Characterization of Extracellular Chitinase from Bacillus cereus SAHA 12.13 and Its Potency as a Biocontrol of Curvularia affinis
Bacillus cereus SAHA 12.13 can produce chitinase, an enzyme that digests chitin in the main compounds of cell walls, mycelia, and spores in pathogenic fungi that cause leaf spots on oil palm plants such as Curvularia affinis. This study aims to determine the properties of the chitinase enzyme B. cer...
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| Format: | Article |
| Language: | English |
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Pandawa Institute
2024-01-01
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| Series: | Journal of Multidisciplinary Applied Natural Science |
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| Online Access: | https://journal.pandawainstitute.com/index.php/jmans/article/view/203 |
| _version_ | 1797340286810062848 |
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| author | Muhammad Asril Didik Supriyadi |
| author_facet | Muhammad Asril Didik Supriyadi |
| author_sort | Muhammad Asril |
| collection | DOAJ |
| description | Bacillus cereus SAHA 12.13 can produce chitinase, an enzyme that digests chitin in the main compounds of cell walls, mycelia, and spores in pathogenic fungi that cause leaf spots on oil palm plants such as Curvularia affinis. This study aims to determine the properties of the chitinase enzyme B. cereus SAHA 12.13 that can inhibit the growth of C. affinis. Chitinase enzyme production and characterization were measured using the Spindler method. Antagonism test against pathogenic fungi using dual culture method by testing cell culture and enzyme crude extract. This result showed that the isolate produced a high level of specific chitinase activity at 37 °C for 45 h of incubation with 8.45 U mg-1 proteins with a growth rate (k) of 0.25 generation/h, and the generation time was 3.96 h/generation. The optimum chitinase activity was achieved at pH 7.0 and 45 °C and was stable for 3 h with a half-life (t1/2) of 770 min. The crude enzyme and cell culture of strain can inhibit the growth of C. affinis by 36.27±0.043% and 34.25±0.041%, respectively. These characteristics indicate that B. cereus strain SAHA12.13 can be used to inhibit C. affinis, which causes leaf blight of oil palm, under varying pH and temperature conditions. |
| first_indexed | 2024-03-08T10:00:47Z |
| format | Article |
| id | doaj.art-5fce146d9fb7448fb949a86dee9c2a0b |
| institution | Directory Open Access Journal |
| issn | 2774-3047 |
| language | English |
| last_indexed | 2024-03-08T10:00:47Z |
| publishDate | 2024-01-01 |
| publisher | Pandawa Institute |
| record_format | Article |
| series | Journal of Multidisciplinary Applied Natural Science |
| spelling | doaj.art-5fce146d9fb7448fb949a86dee9c2a0b2024-01-29T11:07:01ZengPandawa InstituteJournal of Multidisciplinary Applied Natural Science2774-30472024-01-014116517510.47352/jmans.2774-3047.203204Characterization of Extracellular Chitinase from Bacillus cereus SAHA 12.13 and Its Potency as a Biocontrol of Curvularia affinisMuhammad Asril0https://orcid.org/0000-0001-8637-4190Didik Supriyadi1https://orcid.org/0000-0002-5228-5197Department of Biology, Institut Teknologi Sumatera (ITERA), Lampung Selatan, Lampung-35365 (Indonesia); Microbiology Study Program, Department of Biology, IPB University, Bogor, West Java-16680 (Indonesia)Department of Chemical Engineering, Institut Teknologi Sumatera (ITERA), Lampung Selatan, Lampung-35365 (Indonesia)Bacillus cereus SAHA 12.13 can produce chitinase, an enzyme that digests chitin in the main compounds of cell walls, mycelia, and spores in pathogenic fungi that cause leaf spots on oil palm plants such as Curvularia affinis. This study aims to determine the properties of the chitinase enzyme B. cereus SAHA 12.13 that can inhibit the growth of C. affinis. Chitinase enzyme production and characterization were measured using the Spindler method. Antagonism test against pathogenic fungi using dual culture method by testing cell culture and enzyme crude extract. This result showed that the isolate produced a high level of specific chitinase activity at 37 °C for 45 h of incubation with 8.45 U mg-1 proteins with a growth rate (k) of 0.25 generation/h, and the generation time was 3.96 h/generation. The optimum chitinase activity was achieved at pH 7.0 and 45 °C and was stable for 3 h with a half-life (t1/2) of 770 min. The crude enzyme and cell culture of strain can inhibit the growth of C. affinis by 36.27±0.043% and 34.25±0.041%, respectively. These characteristics indicate that B. cereus strain SAHA12.13 can be used to inhibit C. affinis, which causes leaf blight of oil palm, under varying pH and temperature conditions.https://journal.pandawainstitute.com/index.php/jmans/article/view/203bacillus cereuscharacterizationchitinasecurvularia affinisenvironmental factorsnatural resource |
| spellingShingle | Muhammad Asril Didik Supriyadi Characterization of Extracellular Chitinase from Bacillus cereus SAHA 12.13 and Its Potency as a Biocontrol of Curvularia affinis Journal of Multidisciplinary Applied Natural Science bacillus cereus characterization chitinase curvularia affinis environmental factors natural resource |
| title | Characterization of Extracellular Chitinase from Bacillus cereus SAHA 12.13 and Its Potency as a Biocontrol of Curvularia affinis |
| title_full | Characterization of Extracellular Chitinase from Bacillus cereus SAHA 12.13 and Its Potency as a Biocontrol of Curvularia affinis |
| title_fullStr | Characterization of Extracellular Chitinase from Bacillus cereus SAHA 12.13 and Its Potency as a Biocontrol of Curvularia affinis |
| title_full_unstemmed | Characterization of Extracellular Chitinase from Bacillus cereus SAHA 12.13 and Its Potency as a Biocontrol of Curvularia affinis |
| title_short | Characterization of Extracellular Chitinase from Bacillus cereus SAHA 12.13 and Its Potency as a Biocontrol of Curvularia affinis |
| title_sort | characterization of extracellular chitinase from bacillus cereus saha 12 13 and its potency as a biocontrol of curvularia affinis |
| topic | bacillus cereus characterization chitinase curvularia affinis environmental factors natural resource |
| url | https://journal.pandawainstitute.com/index.php/jmans/article/view/203 |
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