Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K
A novel porous polyoxometalate (POM)-based composite (Co<sub>4</sub>PW–PDDVAC) was prepared via the solidification of water-soluble polytungstate (Co<sub>4</sub>PW) on the polymeric ionic liquid dimethyldodecyl-4-polyethylene benzyl ammonium chloride (PDDVAC) via a cation-exc...
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MDPI AG
2023-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/8/3307 |
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| author | Jiaxuan Yang Ning Chu Xuwei Chen |
| author_facet | Jiaxuan Yang Ning Chu Xuwei Chen |
| author_sort | Jiaxuan Yang |
| collection | DOAJ |
| description | A novel porous polyoxometalate (POM)-based composite (Co<sub>4</sub>PW–PDDVAC) was prepared via the solidification of water-soluble polytungstate (Co<sub>4</sub>PW) on the polymeric ionic liquid dimethyldodecyl-4-polyethylene benzyl ammonium chloride (PDDVAC) via a cation-exchange reaction. The solidification was confirmed by EDS, SEM, FT-IR, TGA, and so on. The strong covalent coordination and hydrogen-bonding interaction between the highly active Co<sup>2+</sup> of the Co<sub>4</sub>PW and the aspartic acid residues of proteinase K endowed the obtained Co<sub>4</sub>PW–PDDVAC composite with excellent proteinase K adsorption properties. Thermodynamic investigations indicate that the adsorption behavior of proteinase K was consistent with the linear Langmuir isothermal model, giving an adsorption capacity as high as 1428 mg g<sup>−1</sup>. The Co<sub>4</sub>PW–PDDVAC composite was applied in the selective isolation of highly active proteinase K from <i>Tritirachium album</i> Limber crude enzyme fluid. |
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| format | Article |
| id | doaj.art-5fcbc3a7c5464b4bb3964ce078654e3f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T04:42:31Z |
| publishDate | 2023-04-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-5fcbc3a7c5464b4bb3964ce078654e3f2023-11-17T20:37:06ZengMDPI AGMolecules1420-30492023-04-01288330710.3390/molecules28083307Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase KJiaxuan Yang0Ning Chu1Xuwei Chen2Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, ChinaBayuquan Customs District of the People’s Republic of China, Yingkou 115007, ChinaDepartment of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, ChinaA novel porous polyoxometalate (POM)-based composite (Co<sub>4</sub>PW–PDDVAC) was prepared via the solidification of water-soluble polytungstate (Co<sub>4</sub>PW) on the polymeric ionic liquid dimethyldodecyl-4-polyethylene benzyl ammonium chloride (PDDVAC) via a cation-exchange reaction. The solidification was confirmed by EDS, SEM, FT-IR, TGA, and so on. The strong covalent coordination and hydrogen-bonding interaction between the highly active Co<sup>2+</sup> of the Co<sub>4</sub>PW and the aspartic acid residues of proteinase K endowed the obtained Co<sub>4</sub>PW–PDDVAC composite with excellent proteinase K adsorption properties. Thermodynamic investigations indicate that the adsorption behavior of proteinase K was consistent with the linear Langmuir isothermal model, giving an adsorption capacity as high as 1428 mg g<sup>−1</sup>. The Co<sub>4</sub>PW–PDDVAC composite was applied in the selective isolation of highly active proteinase K from <i>Tritirachium album</i> Limber crude enzyme fluid.https://www.mdpi.com/1420-3049/28/8/3307polyoxometalatewater-soluble polytungstatepolymeric ionic liquidproteinase K (PrK)isolation |
| spellingShingle | Jiaxuan Yang Ning Chu Xuwei Chen Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K Molecules polyoxometalate water-soluble polytungstate polymeric ionic liquid proteinase K (PrK) isolation |
| title | Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K |
| title_full | Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K |
| title_fullStr | Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K |
| title_full_unstemmed | Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K |
| title_short | Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K |
| title_sort | preparation of polyoxometalate based composite by solidification of highly active cobalt containing polytungstate on polymeric ionic liquid for the efficient isolation of proteinase k |
| topic | polyoxometalate water-soluble polytungstate polymeric ionic liquid proteinase K (PrK) isolation |
| url | https://www.mdpi.com/1420-3049/28/8/3307 |
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