of Applied Science, The College of William and Mary, Williamsburg, United StatesDepartment of Applied Science, The College of William and Mary, Williamsburg, United StatesDepartment of Applied Science, The College of William and Mary, Williamsburg, United StatesDepartment of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, United StatesDepartment of Applied Science, The College of William and Mary, Williamsburg, United StatesDepartment of Physiology, Neuroscience and Mental Health Institute, Women and Children's Health Research Institute, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, CanadaAll behaviors require coordinated activation of motoneurons from central command and premotor networks. The genetic identities of premotoneurons providing behaviorally relevant excitation to any pool of respiratory motoneurons remain unknown. Recently, we established in vitro that Dbx1-derived pre-Bötzinger complex neurons are critical for rhythm generation and that a subpopulation serves a premotor function (Wang et al., 2014). Here, we further show that a subpopulation of Dbx1-derived intermediate reticular (IRt) neurons are rhythmically active during inspiration and project to the hypoglossal (XII) nucleus that contains motoneurons important for maintaining airway patency. Laser ablation of Dbx1 IRt neurons, 57% of which are glutamatergic, decreased ipsilateral inspiratory motor output without affecting frequency. We conclude that a subset of Dbx1 IRt neurons is a source of premotor excitatory drive, contributing to the inspiratory behavior of XII motoneurons, as well as a key component of the airway control network whose dysfunction contributes to sleep apnea.https://elifesciences.org/articles/12301central pattern generatorbreathingmotor behaviorbrainstem |
| spellingShingle | Ann L Revill Nikolas C Vann Victoria T Akins Andrew Kottick Paul A Gray Christopher A Del Negro Gregory D Funk Dbx1 precursor cells are a source of inspiratory XII premotoneurons eLife central pattern generator breathing motor behavior brainstem |
| title | Dbx1 precursor cells are a source of inspiratory XII premotoneurons |
| title_full | Dbx1 precursor cells are a source of inspiratory XII premotoneurons |
| title_fullStr | Dbx1 precursor cells are a source of inspiratory XII premotoneurons |
| title_full_unstemmed | Dbx1 precursor cells are a source of inspiratory XII premotoneurons |
| title_short | Dbx1 precursor cells are a source of inspiratory XII premotoneurons |
| title_sort | dbx1 precursor cells are a source of inspiratory xii premotoneurons |
| topic | central pattern generator breathing motor behavior brainstem |
| url | https://elifesciences.org/articles/12301 |
| work_keys_str_mv | AT annlrevill dbx1precursorcellsareasourceofinspiratoryxiipremotoneurons AT nikolascvann dbx1precursorcellsareasourceofinspiratoryxiipremotoneurons AT victoriatakins dbx1precursorcellsareasourceofinspiratoryxiipremotoneurons AT andrewkottick dbx1precursorcellsareasourceofinspiratoryxiipremotoneurons AT paulagray dbx1precursorcellsareasourceofinspiratoryxiipremotoneurons AT christopheradelnegro dbx1precursorcellsareasourceofinspiratoryxiipremotoneurons AT gregorydfunk dbx1precursorcellsareasourceofinspiratoryxiipremotoneurons |