Genomic sequencing confirms absence of introgression despite past hybridisation between a critically endangered bird and its common congener
Genetic swamping resulting from interspecific hybridisation can increase extinction risk for threatened species. The development of high-throughput and reduced-representation genomic sequencing and analyses to generate large numbers of high resolution genomic markers has the potential to reveal intr...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-08-01
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| Series: | Global Ecology and Conservation |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2351989421002316 |
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| author | Natalie J. Forsdick Denise Martini Liz Brown Hugh B. Cross Richard F. Maloney Tammy E. Steeves Michael Knapp |
| author_facet | Natalie J. Forsdick Denise Martini Liz Brown Hugh B. Cross Richard F. Maloney Tammy E. Steeves Michael Knapp |
| author_sort | Natalie J. Forsdick |
| collection | DOAJ |
| description | Genetic swamping resulting from interspecific hybridisation can increase extinction risk for threatened species. The development of high-throughput and reduced-representation genomic sequencing and analyses to generate large numbers of high resolution genomic markers has the potential to reveal introgression previously undetected using small numbers of genetic markers. However, few studies to date have implemented genomic tools to assess the extent of interspecific hybridisation in threatened species. Here we investigate the utility of genome-wide single nucleotide polymorphisms (SNPs) to detect introgression resulting from past interspecific hybridisation in one of the world’s rarest birds. Anthropogenic impacts have resulted in hybridisation and subsequent backcrossing of the critically endangered Aotearoa New Zealand endemic kakī (black stilts; Himantopus novaezelandiae) with the non-threatened self-introduced congeneric poaka (Aotearoa New Zealand population of pied stilts, Himantopus himantopus leucocephalus), yet genetic analyses with a limited set of microsatellite markers revealed no evidence of introgression of poaka genetic material in kakī, excluding one individual. We use genomic data for ~63% of the wild adult kakī population to reassess the extent of introgression resulting from hybridisation between kakī and poaka. Consistent with previous genetic analyses, we detected no introgression from poaka into kakī. These collective results indicate that, for kakī, existing microsatellite markers provide a robust, cost-effective approach to detect cryptic hybrids. Further, for well-differentiated species, the use of genomic markers may not be required to detect admixed individuals. |
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| format | Article |
| id | doaj.art-5fc846b7b25545c0960434269c5487c4 |
| institution | Directory Open Access Journal |
| issn | 2351-9894 |
| language | English |
| last_indexed | 2024-12-13T23:00:34Z |
| publishDate | 2021-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Global Ecology and Conservation |
| spelling | doaj.art-5fc846b7b25545c0960434269c5487c42022-12-21T23:28:25ZengElsevierGlobal Ecology and Conservation2351-98942021-08-0128e01681Genomic sequencing confirms absence of introgression despite past hybridisation between a critically endangered bird and its common congenerNatalie J. Forsdick0Denise Martini1Liz Brown2Hugh B. Cross3Richard F. Maloney4Tammy E. Steeves5Michael Knapp6Department of Anatomy, University of Otago, New Zealand; Correspondence to: Private Bag 92170, Auckland Mail Centre, Auckland 1142, New Zealand.Department of Anatomy, University of Otago, New ZealandNew Zealand Department of Conservation, Twizel, New ZealandDepartment of Anatomy, University of Otago, New ZealandNew Zealand Department of Conservation, Christchurch, New ZealandSchool of Biological Sciences, University of Canterbury, Christchurch, New ZealandDepartment of Anatomy, University of Otago, New ZealandGenetic swamping resulting from interspecific hybridisation can increase extinction risk for threatened species. The development of high-throughput and reduced-representation genomic sequencing and analyses to generate large numbers of high resolution genomic markers has the potential to reveal introgression previously undetected using small numbers of genetic markers. However, few studies to date have implemented genomic tools to assess the extent of interspecific hybridisation in threatened species. Here we investigate the utility of genome-wide single nucleotide polymorphisms (SNPs) to detect introgression resulting from past interspecific hybridisation in one of the world’s rarest birds. Anthropogenic impacts have resulted in hybridisation and subsequent backcrossing of the critically endangered Aotearoa New Zealand endemic kakī (black stilts; Himantopus novaezelandiae) with the non-threatened self-introduced congeneric poaka (Aotearoa New Zealand population of pied stilts, Himantopus himantopus leucocephalus), yet genetic analyses with a limited set of microsatellite markers revealed no evidence of introgression of poaka genetic material in kakī, excluding one individual. We use genomic data for ~63% of the wild adult kakī population to reassess the extent of introgression resulting from hybridisation between kakī and poaka. Consistent with previous genetic analyses, we detected no introgression from poaka into kakī. These collective results indicate that, for kakī, existing microsatellite markers provide a robust, cost-effective approach to detect cryptic hybrids. Further, for well-differentiated species, the use of genomic markers may not be required to detect admixed individuals.http://www.sciencedirect.com/science/article/pii/S2351989421002316Conservation genomicsInterspecific hybridisationConservation managementBlack Stilt |
| spellingShingle | Natalie J. Forsdick Denise Martini Liz Brown Hugh B. Cross Richard F. Maloney Tammy E. Steeves Michael Knapp Genomic sequencing confirms absence of introgression despite past hybridisation between a critically endangered bird and its common congener Global Ecology and Conservation Conservation genomics Interspecific hybridisation Conservation management Black Stilt |
| title | Genomic sequencing confirms absence of introgression despite past hybridisation between a critically endangered bird and its common congener |
| title_full | Genomic sequencing confirms absence of introgression despite past hybridisation between a critically endangered bird and its common congener |
| title_fullStr | Genomic sequencing confirms absence of introgression despite past hybridisation between a critically endangered bird and its common congener |
| title_full_unstemmed | Genomic sequencing confirms absence of introgression despite past hybridisation between a critically endangered bird and its common congener |
| title_short | Genomic sequencing confirms absence of introgression despite past hybridisation between a critically endangered bird and its common congener |
| title_sort | genomic sequencing confirms absence of introgression despite past hybridisation between a critically endangered bird and its common congener |
| topic | Conservation genomics Interspecific hybridisation Conservation management Black Stilt |
| url | http://www.sciencedirect.com/science/article/pii/S2351989421002316 |
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