Genome-wide analysis of gene expression and protein secretion of Babesia canis during virulent infection identifies potential pathogenicity factors
Abstract Infections of dogs with virulent strains of Babesia canis are characterized by rapid onset and high mortality, comparable to complicated human malaria. As in other apicomplexan parasites, most Babesia virulence factors responsible for survival and pathogenicity are secreted to the host cell...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-03445-x |
| _version_ | 1818835179720933376 |
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| author | Ramon M. Eichenberger Chandra Ramakrishnan Giancarlo Russo Peter Deplazes Adrian B. Hehl |
| author_facet | Ramon M. Eichenberger Chandra Ramakrishnan Giancarlo Russo Peter Deplazes Adrian B. Hehl |
| author_sort | Ramon M. Eichenberger |
| collection | DOAJ |
| description | Abstract Infections of dogs with virulent strains of Babesia canis are characterized by rapid onset and high mortality, comparable to complicated human malaria. As in other apicomplexan parasites, most Babesia virulence factors responsible for survival and pathogenicity are secreted to the host cell surface and beyond where they remodel and biochemically modify the infected cell interacting with host proteins in a very specific manner. Here, we investigated factors secreted by B. canis during acute infections in dogs and report on in silico predictions and experimental analysis of the parasite’s exportome. As a backdrop, we generated a fully annotated B. canis genome sequence of a virulent Hungarian field isolate (strain BcH-CHIPZ) underpinned by extensive genome-wide RNA-seq analysis. We find evidence for conserved factors in apicomplexan hemoparasites involved in immune-evasion (e.g. VESA-protein family), proteins secreted across the iRBC membrane into the host bloodstream (e.g. SA- and Bc28 protein families), potential moonlighting proteins (e.g. profilin and histones), and uncharacterized antigens present during acute crisis in dogs. The combined data provides a first predicted and partially validated set of potential virulence factors exported during fatal infections, which can be exploited for urgently needed innovative intervention strategies aimed at facilitating diagnosis and management of canine babesiosis. |
| first_indexed | 2024-12-19T02:46:36Z |
| format | Article |
| id | doaj.art-5fcd6610a27542b29bb14c772125917a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T02:46:36Z |
| publishDate | 2017-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5fcd6610a27542b29bb14c772125917a2022-12-21T20:38:50ZengNature PortfolioScientific Reports2045-23222017-06-017111410.1038/s41598-017-03445-xGenome-wide analysis of gene expression and protein secretion of Babesia canis during virulent infection identifies potential pathogenicity factorsRamon M. Eichenberger0Chandra Ramakrishnan1Giancarlo Russo2Peter Deplazes3Adrian B. Hehl4Institute of Parasitology, University of ZurichInstitute of Parasitology, University of ZurichFunctional Genomics Center ZurichInstitute of Parasitology, University of ZurichInstitute of Parasitology, University of ZurichAbstract Infections of dogs with virulent strains of Babesia canis are characterized by rapid onset and high mortality, comparable to complicated human malaria. As in other apicomplexan parasites, most Babesia virulence factors responsible for survival and pathogenicity are secreted to the host cell surface and beyond where they remodel and biochemically modify the infected cell interacting with host proteins in a very specific manner. Here, we investigated factors secreted by B. canis during acute infections in dogs and report on in silico predictions and experimental analysis of the parasite’s exportome. As a backdrop, we generated a fully annotated B. canis genome sequence of a virulent Hungarian field isolate (strain BcH-CHIPZ) underpinned by extensive genome-wide RNA-seq analysis. We find evidence for conserved factors in apicomplexan hemoparasites involved in immune-evasion (e.g. VESA-protein family), proteins secreted across the iRBC membrane into the host bloodstream (e.g. SA- and Bc28 protein families), potential moonlighting proteins (e.g. profilin and histones), and uncharacterized antigens present during acute crisis in dogs. The combined data provides a first predicted and partially validated set of potential virulence factors exported during fatal infections, which can be exploited for urgently needed innovative intervention strategies aimed at facilitating diagnosis and management of canine babesiosis.https://doi.org/10.1038/s41598-017-03445-x |
| spellingShingle | Ramon M. Eichenberger Chandra Ramakrishnan Giancarlo Russo Peter Deplazes Adrian B. Hehl Genome-wide analysis of gene expression and protein secretion of Babesia canis during virulent infection identifies potential pathogenicity factors Scientific Reports |
| title | Genome-wide analysis of gene expression and protein secretion of Babesia canis during virulent infection identifies potential pathogenicity factors |
| title_full | Genome-wide analysis of gene expression and protein secretion of Babesia canis during virulent infection identifies potential pathogenicity factors |
| title_fullStr | Genome-wide analysis of gene expression and protein secretion of Babesia canis during virulent infection identifies potential pathogenicity factors |
| title_full_unstemmed | Genome-wide analysis of gene expression and protein secretion of Babesia canis during virulent infection identifies potential pathogenicity factors |
| title_short | Genome-wide analysis of gene expression and protein secretion of Babesia canis during virulent infection identifies potential pathogenicity factors |
| title_sort | genome wide analysis of gene expression and protein secretion of babesia canis during virulent infection identifies potential pathogenicity factors |
| url | https://doi.org/10.1038/s41598-017-03445-x |
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