In Vitro Digestion and Fermentation by Human Fecal Microbiota of Polysaccharides from Flaxseed
The digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions was assessed, as well as in vitro fermentation of FSP by human gut microbiota. FSP was not degraded in the simulated digestive systems (there was no change in molecular weight or content of re...
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MDPI AG
2020-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/19/4354 |
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| author | Xin Zhou Zhao Zhang Fenghong Huang Chen Yang Qingde Huang |
| author_facet | Xin Zhou Zhao Zhang Fenghong Huang Chen Yang Qingde Huang |
| author_sort | Xin Zhou |
| collection | DOAJ |
| description | The digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions was assessed, as well as in vitro fermentation of FSP by human gut microbiota. FSP was not degraded in the simulated digestive systems (there was no change in molecular weight or content of reducing sugars), indicating that ingested FSP would reach the large intestine intact. Changes in carbohydrate content, reducing sugars and culture pH suggested that FSP could be broken down and used by gut microbiota. FSP modulated the composition and structure of the gut microbiota by altering the <i>Firmicutes</i>/<i>Bacteroidetes</i> ratio and increasing the relative abundances of <i>Prevotella</i>, <i>Phascolarctobacterium</i>, <i>Clostridium</i> and <i>Megamonas</i>, which can degrade polysaccharides. Meanwhile, FSP fermentation increased the concentration of short-chain fatty acids, especially propionic and butyric acids. Our results indicate that FSP might be developed as a functional food that benefits gut health. |
| first_indexed | 2024-03-10T16:07:32Z |
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| issn | 1420-3049 |
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| last_indexed | 2024-03-10T16:07:32Z |
| publishDate | 2020-09-01 |
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| series | Molecules |
| spelling | doaj.art-5fc70430e28045d48797f193cd4fc9322023-11-20T14:43:57ZengMDPI AGMolecules1420-30492020-09-012519435410.3390/molecules25194354In Vitro Digestion and Fermentation by Human Fecal Microbiota of Polysaccharides from FlaxseedXin Zhou0Zhao Zhang1Fenghong Huang2Chen Yang3Qingde Huang4Oil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Key Laboratory of Oilseeds Processing, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural affairs, No. 2 Xudong 2nd Road, Wuhan 430062, ChinaOil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Key Laboratory of Oilseeds Processing, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural affairs, No. 2 Xudong 2nd Road, Wuhan 430062, ChinaOil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Key Laboratory of Oilseeds Processing, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural affairs, No. 2 Xudong 2nd Road, Wuhan 430062, ChinaOil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Key Laboratory of Oilseeds Processing, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural affairs, No. 2 Xudong 2nd Road, Wuhan 430062, ChinaOil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Key Laboratory of Oilseeds Processing, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural affairs, No. 2 Xudong 2nd Road, Wuhan 430062, ChinaThe digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions was assessed, as well as in vitro fermentation of FSP by human gut microbiota. FSP was not degraded in the simulated digestive systems (there was no change in molecular weight or content of reducing sugars), indicating that ingested FSP would reach the large intestine intact. Changes in carbohydrate content, reducing sugars and culture pH suggested that FSP could be broken down and used by gut microbiota. FSP modulated the composition and structure of the gut microbiota by altering the <i>Firmicutes</i>/<i>Bacteroidetes</i> ratio and increasing the relative abundances of <i>Prevotella</i>, <i>Phascolarctobacterium</i>, <i>Clostridium</i> and <i>Megamonas</i>, which can degrade polysaccharides. Meanwhile, FSP fermentation increased the concentration of short-chain fatty acids, especially propionic and butyric acids. Our results indicate that FSP might be developed as a functional food that benefits gut health.https://www.mdpi.com/1420-3049/25/19/4354flaxseed polysaccharidessimulated digestiongut microbiotain vitro fermentation |
| spellingShingle | Xin Zhou Zhao Zhang Fenghong Huang Chen Yang Qingde Huang In Vitro Digestion and Fermentation by Human Fecal Microbiota of Polysaccharides from Flaxseed Molecules flaxseed polysaccharides simulated digestion gut microbiota in vitro fermentation |
| title | In Vitro Digestion and Fermentation by Human Fecal Microbiota of Polysaccharides from Flaxseed |
| title_full | In Vitro Digestion and Fermentation by Human Fecal Microbiota of Polysaccharides from Flaxseed |
| title_fullStr | In Vitro Digestion and Fermentation by Human Fecal Microbiota of Polysaccharides from Flaxseed |
| title_full_unstemmed | In Vitro Digestion and Fermentation by Human Fecal Microbiota of Polysaccharides from Flaxseed |
| title_short | In Vitro Digestion and Fermentation by Human Fecal Microbiota of Polysaccharides from Flaxseed |
| title_sort | in vitro digestion and fermentation by human fecal microbiota of polysaccharides from flaxseed |
| topic | flaxseed polysaccharides simulated digestion gut microbiota in vitro fermentation |
| url | https://www.mdpi.com/1420-3049/25/19/4354 |
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