Can antimicrobial blue light contribute to resistance development? Genome-wide analysis revealed aBL-protective genes in Escherichia coli
ABSTRACT Antimicrobial blue light (aBL) is a promising non-antibiotic approach to fighting multidrug-resistant bacteria. However, the complete mechanism of aBL action is not fully understood yet. This study contributes to a better understanding that the response to aBL depends on many factors and th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2024-01-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.02490-23 |
| _version_ | 1797357430622912512 |
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| author | Beata Kruszewska-Naczk Mariusz Grinholc Krzysztof Waleron Julia Elisabeth Bandow Aleksandra Rapacka-Zdończyk |
| author_facet | Beata Kruszewska-Naczk Mariusz Grinholc Krzysztof Waleron Julia Elisabeth Bandow Aleksandra Rapacka-Zdończyk |
| author_sort | Beata Kruszewska-Naczk |
| collection | DOAJ |
| description | ABSTRACT Antimicrobial blue light (aBL) is a promising non-antibiotic approach to fighting multidrug-resistant bacteria. However, the complete mechanism of aBL action is not fully understood yet. This study contributes to a better understanding that the response to aBL depends on many factors and that it is hardly possible to identify a predominant mechanism underlying microbial sensitivity to photoinactivation. The results of this study provide insights into genetic changes that may lead to bacterial survival at higher aBL doses, giving rise to aBL-resistant strains. To our best knowledge, this is the first study concerning genome-wide mutant testing of aBL. We managed to identify 64 single-gene mutants that lacked certain protective genes expressing aBL-increased sensitivity. IMPORTANCE Increasing antibiotic resistance and the lack of new antibiotic-like compounds to combat bacterial resistance are significant problems of modern medicine. The development of new alternative therapeutic strategies is extremely important. Antimicrobial blue light (aBL) is an innovative approach to combat multidrug-resistant microorganisms. aBL has a multitarget mode of action; however, the full mechanism of aBL antibacterial action requires further investigation. In addition, the potential risk of resistance development to this treatment should be considered. |
| first_indexed | 2024-03-08T14:43:55Z |
| format | Article |
| id | doaj.art-5fce25dbb36b46a49654b2919c8d3d90 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-08T14:43:55Z |
| publishDate | 2024-01-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-5fce25dbb36b46a49654b2919c8d3d902024-01-11T14:04:37ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972024-01-0112110.1128/spectrum.02490-23Can antimicrobial blue light contribute to resistance development? Genome-wide analysis revealed aBL-protective genes in Escherichia coliBeata Kruszewska-Naczk0Mariusz Grinholc1Krzysztof Waleron2Julia Elisabeth Bandow3Aleksandra Rapacka-Zdończyk4Laboratory of Photobiology and Molecular Diagnostics, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk , Gdansk, PolandLaboratory of Photobiology and Molecular Diagnostics, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk , Gdansk, PolandDepartment of Pharmaceutical Microbiology, Faculty of Pharmacy, Medical University of Gdansk , Gdansk, PolandApplied Microbiology, Faculty of Biology and Biotechnology, Ruhr University Bochum, Universitätsstraße , Bochum, GermanyDepartment of Pharmaceutical Microbiology, Faculty of Pharmacy, Medical University of Gdansk , Gdansk, PolandABSTRACT Antimicrobial blue light (aBL) is a promising non-antibiotic approach to fighting multidrug-resistant bacteria. However, the complete mechanism of aBL action is not fully understood yet. This study contributes to a better understanding that the response to aBL depends on many factors and that it is hardly possible to identify a predominant mechanism underlying microbial sensitivity to photoinactivation. The results of this study provide insights into genetic changes that may lead to bacterial survival at higher aBL doses, giving rise to aBL-resistant strains. To our best knowledge, this is the first study concerning genome-wide mutant testing of aBL. We managed to identify 64 single-gene mutants that lacked certain protective genes expressing aBL-increased sensitivity. IMPORTANCE Increasing antibiotic resistance and the lack of new antibiotic-like compounds to combat bacterial resistance are significant problems of modern medicine. The development of new alternative therapeutic strategies is extremely important. Antimicrobial blue light (aBL) is an innovative approach to combat multidrug-resistant microorganisms. aBL has a multitarget mode of action; however, the full mechanism of aBL antibacterial action requires further investigation. In addition, the potential risk of resistance development to this treatment should be considered.https://journals.asm.org/doi/10.1128/spectrum.02490-23antimicrobial blue lightEscherichia coligenome-wide analysishypersensitive mutantsresistance |
| spellingShingle | Beata Kruszewska-Naczk Mariusz Grinholc Krzysztof Waleron Julia Elisabeth Bandow Aleksandra Rapacka-Zdończyk Can antimicrobial blue light contribute to resistance development? Genome-wide analysis revealed aBL-protective genes in Escherichia coli Microbiology Spectrum antimicrobial blue light Escherichia coli genome-wide analysis hypersensitive mutants resistance |
| title | Can antimicrobial blue light contribute to resistance development? Genome-wide analysis revealed aBL-protective genes in Escherichia coli |
| title_full | Can antimicrobial blue light contribute to resistance development? Genome-wide analysis revealed aBL-protective genes in Escherichia coli |
| title_fullStr | Can antimicrobial blue light contribute to resistance development? Genome-wide analysis revealed aBL-protective genes in Escherichia coli |
| title_full_unstemmed | Can antimicrobial blue light contribute to resistance development? Genome-wide analysis revealed aBL-protective genes in Escherichia coli |
| title_short | Can antimicrobial blue light contribute to resistance development? Genome-wide analysis revealed aBL-protective genes in Escherichia coli |
| title_sort | can antimicrobial blue light contribute to resistance development genome wide analysis revealed abl protective genes in escherichia coli |
| topic | antimicrobial blue light Escherichia coli genome-wide analysis hypersensitive mutants resistance |
| url | https://journals.asm.org/doi/10.1128/spectrum.02490-23 |
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