Identification of Renieramycin A as an Antileishmanial Substance in a Marine Sponge Neopetrosia sp.
Abstract: The newly developed assay system using recombinant Leishmania amazonensis expressing enhanced green fluorescent protein (La/egfp) has been applied to the screening of Japanese marine sponges for antileishmanial activity. Bioassay-guided fractionation of an active sponge Neopetrosia sp. aff...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2004-05-01
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| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1660-3397/2/2/55/ |
| _version_ | 1798039921505599488 |
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| author | Yoichi Nakao Takeru Shiroiwa Shigeki Matsunaga Shuhei Murayama Yasuyuki Goto Yoshitsugu Matsumoto Nobuhiro Fusetani |
| author_facet | Yoichi Nakao Takeru Shiroiwa Shigeki Matsunaga Shuhei Murayama Yasuyuki Goto Yoshitsugu Matsumoto Nobuhiro Fusetani |
| author_sort | Yoichi Nakao |
| collection | DOAJ |
| description | Abstract: The newly developed assay system using recombinant Leishmania amazonensis expressing enhanced green fluorescent protein (La/egfp) has been applied to the screening of Japanese marine sponges for antileishmanial activity. Bioassay-guided fractionation of an active sponge Neopetrosia sp. afforded an active compound which was identified as renieramycin A by spectroscopic analysis. It inhibited La/egfp with an IC50 value of 0.2 μg/mL. |
| first_indexed | 2024-04-11T22:00:22Z |
| format | Article |
| id | doaj.art-5fcc2f6b03e9441b83afb9d0e9e9e4c0 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T22:00:22Z |
| publishDate | 2004-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-5fcc2f6b03e9441b83afb9d0e9e9e4c02022-12-22T04:00:57ZengMDPI AGMarine Drugs1660-33972004-05-0122556210.3390/md202055Identification of Renieramycin A as an Antileishmanial Substance in a Marine Sponge Neopetrosia sp.Yoichi NakaoTakeru ShiroiwaShigeki MatsunagaShuhei MurayamaYasuyuki GotoYoshitsugu MatsumotoNobuhiro FusetaniAbstract: The newly developed assay system using recombinant Leishmania amazonensis expressing enhanced green fluorescent protein (La/egfp) has been applied to the screening of Japanese marine sponges for antileishmanial activity. Bioassay-guided fractionation of an active sponge Neopetrosia sp. afforded an active compound which was identified as renieramycin A by spectroscopic analysis. It inhibited La/egfp with an IC50 value of 0.2 μg/mL.http://www.mdpi.com/1660-3397/2/2/55/leishmaniasismarine spongerenieramycin A |
| spellingShingle | Yoichi Nakao Takeru Shiroiwa Shigeki Matsunaga Shuhei Murayama Yasuyuki Goto Yoshitsugu Matsumoto Nobuhiro Fusetani Identification of Renieramycin A as an Antileishmanial Substance in a Marine Sponge Neopetrosia sp. Marine Drugs leishmaniasis marine sponge renieramycin A |
| title | Identification of Renieramycin A as an Antileishmanial Substance in a Marine Sponge Neopetrosia sp. |
| title_full | Identification of Renieramycin A as an Antileishmanial Substance in a Marine Sponge Neopetrosia sp. |
| title_fullStr | Identification of Renieramycin A as an Antileishmanial Substance in a Marine Sponge Neopetrosia sp. |
| title_full_unstemmed | Identification of Renieramycin A as an Antileishmanial Substance in a Marine Sponge Neopetrosia sp. |
| title_short | Identification of Renieramycin A as an Antileishmanial Substance in a Marine Sponge Neopetrosia sp. |
| title_sort | identification of renieramycin a as an antileishmanial substance in a marine sponge neopetrosia sp |
| topic | leishmaniasis marine sponge renieramycin A |
| url | http://www.mdpi.com/1660-3397/2/2/55/ |
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