Rapid adaptation of a complex trait during experimental evolution of Mycobacterium tuberculosis
Tuberculosis (TB), caused by Mycobacterium tuberculosis (M. tb), is a leading cause of death due to infectious disease. TB is not traditionally associated with biofilms, but M. tb biofilms are linked with drug and immune tolerance and there is increasing recognition of their contribution to the reca...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2022-06-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/78454 |
| _version_ | 1811252181514321920 |
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| author | Tracy M Smith Madison A Youngblom John F Kernien Mohamed A Mohamed Sydney S Fry Lindsey L Bohr Tatum D Mortimer Mary B O'Neill Caitlin S Pepperell |
| author_facet | Tracy M Smith Madison A Youngblom John F Kernien Mohamed A Mohamed Sydney S Fry Lindsey L Bohr Tatum D Mortimer Mary B O'Neill Caitlin S Pepperell |
| author_sort | Tracy M Smith |
| collection | DOAJ |
| description | Tuberculosis (TB), caused by Mycobacterium tuberculosis (M. tb), is a leading cause of death due to infectious disease. TB is not traditionally associated with biofilms, but M. tb biofilms are linked with drug and immune tolerance and there is increasing recognition of their contribution to the recalcitrance of TB infections. Here, we used M. tb experimental evolution to investigate this complex phenotype and identify candidate loci controlling biofilm formation. We identified novel candidate loci, adding to our understanding of the genetic architecture underlying M. tb biofilm development. Under selective pressure to grow as a biofilm, regulatory mutations rapidly swept to fixation and were associated with changes in multiple traits, including extracellular matrix production, cell size, and growth rate. Genetic and phenotypic paths to enhanced biofilm growth varied according to the genetic background of the parent strain, suggesting that epistatic interactions are important in M. tb adaptation to changing environments. |
| first_indexed | 2024-04-12T16:31:01Z |
| format | Article |
| id | doaj.art-5fc89de44b6d43769f6e64d4c6e6a35a |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:31:01Z |
| publishDate | 2022-06-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-5fc89de44b6d43769f6e64d4c6e6a35a2022-12-22T03:25:08ZengeLife Sciences Publications LtdeLife2050-084X2022-06-011110.7554/eLife.78454Rapid adaptation of a complex trait during experimental evolution of Mycobacterium tuberculosisTracy M Smith0Madison A Youngblom1https://orcid.org/0000-0003-4816-1113John F Kernien2Mohamed A Mohamed3https://orcid.org/0000-0003-4222-5112Sydney S Fry4Lindsey L Bohr5Tatum D Mortimer6https://orcid.org/0000-0001-6255-690XMary B O'Neill7Caitlin S Pepperell8https://orcid.org/0000-0002-6324-1333New York Genome Center, New York, United StatesDepartment of Medical Microbiology and Immunology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, United States; Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, United StatesDepartment of Medical Microbiology and Immunology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, United StatesDepartment of Medical Microbiology and Immunology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, United StatesDepartment of Medical Microbiology and Immunology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, United StatesDepartment of Medical Microbiology and Immunology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, United States; Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, United StatesDepartment of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, United StatesLaboratoire de Biochimie (LBC), Chimie Biologie et Innovation, ESPCI Paris, PSL Université, Paris, FranceDepartment of Medical Microbiology and Immunology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, United States; Department of Medicine (Infectious Diseases), School of Medicine and Public Health, University of Wisconsin-Madison, Madison, United StatesTuberculosis (TB), caused by Mycobacterium tuberculosis (M. tb), is a leading cause of death due to infectious disease. TB is not traditionally associated with biofilms, but M. tb biofilms are linked with drug and immune tolerance and there is increasing recognition of their contribution to the recalcitrance of TB infections. Here, we used M. tb experimental evolution to investigate this complex phenotype and identify candidate loci controlling biofilm formation. We identified novel candidate loci, adding to our understanding of the genetic architecture underlying M. tb biofilm development. Under selective pressure to grow as a biofilm, regulatory mutations rapidly swept to fixation and were associated with changes in multiple traits, including extracellular matrix production, cell size, and growth rate. Genetic and phenotypic paths to enhanced biofilm growth varied according to the genetic background of the parent strain, suggesting that epistatic interactions are important in M. tb adaptation to changing environments.https://elifesciences.org/articles/78454Mycobacterium tuberculosisbiofilmsexperimental evolutionpopulation genetics |
| spellingShingle | Tracy M Smith Madison A Youngblom John F Kernien Mohamed A Mohamed Sydney S Fry Lindsey L Bohr Tatum D Mortimer Mary B O'Neill Caitlin S Pepperell Rapid adaptation of a complex trait during experimental evolution of Mycobacterium tuberculosis eLife Mycobacterium tuberculosis biofilms experimental evolution population genetics |
| title | Rapid adaptation of a complex trait during experimental evolution of Mycobacterium tuberculosis |
| title_full | Rapid adaptation of a complex trait during experimental evolution of Mycobacterium tuberculosis |
| title_fullStr | Rapid adaptation of a complex trait during experimental evolution of Mycobacterium tuberculosis |
| title_full_unstemmed | Rapid adaptation of a complex trait during experimental evolution of Mycobacterium tuberculosis |
| title_short | Rapid adaptation of a complex trait during experimental evolution of Mycobacterium tuberculosis |
| title_sort | rapid adaptation of a complex trait during experimental evolution of mycobacterium tuberculosis |
| topic | Mycobacterium tuberculosis biofilms experimental evolution population genetics |
| url | https://elifesciences.org/articles/78454 |
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